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VP Test: A Microbiology Technique for Detecting Acetoin Production in Bacteria, Slides of Microbiology

The VP test is a microbiology technique used to detect the production of acetoin from glucose fermentation by certain bacteria. the procedure for performing the test, including the required apparatus, ingredients, and expected results. It also provides examples of bacteria that give positive and negative results in the VP test.

What you will learn

  • What is the purpose of the VP test in microbiology?
  • Which bacteria produce acetoin during glucose fermentation?
  • What are the expected results of a VP test?

Typology: Slides

2021/2022

Uploaded on 09/27/2022

sergeybrin
sergeybrin 🇺🇸

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Name of the Student: SMITA D.
Class- TY MICROBIOLOGY
Under The Guidance of Chitra
Nair
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Name of the Student: SMITA D. Class- TY MICROBIOLOGY Under The Guidance of Chitra Nair

Sr

no

Name of apparatus volume Quantity

Media & Reagents

Sterile Glucose Phosphate Broth

5ml _

2 40 % Koh 2ml

_

3 Alpha Naphthol 2ml

_

4 Provided Sample 1ml

_

Miscellaneous

Nichrome Wire Loop

_ _

6 Incubator at 37 ◦C for 24 hrs

_ _

Protocol :

Inoculate loopful sample in sterile glucose phosphate broth.

Incubate it at 37 ◦c for 24 hrs.

Add 0.5 ml of 5% alpha naphthol ,0.2 ml 40% KOH Shake gently.

Allow to remain undisturbed

  • Red color within 15

mins indicates

positive result

  • Yellow color indicates

negative result

 Results of VP tests need to be used in conjunction with other

biochemical tests to differentiate genus and species within the Enterobacteriaceae.

 VP reagents must be added in the order.

 A weak-positive reaction may be masked by a copper-like color

which may form due to the reaction of KOH and a-naphthol.

 Read the VP test within 1 hour of adding the reagents. The KOH

and a-naphthol may react to form a copper-like color.

 Some organisms destroy acetoin, thereby rendering the VP tests

invalid.

 Shaking the tubes enhances the VP reaction.