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The VP test is a microbiology technique used to detect the production of acetoin from glucose fermentation by certain bacteria. the procedure for performing the test, including the required apparatus, ingredients, and expected results. It also provides examples of bacteria that give positive and negative results in the VP test.
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Name of the Student: SMITA D. Class- TY MICROBIOLOGY Under The Guidance of Chitra Nair
Sterile Glucose Phosphate Broth
5ml _
2 40 % Koh 2ml
3 Alpha Naphthol 2ml
4 Provided Sample 1ml
Nichrome Wire Loop
6 Incubator at 37 ◦C for 24 hrs
mins indicates
positive result
negative result
Results of VP tests need to be used in conjunction with other
biochemical tests to differentiate genus and species within the Enterobacteriaceae.
VP reagents must be added in the order.
A weak-positive reaction may be masked by a copper-like color
which may form due to the reaction of KOH and a-naphthol.
Read the VP test within 1 hour of adding the reagents. The KOH
and a-naphthol may react to form a copper-like color.
Some organisms destroy acetoin, thereby rendering the VP tests
invalid.
Shaking the tubes enhances the VP reaction.