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Unit 7 Lab 2: Cheek Cells, Study Guides, Projects, Research of Pharmacy

University of the District of Columbia (UDC)Pharmacy

Objectives: •. Demonstrate the proper procedures used in correctly using the compound light microscope. •. Prepare and use a wet mount of living cells. •. Stain ...

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2021/2022

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Unit 7 Lab 2: Cheek Cells
Name___________________________Date_________________Period____________
Introduction:
Many things that are viewed using a microscope, particularly cells, can appear quite
transparent under the microscope. The internal parts of the cells, the organelles, are so
transparent that they are often difficult to see. Biologists have developed a number of stains that
help them see the cells and their organelles by adding color to their transparent parts.
While many biological stains are for advanced study only, there are some that are easy to
obtain and use. Some readily available stains are: food coloring, iodine, malachite green (Ick fish
cure), and methylene blue. Food coloring can be found at a grocery store, and iodine can be
found at a pharmacy. The last two stains, malachite green and methylene blue can be purchased
at aquarium shops.
Interestingly, certain stains color certain parts of a cell. Scientists choose specific stains
when they want to look at a particular part of a cell. You will be experimenting with the two of the
stains listed above to see which parts of your cheek cells each one colors.
Objectives:
• Demonstrate the proper procedures used in correctly using the compound light
microscope.
• Prepare and use a wet mount of living cells.
• Stain a wet mounted sample
• Identify cellular organelles in a stained tissue sample
Materials:
• Microscope
• Eyedropper
• 2 flat slides
• 2 cover slips
• Toothpick
• Cheek cells
• Stain (Lugol’s solution or methylene blue)
• Paper towel
• Water
• Pencil
• Paper
• Eraser
• Dilute bleach water in a beaker
Procedures:
1. Before you begin, decide which stain you will use for the experiment (Lugol’s solution or
methylene blue). After you have completed your work discard the cover slip and soak the
slide in the bleach solution for 5 minutes. RINSE & DRY YOUR OWN SLIDE BEFORE
YOU LEAVE!
2. Cells from the inside lining of your cheek are good for learning how to stain. Gently scrape
the inside of your mouth with the flat side of a toothpick. This scraping will collect some of
your cheek cells. (Don't worry; these cells are constantly being shed from your mouth so
they will not be missed!)
pf3

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Download Unit 7 Lab 2: Cheek Cells and more Study Guides, Projects, Research Pharmacy in PDF only on Docsity!

Unit 7 Lab 2: Cheek Cells

Name___________________________Date_________________Period____________

Introduction : Many things that are viewed using a microscope, particularly cells, can appear quite transparent under the microscope. The internal parts of the cells, the organelles, are so transparent that they are often difficult to see. Biologists have developed a number of stains that help them see the cells and their organelles by adding color to their transparent parts. While many biological stains are for advanced study only, there are some that are easy to obtain and use. Some readily available stains are: food coloring, iodine, malachite green (Ick fish cure), and methylene blue. Food coloring can be found at a grocery store, and iodine can be found at a pharmacy. The last two stains, malachite green and methylene blue can be purchased at aquarium shops. Interestingly, certain stains color certain parts of a cell. Scientists choose specific stains when they want to look at a particular part of a cell. You will be experimenting with the two of the stains listed above to see which parts of your cheek cells each one colors.

Objectives :

  • Demonstrate the proper procedures used in correctly using the compound light microscope.
  • Prepare and use a wet mount of living cells.
  • Stain a wet mounted sample
  • Identify cellular organelles in a stained tissue sample

Materials :

  • Microscope
  • Eyedropper
  • 2 flat slides
  • 2 cover slips
  • Toothpick
  • Cheek cells
    • Stain (Lugol’s solution or methylene blue)
    • Paper towel
    • Water
    • Pencil
    • Paper
    • Eraser
    • Dilute bleach water in a beaker

Procedures :

1. Before you begin, decide which stain you will use for the experiment (Lugol’s solution or methylene blue). After you have completed your work discard the cover slip and soak the slide in the bleach solution for 5 minutes. RINSE & DRY YOUR OWN SLIDE BEFORE YOU LEAVE!

  1. Cells from the inside lining of your cheek are good for learning how to stain. Gently scrape the inside of your mouth with the flat side of a toothpick. This scraping will collect some of your cheek cells. (Don't worry; these cells are constantly being shed from your mouth so they will not be missed!)
  1. Place the cells on a flat slide and make a wet mount using water and a cover slip as you did in the Lab 1 when you made a wet mount of the lower case “e”.
  2. Now repeat this procedure so that you have two wet mounts of cheek cells (one for each partner in you group).
  3. Now you need to add stain to one slide. To add stain, put a drop of the stain next to the cover slip on the slide and then draw it under the cover slip by placing a piece of paper towel against the other side of the cover slip. The paper towel will soak up the water, drawing the stain under the cover slip around the cell. Drawing the stain under the cell is called "wicking."
  4. Use the Scanning/Low power/ 4x objective to focus, look at the stained slide under the microscope. You probably will not see the cells at this power. Sketch what you see below and place the magnification next to the drawing.
  5. Switch to low power. Cells should be visible, but they will be small and look like nearly clear blobs (stained cells will be easier to see). Solid dark objects are probably not cellular. Sketch what you see below and place the magnification next to the drawing.
  6. Once you think you have located a cell, switch to a high power and refocus. (Remember; do NOT use the coarse adjustment knob at this point). Sketch what you see below and place the magnification next to the drawing.

Sketch the stained cells at scanning, low and high power. Label the nucleus, cytoplasm, and cell membrane. Draw your cells to scale and include the magnification.

Scanning (______x) Low (______x) High (______x)