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Chapter 21 Answers and Solutions
Answers and Solutions to Text Problems
21.1 The chemical reactions can occur without enzymes, but the rates are too slow. Catalyzed reactions, which are many times faster, provide the amounts of products needed by the cell at a particular time.
21.2 Enzymes lower the energy of activation for a reaction, which allows more reacting molecules to form product.
21.3 a. Oxidoreductases catalyze oxidation and reduction. b. Transferases move groups such as amino or phosphate groups from one substance to another. c. Hydrolases use water to split bonds in molecules such as carbohydrates, peptides, and lipids.
21.4 a. addition or removal of a group to or from a double bond without hydrolysis or oxidation b. rearrangement of atoms to form isomers c. bonding of molecules using ATP energy.
21.5 a. A hydrolase enzyme would catalyze the hydrolysis of sucrose. b. An oxidoreductase enzyme would catalyze the addition of oxygen (oxidation). c. An isomerase enzyme would catalyze converting glucose to fructose. d. A transferase enzyme would catalyze moving an amino group. 21.6 a. lyase b. oxidoreductase c. hydrolase d. lyase
21.7 a. A lyase such as a decarboxylase removes CO 2 from a molecule. b. The transfer of an amino group to another molecule would be catalyzed by a transferase.
21.8 a. ligase b. oxidoreductase
21.9 a. Succinate oxidase catalyzes the oxidation of succinate. b. Fumarate hydrase catalyzes the addition of water to fumarate. c. Alcohol dehydrogenase removes 2H from an alcohol.
21.10 a. sucrase b. aspartate transferase (transaminase) c. pyruvate decarboxylase
21.11 a. An enzyme has a tertiary structure that recognized the substrate. b. The combination of the enzyme and substrate is the enzyme-substrate complex. c. The substrate has a structure that complements the structure of the enzyme.
21.12 a. The active site (1) on an enzyme is where catalytic activity occurs. b. In the induced-fit model (3), the active site adjusts to the substrate shape. c. In the lock-and-key model (2), the active site is considered to have a rigid shape that only fits a substrate with that geometry.
21.13 a. The equation for an enzyme-catalyzed reaction is: E + S ES E + P E = enzyme, S = substrate, ES = enzyme-substrate complex, P = products b. The active site is a region or pocket within the tertiary structure of an enzyme that accepts the substrate, aligns the substrate for reaction, and catalyzes the reaction.
21.14 a. An enzyme speeds up the reaction of substrate because an enzyme lowers the activation energy for the reaction of that substrate.
Enzymes
b. As soon as the enzyme releases product, the enzyme is available to catalyze the reaction of more substrate
21.15 Isoenzymes are slightly different forms of an enzyme that catalyze the same reaction in different organs and tissues of the body.
21.16 The LDH isoenzyme in the heart consists of four H polypeptide subunits, whereas the LDH isoenzyme in the liver consists of four M polypeptide subunits.
21.17 A doctor might run tests for the enzymes CK, LDH, and AST to determine if the patient had a heart attack.
21.18 LDH could indicate liver damage, and AST could indicate cirrhosis or hepatitis.
21.19 a. Decreasing the substrate concentration decreases the rate of reaction. b. Running the reaction at a pH below optimum pH will decrease the rate of reaction. c. Temperature above 37°C (optimum pH) will denature the enzymes and decrease the rate of reaction. d. Increasing the enzyme concentration would increase the rate of reaction.
21.20 a. increases rate b. decreases rate c. decreases rate d. decreases rate
21.21 pepsin, pH 2; urease, pH 5; trypsin; pH 8
21.22 a. Trypsin has an optimum pH at about pH 9; pH 5 is not the optimum pH. b. Yes, urease is at its optimum pH of 5 c. Pepsin has an optimum pH at about pH 1.5; pH 4 is not the optimum pH. d. Trypsin has an optimum pH at about pH 8; pH 8 is the optimum pH. e. Pepsin has an optimum pH of about pH 2; pH 2 is optimum pH
21.23 a. If the inhibitor has a structure similar to the structure of the substrate, the inhibitor is competitive b. If adding more substrate cannot reverse the effect of the inhibitor, the inhibitor is noncompetitive. c. If the inhibitor competes with the substrate for the active site, it is a competitive inhibitor. d. If the structure of the inhibitor is not similar to the structure of the substrate, the inhibitor is noncompetitive. e. If adding more substrate reverses inhibition, the inhibitor is competitive.
21.24 a. It would be a reversible competitive inhibitor because the structure of oxaloacetate is similar to the structure of succinate, the substrate. b. As a competitive inhibitor, oxaloacetate would fit into the active site. c. Increasing the concentration can reverse the effect of the competitive inhibitor succinate, the substrate for the reaction.
21.25 a. Methanol has the structural formula CH 3 OH whereas ethanol is CH 3 CH 2 OH. b. Ethanol has a structure similar to methanol and could compete for the active site. c. Ethanol is a competitive inhibitor of methanol oxidation.
21.26 a. No; the antibiotic only inhibits enzymes in bacteria. b. It inhibits an enzyme required to build cell walls in bacteria, but not in human cells. c. Antibiotics are irreversible inhibitors.
21.27 Digestive enzymes are proteases and would digest the proteins of the organ where they are produced if they were active immediately upon synthesis.
Enzymes
21.47 The many different reactions that take place in cells require different enzymes because enzymes react with only a certain type of substrate.
21.48 No. Enzymes are synthesized when needed in the cell and broken down when not needed. It would be inefficient for a cell to tie up atoms in enzymes that are not needed for catalysis.
21.49 When exposed to conditions of strong acids or bases, or high temperatures, enzymatic proteins are denatured rapidly causing a loss of tertiary structure and catalytic activity.
21.50 Enzymes are proteins, which are denatured in acidic or basic conditions and by high temperatures.
21.51 a. The reactant is lactose and the products are glucose and galactose.
Energy of activation without enzyme Energy of activation with enzyme b. Lactose
Products: glucose and galactose Reaction progress →
c. By lowering the energy of activation, the enzyme furnishes a lower energy pathway by which the reaction can take place.
21.52 a. maltose → 2 glucose
b. Energy of activation without maltase
Energy of activation with maltase Maltose Products: two glucose
Reaction progress →
c. Maltase lowers the activation energy for the hydrolysis of maltose.
21.53 a. The disaccharide lactose is a substrate. b. The – ase in lactase indicates that it is an enzyme. c. The – ase in urease indicates that it is an enzyme. d. Trypsin is an enzyme, which hydrolyzes polypeptides. e. Pyruvate is a substrate. f. The – ase in transaminase indicates that it is an enzyme.
21.54 a. substrate (S) b. enzyme (E) c. enzyme (E) d. substrate (S) e. substrate (S) f. enzyme (E)
21.55 a. Urea is the substrate of urease. b. Lactose is the substrate of lactase. c. Aspartate is the substrate of aspartate transaminase. d. Tyrosine is the substrate of tyrosine synthetase.
Chapter 21 Answers and Solutions
21.56 a. maltose b. fructose c. phenol d. sucrose
21.57 a. The transfer of an acyl group is catalyzed by a transferase. b. Oxidases are classified as oxidoreductases. c. A lipase, which splits esters bonds in lipids with water, is a hydrolase. d. A decarboxylase is classified as a lyase.
21.58 a. isomerase b. oxidoreductase c. ligase d. hydrolase
21.59 a. In this reaction, oxygen is added to an aldehyde. The enzyme that catalyzes this reaction would be an oxidoreductase. b. In this reaction, a dipeptide is hydrolyzed. The enzyme that catalyzes this reaction would be a hydrolase. c. In this reaction, water is added to a double bond. The enzyme that catalyzes this reaction would be a lyase.
21.60 a An enzyme that catalyzes removal of a group and involves a double bond is a lyase. b In this reaction, carbon dioxide is added to a ketoacid using ATP. The enzyme that catalyzes this reaction would be a ligase. c The conversion of glucose-6-phosphate to fructose-6-phosphate is an isomerization and an isomerase would be the enzyme that catalyzes the reaction.
21.61 Sucrose fits the shape of the active site in sucrase, but lactose does not.
21.62 In the induced-fit model, the active site adjusts to fit the shape of the substrate; the substrate adjusts it shape to better fit the active site. Thus, one enzyme can catalyze a group of substrates.
21.63 A heart attack may be the cause. Normally the enzymes LDH and CK are present only in low levels in the blood.
21.64 An elevated ALT (alanine transaminase) level could indicate hepatitis.
21.65 a. An enzyme is saturated if adding more substrate does not increase the rate. b. An enzyme is unsaturated when increasing the substrate increases the rate.
21.66 a. Pepsin is functional at pH 2, which is its optimal pH. b. This enzyme is not functional at 37°C because it has an optimum temperature of 100°C.
21.67 In a reversible inhibition, the inhibitor can dissociate from the enzyme, whereas in irreversible inhibition, the inhibitor forms a strong covalent bond with the enzyme and does not dissociate. Irreversible inhibitors act as poisons to enzymes.
21.68 The inhibitor in competitive reversible inhibition competes for the active site because it has a structure similar to the substrate. Increasing the substrate concentration reverses the inhibition. The inhibitor in noncompetitive inhibition is not similar to the substrate and does not compete for the active site. Increasing the substrate concentration does not reverse the inhibition. To re- establish enzyme activity, the concentration of the inhibitor must decrease.
21.69 a. The oxidation of glycol to an aldehyde and carboxylic acid is catalyzed by an oxidoreductase. b. At high concentration, ethanol, which acts as a competitive inhibitor of ethylene glycol, would saturate the enzyme to allow ethylene glycol to be removed from the body without producing oxalic acid.
21.70 a. Lactase is the enzyme that hydrolyzes milk; it is a hydrolase. b. Enzymes such as lactase are denatured at high temperatures.
21.71 a. Antibiotics such as amoxicillin are irreversible inhibitors.
Chapter 21 Answers and Solutions
21.84 There is a daily requirement for water-soluble vitamins because many are used by enzymes as cofactors to carry out enzyme-catalyzed reactions. Because the water-soluble vitamins are not stored in the body, they must be replenished each day.
21.85 a. A deficiency of niacin can lead to pellagra. b. A deficiency of vitamin A can lead to night blindness. c. A deficiency of vitamin D can weaken bone structure.
21.86 a. A deficiency of cobalamin is associated with anemia. b. A deficiency of vitamin C can lead to scurvy. c. A deficiency of vitamin K can lead to increased bleeding.
