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Recombinant DNA Technology and Gene Cloning - Notes | CHEM 2011, Study notes of Organic Chemistry

East Tennessee State University (ETSU)Organic Chemistry

Notes Material Type: Notes; Class: Organic Chemistry Lab I; Subject: Chemistry (CHEM); University: East Tennessee State University; Term: Fall 2009;

Typology: Study notes

2009/2010

Uploaded on 12/09/2010

jcodyhall
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Copyright © 2009 Pearson Education, Inc.
PowerPoint® Lecture Presentation for
Concepts of Genetics
Ninth Edition
Klug, Cummings, Spencer, Palladino
Chapter 13
Recombinant DNA Technology
and Gene Cloning
Lectures by David Kass with contributions from
John C. Osterman.
Copyright © 2009 Pearson Education, Inc.
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Download Recombinant DNA Technology and Gene Cloning - Notes | CHEM 2011 and more Study notes Organic Chemistry in PDF only on Docsity!

Copyright © 2009 Pearson Education, Inc. PowerPoint ® Lecture Presentation for

Concepts of Genetics

Ninth Edition Klug, Cummings, Spencer, Palladino Chapter 13 Recombinant DNA Technology and Gene Cloning Lectures by David Kass with contributions from John C. Osterman.

Chapter Contents

13.1 Recombinant DNA Technology Combines Several Laboratory Techniques 13.2 Restriction Enzymes Cut DNA at Specific Recognition Sequences 13.3 Vectors Carry DNA Molecules to Be Cloned 13.4 DNA Was First Cloned in Prokaryotic Cells 13.5 Yeast Cells Are Used as Eukaryotic Hosts for Cloning Continued

13.1 Recombinant DNA Technology Combines Several Laboratory Techniques

  • (^) Recombinant DNA refers to the joining of DNA molecules, usually from different biological sources, that are not found together in nature.

Section 13.

  • (^) The recovered copies of a recombinant DNA molecule are referred to as clones , and can be used to study the structure and orientation of the DNA.
  • (^) Recombinant DNA technology is used to isolate, replicate, and analyze genes.

Section 13.

13.2 Restriction Enzymes Cut DNA at Specific Recognition Sequences

  • (^) DNA ligase joins restriction fragments covalently to produce intact DNA molecules ( Figure 13.3 ).

Section 13.

13.3 Vectors Carry DNA Molecules to Be Cloned

  • (^) Vectors are carrier DNA molecules that can replicate cloned DNA fragments in a host cell.
  • (^) Vectors must be able to replicate independently and should have several restriction enzyme sites to allow insertion of a DNA fragment.

Section 13.

  • Copyright © 2009 Pearson Education, Inc. Figure 13.
  • Copyright © 2009 Pearson Education, Inc. Figure 13.
  • Copyright © 2009 Pearson Education, Inc. Figure 13.
  • Copyright © 2009 Pearson Education, Inc. Figure 13.
  • Copyright © 2009 Pearson Education, Inc. Figure 13.
  • (^) Plasmids used for DNA cloning usually have been engineered to contain: - (^) a number of convenient restriction sites - (^) a marker gene to select for its presence in the host cell ( Figure 13.5 , Figure 13.6 , and Figure 13.7 )

Section 13.