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Spring 2011 Bio 1100 Quiz 5: Understanding Cellular Processes - Prof. Jason M. Gee, Quizzes of Biology

A quiz for the bio 1100 class, focusing on various cellular processes such as flagellum movement, protein transport, and cytoskeleton dynamics. Students are asked to identify the expected observation when the plasma membrane around a flagellum is opened and dynein sidearms are removed, infer the fate of high-molecular-weight proteins that cannot enter the nucleus, and explain the function of the nuclear pore complex, among other questions.

Typology: Quizzes

2010/2011

Uploaded on 04/25/2011

bmmcgirt
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Spring 2011 Bio 1100 Sec03 Quiz 5 Key (blackboard quiz 3)
1. Suppose that the plasma membrane around a flagellum is opened to reveal the axoneme inside. The dynein
sidearms between the peripheral microtubule doublets are then removed by chemical treatment. ATP is then
added. What is the expected observation?
A. The axoneme will elongate or grow in size.
B. There will be normal bending of the flagellum.
C. There will be no movement, since the plasma membrane is not present.
D. There will be no movement, because the ability to use ATP is lost.
2. What can you infer about high-molecular-weight proteins that cannot be transported into the nucleus?
A. They are foreign proteins. B. They lack nuclear localization signals (NLS).
C. They are defective proteins. D. They have been tagged for destruction.
3. What is the function of the nuclear pore complex?
A. It regulates movement of materials across the nuclear envelope.
B. It assembles ribosomes from raw materials that are synthesized in the nucleus.
C. It synthesizes components of the endomembrane system.
D. It synthesizes and repairs DNA.
4. Scientists found that polypeptides that are normally synthesized in the endoplasmic reticulum are about 20
amino acids longer when they are synthesized by ribosomes not attached to the endoplasmic reticulum. What
is/are possible explanations for the greater length of these polypeptides?
A. The ribosomes that function as free ribosomes function differently than the ribosomes that are
attached to the endoplasmic reticulum.
B. The 20 amino acids serve as a signal sequence that directs the forming polypeptide to the
endoplasmic reticulum, where they are cleaved off during processing.
C. The 20-amino-acid sequence helps the endoplasmic reticulum package these proteins for shipping to
the Golgi.
D. The protein has a different function in the cytosol than in the endoplasmic reticulum.
5. Researchers tried to explain how vesicular transport occurs in cells by attempting to assemble the transport
components. They set up microtubular tracks along which vesicles could be transported, and they added vesicles
and ATP (because they knew the transport process requires energy). Yet, when they put everything together,
there was no movement or transport of vesicles. What were they missing?
A. an axon B. contractile filaments C. motor proteins D. rough endoplasmic reticulum
6. Amoeba move by crawling over a surface (amoeboid movement). Which one of the following processes is part
of amoeboid movement?
A. polymerization or extension of actin filaments to form pseudopodia or “false feet” in the plasma
membrane
B. setting up microtubule extensions that vesicles can follow in the movement of cytoplasm
C. reinforcing the pseudopod with intermediate filaments
D. none of the above are plausible answers.
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Spring 2011 Bio 1100 Sec03 Quiz 5 Key (blackboard quiz 3)

  1. Suppose that the plasma membrane around a flagellum is opened to reveal the axoneme inside. The dynein sidearms between the peripheral microtubule doublets are then removed by chemical treatment. ATP is then added. What is the expected observation? A. The axoneme will elongate or grow in size. B. There will be normal bending of the flagellum. C. There will be no movement, since the plasma membrane is not present. D. There will be no movement, because the ability to use ATP is lost.
  2. What can you infer about high-molecular-weight proteins that cannot be transported into the nucleus? A. They are foreign proteins. B. They lack nuclear localization signals (NLS). C. They are defective proteins. D. They have been tagged for destruction.
  3. What is the function of the nuclear pore complex? A. It regulates movement of materials across the nuclear envelope. B. It assembles ribosomes from raw materials that are synthesized in the nucleus. C. It synthesizes components of the endomembrane system. D. It synthesizes and repairs DNA.
  4. Scientists found that polypeptides that are normally synthesized in the endoplasmic reticulum are about 20 amino acids longer when they are synthesized by ribosomes not attached to the endoplasmic reticulum. What is/are possible explanations for the greater length of these polypeptides? A. The ribosomes that function as free ribosomes function differently than the ribosomes that are attached to the endoplasmic reticulum. B. The 20 amino acids serve as a signal sequence that directs the forming polypeptide to the endoplasmic reticulum, where they are cleaved off during processing. C. The 20-amino-acid sequence helps the endoplasmic reticulum package these proteins for shipping to the Golgi. D. The protein has a different function in the cytosol than in the endoplasmic reticulum.
  5. Researchers tried to explain how vesicular transport occurs in cells by attempting to assemble the transport components. They set up microtubular tracks along which vesicles could be transported, and they added vesicles and ATP (because they knew the transport process requires energy). Yet, when they put everything together, there was no movement or transport of vesicles. What were they missing? A. an axon B. contractile filaments C. motor proteins D. rough endoplasmic reticulum
  6. Amoeba move by crawling over a surface (amoeboid movement). Which one of the following processes is part of amoeboid movement? A. polymerization or extension of actin filaments to form pseudopodia or “false feet” in the plasma membrane B. setting up microtubule extensions that vesicles can follow in the movement of cytoplasm C. reinforcing the pseudopod with intermediate filaments D. none of the above are plausible answers.
  1. Which of the following cytoskeletal proteins are important in changing cell shape or location (any type of cell movement)? A. Actin is the only cytoskeletal proteins important in cell movement. B. Actin and myosin are among the cytoskeletal proteins important in cell movement. C. Actin, microtubules, and intermediate filaments are equally important in cell movement. D. Intermediate filaments are the only cytoskeletal proteins important in cell movement.
  2. What is the function of keratin, an important intermediate filament? A. imparts impermeability to the skin and the ability to withstand mechanical stress B. reinforces moveable joints C. enables the heart to contract as a unit D. provides nutrients to neural cells of the brain and spinal cord
  3. A protein lacking a signal sequence directing it to a particular location or organelle in a cell will likely reside _____. A. in the rough ER B. in the Golgi apparatus C. in the plasma membrane as an integral membrane protein D. in the cytoplasm
  4. A large carbohydrate is tagged with a fluorescent marker and placed in the extracellular environment surrounding a eukaryotic cell. The cell ingests the carbohydrate via endocytosis. Upon viewing the cells with a microscope, which of the following cellular substructures is likely to be fluorescently labeled? A. lysosomes B. mitochondria C. nucleus D. ribosomes
  5. In the pulse-chase experiment discussed in class, researchers applied a short pulse of radioactively labeled leucine, followed by a long chase of unlabeled leucine, to cells that secrete digestive enzymes. The bulk of the radioactive label was initially found in the rough ER, then in the Golgi apparatus, followed by secretory vesicles that emptied into a duct outside the cell. These results BEST support which of the following hypotheses? A. Materials exit the cell via endocytosis and enter via exocytosis B. Microtubules and other elements of the cellular cytoskeleton are dynamic C. Cargo is transported through the endomembrane system according to specific lipid “tags” D. Proteins that are destined to be secreted outside the cell follow a specific pathway that culminates with secretory vesicle fusion with the plasma membrane releasing the protein into the extracellular space.
  6. Counterclockwise rotation of prokaryotic flagella in response to an attractant will result in a___________. A. run B. tumble C. chemotaxis response D. both A and C E. both B and C