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Qualitative Test for Protein and Carbohydrates-Biotechnology and Bioengineering-Project Report, Study Guides, Projects, Research of Biotechnology

Sri Ramachandra Medical College and Research InstituteBiotechnology

This project report related to Biotechnology course and it was submitted to Prof. Rahul Khanna at Sri Ramachandra Medical College and Research Institute. It includes: Qualitative, Test, Proteins, Carbohydrates, Classification, Structure, Xanthprotic, Million, Molisch, Fehling

Typology: Study Guides, Projects, Research

2011/2012

Uploaded on 07/12/2012

kamin
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A
PROJECT REPORT
ON
“QUALITATIVE TEST FOR PROTEIN
AND
CARBOHYDRATES”
ACKNOWLEDGMENT
I wish to express my gratitude towards all those who have been helpful
to me in getting this mightily task of project done up.
I would like to acknowledge the overweening support, from both teacher
and student alike, enjoyed by biochemistry this gave me the confidence
& courage to effect on the project work.
Docsity.com
pf3
pf4
pf5

Partial preview of the text

Download Qualitative Test for Protein and Carbohydrates-Biotechnology and Bioengineering-Project Report and more Study Guides, Projects, Research Biotechnology in PDF only on Docsity!

A

PROJECT REPORT

ON

“ QUALITATIVE TEST FOR PROTEIN

AND

CARBOHYDRATES”

ACKNOWLEDGMENT

I wish to express my gratitude towards all those who have been helpful to me in getting this mightily task of project done up. I would like to acknowledge the overweening support, from both teacher and student alike, enjoyed by biochemistry this gave me the confidence & courage to effect on the project work.

I would like to express my appreciation for the generous support provided by Mrs. Sheetal Sharma and Mrs. Jagriti from their valuable help guidance during the course of project work.

POOJA

CONTENTS

A. Qualitative test for protein

  1. Nin hydrin test
  2. Xanthprotic test
  3. Million’s test
  4. Biuret test
  5. Pauly’s test B. Classification of Proteins C. Structure of Proteins D. Qualitative test for carbohydrates
  6. Molisch’s test
  7. Fehling test
  8. Benedict’s test
  9. Barfoed’s test
  10. Saliwanoff’s test
  11. Jodide test

Qualitative test for carbotiydrates.

  1. Molisch’s Test  2 ml of sample +2 drops of molisch’s reagent + 1 ml conc. H 2 So 4 along sides of test tube. Observation – Purple ring at junction of two layers. Principle – It is general test for all carbohydrates. Conc. H 2 So 4 hydrolysis glycosidic bond to yield monosaccharides that in the presence of acid get dehydrated to form furfural or its derivatives. Furfural derivatives reacts with sulphonated -napthol sol, (5% W/V in ethanol = prepared fresh as it is unstable) to form purple complex.
  2. Fehling’S Test  1 ml of sample + 1 ml of fehling’s A sol. + 1 ml of fehling’s B sol. -Boil. Observation – Brick-red PPt. Principle – This test is for reducing sugars i.e. sugar containing free aldehyde or ketonic group. Fehling’s A – CuSo 4 sol. Fehling’s B – Sol. Of KOH + Na-K tartarate(-rochelle’s salt) Reducing sugar reduce CuO (+2) of fehling’s reagent to Cu 2 O(+1) which forms red PPt. The free =C=O group of sugar is oxidized to -CooH group. Rochelle’s salt act as chelating agent.

complex prolonged heating will hydrochloride disoaccharides and other monosaccharides will eventually give colour.

  1. Jodide test  1ml sample + 5 drops of I 2 sol (in KI) Observation – Violet colour. Principle – I 2 forms coloured adsorption complex with polysaccharides. Starch gives blue colour while glycogen given reddish brown colour.