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Understanding Open Reading Frames and Protein Coding Regions in Nucleic Acids, Slides of Biology
The process of translating nucleic acid sequences into hypothetical protein sequences using the genetic code. It explains how translation can occur in forward and reverse reading frames, and introduces open reading frames (orfs) as regions that can be translated without encountering a stop codon. The document also covers methods for estimating the likelihood that a particular orf is a coding region, such as third-codon base composition and codon preference tables.
Typology: Slides
2011/2012
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Summary, Part 4
Translation of nucleic acid sequences into hypothetical protein sequences requires a genetic code
Translation can occur in three forward and three reverse reading frames
Open reading frames are regions that can be translated without encountering a stop codon
Summary, Part 4
The likelihood that a particular open reading frames is in fact a coding region (actually made into protein) can be estimated using third-codon base composition or codon preference tables
This can be used to scan long sequences for possible coding regions