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Portage Microbiology Exam 4 With Correct Questions and Answers., Exams of Microbiology

DeVry University - ArizonaMicrobiology

True or False: Growth media is best suited for distinguishing between two similar species of bacteria - A researcher is asked to determine which of two vials contains e coli and which contains salmonella. Knowing both gram-negative while only one of them is capable of fermenting lactose, which type of media would be best suited (multiple choice)

Typology: Exams

2023/2024

Available from 03/26/2024

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Portage Microbiology Exam 4 With
Correct Questions and Answers.
True or False: Growth media is best suited for distinguishing between two similar
species of bacteria - Correct Answer False. Growth media is designed to simply support
(and not restrict) microbial growth
A researcher is asked to determine which of two vials contains e coli and which contains
salmonella. Knowing both gram-negative while only one of them is capable of
fermenting lactose, which type of media would be best suited (multiple choice) - Correct
Answer Differential media distinguishes between two, often related, microbes
A researcher is asked to determine if a sample contains Neisseria meningitides.
Knowing Neisseria meningitides is slow growing and other foreign microbes may also
be present in the culture, which type of media would be best suited? (multiple choice) -
Correct Answer Selective media
What are the requirements of a fastidious microbe? (short answer) - Correct Answer A
fastidious microbe is an organism with complex growth requirements such that is absent
it will not grow. Enriched medias thus contain these specific and essential nutrients
required for the growth of a particular subset of microorganisms.
True or False: LB agar is classified as a selective, non-differential media - Correct
Answer False
What is agar used for in microbiology? (short answer) - Correct Answer Agar is used to
create a solid, smooth surface on which microbes can grow
Alpha hemolysis (matching) - Correct Answer Incomplete hemolytic activity
Beta hemolysis (matching) - Correct Answer Complete hemolytic activity
Gamma hemolysis (matching) - Correct Answer no hemolytic activity
Columbia CNA agar is most closely related to which media (multiple choice) - Correct
Answer blood agar
True or False: Chocolate agar gets its brown color from cocoa to produce an enriched
media - Correct Answer false
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Portage Microbiology Exam 4 With

Correct Questions and Answers.

True or False: Growth media is best suited for distinguishing between two similar species of bacteria - Correct Answer False. Growth media is designed to simply support (and not restrict) microbial growth A researcher is asked to determine which of two vials contains e coli and which contains salmonella. Knowing both gram-negative while only one of them is capable of fermenting lactose, which type of media would be best suited (multiple choice) - Correct Answer Differential media distinguishes between two, often related, microbes A researcher is asked to determine if a sample contains Neisseria meningitides. Knowing Neisseria meningitides is slow growing and other foreign microbes may also be present in the culture, which type of media would be best suited? (multiple choice) - Correct Answer Selective media What are the requirements of a fastidious microbe? (short answer) - Correct Answer A fastidious microbe is an organism with complex growth requirements such that is absent it will not grow. Enriched medias thus contain these specific and essential nutrients required for the growth of a particular subset of microorganisms. True or False: LB agar is classified as a selective, non-differential media - Correct Answer False What is agar used for in microbiology? (short answer) - Correct Answer Agar is used to create a solid, smooth surface on which microbes can grow Alpha hemolysis (matching) - Correct Answer Incomplete hemolytic activity Beta hemolysis (matching) - Correct Answer Complete hemolytic activity Gamma hemolysis (matching) - Correct Answer no hemolytic activity Columbia CNA agar is most closely related to which media (multiple choice) - Correct Answer blood agar True or False: Chocolate agar gets its brown color from cocoa to produce an enriched media - Correct Answer false

True or False: Chocolate is not a component of chocolate agar plates - Correct Answer true A researcher is studying a strain of e coli currently growing on a MacConkey plate. However, the researcher can't remember if e coli is gram positive or gram negative. Would a gram stain be necessary to confirm? Why or why not? (short answer) - Correct Answer No. A gram stain would not be necessary, as only gram negative microbes will grown on MacConkey agar. Thus, e coli is a gram negative microbe In an attempt to detect the presence of the pathogenic strain of e coli O157:H7, a researcher spread culture onto a MacConkey agar with failed results. What type of agar should they (correctly) try next? Why? (short answer) - Correct Answer The microbe should be plated on SMAC as it is specifically formulated to detect O157:H7. Pathogenic e coli cannot ferment sorbitol while non-pathogenic e coli can ferment both sorbitol and lactose. Therefore, colonies that ferment can be differentiated from non- fermenters. What is the gram status of microbes growing on eosin methylene blue agar plates? (short answer) - Correct Answer gram negative What is the process of spreading a bacterial culture onto a petri dish? (short answer) - Correct Answer plating Mannitol salt agar will turn what color in the presence of the pathogenic strain staphylococcus aureus? (short answer) - Correct Answer Yellow In order to visual individual colonies of bacteria, would you culture your sample in a liquid media or on a solid (agar) media? Why? (short answer) - Correct Answer Solid media. The primary advantage is that cells are held into place. When grown in a nutrient broth, bacterial cells can multiply but are free to move around in solution. When grown on agar within a petri dish the fixed in such a way as to form colonies True or False: the visualization of colonies on a petri dish represents bacterial cells that have often multiplied a million times over - Correct Answer True True or False: the purpose of a quadrant streak expand a bacterial population - Correct Answer False To be considered a pure culture, the sample can be traced back to a single cell and _________ - Correct Answer The culture must also be free from external contaminants. Would not contain multiple bacterial species When performing a quadrant streak, the sample is spread across the plate in such as way as to form what? (short answer) - Correct Answer the sample is spread into four regions where each new region is perpendicular to the previous region, such that each region will become more diluted than the previous.

What is the primary purpose of Columbia CNA agar? - Correct Answer Columbia CNA agar is an enriched, selective, and differential media. It suppresses the growth of Gram- negative bacteria and is, therefore, used for isolation of Gram-positive microbes. It also allows for differentiation based on hemolytic patterns.