Name:
Date:
Experiment #:3
Title:
Extraction (isolation of caffeine
from tea bags)
Purpose:
The goal of the experiment is to learn how to use extraction to purify compounds. The purpose
is to extract and isolate caffeine from tea bags.
Procedure:
1. Poured 15 mL water in beaker and placed it on hot plate.
2. Added small amount of sodium carbonate to neutralize the acidity of tea components.
3. Once the solution began to boil, added tea bag in beaker and heated for about 5 minutes.
4. Added small amount of boiling water to keep the tea bag submerged in solution.
5. Took out the tea bag and squeezed the liquid carefully.
6. Placed 2nd tea bag in solution and repeated the process.
7. Transferred the tea solution in centrifuge tube and placed it in ice bath.
8. Placed the tube on stand and added 2 mL dichloromethane solution in it.
9. Placed the cap on tube and shook it gently.
10. Placed it back in holder and allowed layers to settle.
11. Placed tube in centrifuge to break the emulsion at 50,000 RPM for 3 minutes.
