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Great photosynthesis lab report
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Title: Investigate the effects of sodium bicarbonate and light intensity on the rate of photosynthesis of green spinach leaves through the observation of floating leaf disks
Background information: When light is absorbed by leaf pigments such as chlorophyll a or b, electrons within each photosystem are boosted to a higher energy level. The leaves then produce ATP, to reduce NADP to NADPH, and incorporate CO 2 into organic molecules in a process called carbon fixation. When leaves are put in the water and undergo the process of the light-dependent reaction, oxygen is produced and released into the interior of the leaf which causes them to rise. However, cellular respiration is also occurring at the same time, consuming the oxygen produced in photosynthesis. The leaves must have a carbon source provided to them to allow photosynthesis to carry on. The amount of light the plant receives coupled with the amount of available CO 2 will influence the rate at which photosynthesis occurs. The aim of this experiment is to measure the effect of light intensity and availability of carbon and their effects of the rate of photosynthesis.
Hypothesis If the leaf is exposed to higher intensity of light in the presence of a carbon source, then it takes less time for the leaf disks to float to the surface of the solution due to an increased rate of O 2 production through photosynthesis.
Variables Control variables Independent Variables Dependent Variables Temperature Intensity of light Time it takes for disks to float Volume of infiltration solution Species of spinach Presence of CO 2 Size of leaf discs
Results
Raw data collection
Time (min) Number of floating disk Trial 1 20 cm CO₂ Light
Trial 2 10 cm CO₂ Light
Trial 3 Water/soap 10 cm
Trial 4 10cm / CO 2 Dark 1 0 1 0 1 2 0 1 0 0 3 0 1 0 0 4 0 2 0 0 5 0 2 0 0 6 0 2 0 0 7 0 1 0 0 8 0 2 0 0 9 0 2 0 0 10 0 2 0 0 11 0 2 0 1 12 0 1 0 2 13 0 1 0 3 14 0 2 0 4 15 0 3 0 5 16 1 4 0 5 17 2 4 0 5 18 2 5 0 4 19 2 6 0 4 20 2 7 0 3 21 2 7 0 2 22 2 9 0 2 23 2 9 0 2 24 2 10 0 2 25 2 10 0 1 26 2 10 0 1 27 2 10 1 0 28 2 10 1 0 29 2 10 1 0 30 1 10 1 0