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A lab that tests the effect of denaturing enzymes on peroxidase catabolism.
Typology: Summaries
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Name: ____________________________________________ Period: _____
LIVER ENZYME LAB The objectives for this lab are for students to:
demonstrate the function of an enzyme observe how a change in conditions can affect enzyme function
Background: All living cells contain enzymes. One of these enzymes, called catalase, breaks down a substance called hydrogen peroxide (𝐻 2 𝑂 2 ) that can build up in your cells. The enzymes change hydrogen peroxide into oxygen gas (𝑂 2 ) and water (𝐻 2 O). The chemical equation that represents this chemical reaction is: Enzyme (catalase) action 2 𝐻 2 𝑂 2 ---------------------------------------→ 𝑂 2 (gas) + 2𝐻 2 O
PROBLEM: What is the effect of high temperature on enzyme activity? HYPOTHESIS: (remember to state this as an IF……..Then….. Because…………..statement) If you apply high temperature to an enzyme Then______________________________________________ Because
EXPERIMENT:
MATERIALS: Each group of students will need to collect the following materials before beginning the lab: A paper plate This direction/lab report – one for each student 3 small cups A small forceps One graduated cylinder filled with 5ml of hydrogen peroxide One piece of raw chicken liver, one piece of cooked chicken liver Choose one piece of the following foods: apple, raw potato or spinach leaf
PROCEDURE:
One piece of frozen, raw, and cooked potato
, one piece of frozen chicken liver
Directions: Record the observations and measurements from your experiment. Share your data for the food you chose (apple, potato or spinach) with the other groups. Make sure that you have data for every different food used in this experiment.
Food source Bubble Amount ( 0 – 3 ) What is the enzyme in this experiment?
What is/are the products?
What is the substrate?
Raw liver
Cooked liver
Apple
Potato
Spinach leaf
CLEAN UP! Empty all cups into the trash can. DO NOT empty into any sink, it will clog. Throw away the raw liver cup and wash out the other 2 cups with soap and let dry on the mat by the sink. Return the paper plate, the forceps and the graduated cylinder to the front of the room.
ANALYSIS:
Frozen liver
Potato
Cooked Potato Frozen Potato