Docsity
Docsity

Prepare for your exams
Prepare for your exams

Study with the several resources on Docsity


Earn points to download
Earn points to download

Earn points by helping other students or get them with a premium plan


Guidelines and tips
Guidelines and tips

Peroxidase Lab for Anatomy, Summaries of Biology

A lab that tests the effect of denaturing enzymes on peroxidase catabolism.

Typology: Summaries

2023/2024

Uploaded on 10/23/2024

douglas-dalessandro
douglas-dalessandro 🇺🇸

1 document

1 / 2

Toggle sidebar

This page cannot be seen from the preview

Don't miss anything!

bg1
Name: ____________________________________________ Period: _____
LIVER ENZYME LAB
The objectives for this lab are for students to:
demonstrate the function of an enzyme
observe how a change in conditions can affect enzyme function
Background: All living cells contain enzymes. One of these enzymes, called catalase, breaks down a substance
called hydrogen peroxide (𝐻2𝑂2) that can build up in your cells. The enzymes change hydrogen peroxide into
oxygen gas (𝑂2) and water (𝐻2O). The chemical equation that represents this chemical reaction is:
Enzyme (catalase) action
2 𝐻2𝑂2 ---------------------------------------𝑂2 (gas) + 2𝐻2O
PROBLEM: What is the effect of high temperature on enzyme activity?
HYPOTHESIS: (remember to state this as an IF……..Then….. Because…………..statement)
If you apply high temperature to an enzyme Then______________________________________________
Because
EXPERIMENT:
1. What is the control group? ______________________________________
2. What is the independent variable? ________________________________
3. What is the dependent variable? _________________________________
MATERIALS:
Each group of students will need to collect the following materials before beginning the lab:
A paper plate
This direction/lab report one for each student
3 small cups
A small forceps
One graduated cylinder filled with 5ml of hydrogen peroxide
One piece of raw chicken liver, one piece of cooked chicken liver
Choose one piece of the following foods: apple, raw potato or spinach leaf
PROCEDURE:
1. Keep all materials on the blue tray
2. Number the cups 1 3
3. Add raw chicken liver to cup #1, cooked liver to cup #2 and your other food in cup #3
4. Get 5ml. of hydrogen peroxide (you will repeat this step 2 more times)
5. Add the 5ml. of hydrogen peroxide to cup #1 with the raw chicken liver
6. IMMEDIATELY observe the amount of bubbling (gas) in each cup. Use this scale:
0= no bubbles at all
1= small amount of bubbles
2= medium amount of bubbles (bubbling half way up the cup)
3=large amount of bubbles (bubbling more than half way up the cup)
7. Repeat steps # 4 - #6 with the cups #2 and #3
8. Record observations in data table
One piece of frozen, raw, and cooked potato
, one piece of frozen chicken liver
pf2

Partial preview of the text

Download Peroxidase Lab for Anatomy and more Summaries Biology in PDF only on Docsity!

Name: ____________________________________________ Period: _____

LIVER ENZYME LAB The objectives for this lab are for students to:

 demonstrate the function of an enzyme  observe how a change in conditions can affect enzyme function

Background: All living cells contain enzymes. One of these enzymes, called catalase, breaks down a substance called hydrogen peroxide (𝐻 2 𝑂 2 ) that can build up in your cells. The enzymes change hydrogen peroxide into oxygen gas (𝑂 2 ) and water (𝐻 2 O). The chemical equation that represents this chemical reaction is: Enzyme (catalase) action 2 𝐻 2 𝑂 2 ---------------------------------------→ 𝑂 2 (gas) + 2𝐻 2 O

PROBLEM: What is the effect of high temperature on enzyme activity? HYPOTHESIS: (remember to state this as an IF……..Then….. Because…………..statement) If you apply high temperature to an enzyme Then______________________________________________ Because

EXPERIMENT:

  1. What is the control group? ______________________________________
  2. What is the independent variable? ________________________________
  3. What is the dependent variable? _________________________________

MATERIALS: Each group of students will need to collect the following materials before beginning the lab:  A paper plate  This direction/lab report – one for each student  3 small cups  A small forceps  One graduated cylinder filled with 5ml of hydrogen peroxide  One piece of raw chicken liver, one piece of cooked chicken liver  Choose one piece of the following foods: apple, raw potato or spinach leaf

PROCEDURE:

  1. Keep all materials on the blue tray
  2. Number the cups 1 – 3
  3. Add raw chicken liver to cup #1, cooked liver to cup #2 and your other food in cup #
  4. Get 5ml. of hydrogen peroxide (you will repeat this step 2 more times)
  5. Add the 5ml. of hydrogen peroxide to cup #1 – with the raw chicken liver
  6. IMMEDIATELY observe the amount of bubbling (gas) in each cup. Use this scale: 0= no bubbles at all 1= small amount of bubbles 2= medium amount of bubbles (bubbling half way up the cup) 3=large amount of bubbles (bubbling more than half way up the cup)
  7. Repeat steps # 4 - #6 with the cups #2 and #
  8. Record observations in data table

One piece of frozen, raw, and cooked potato

, one piece of frozen chicken liver

DATA TABLE:

Directions: Record the observations and measurements from your experiment. Share your data for the food you chose (apple, potato or spinach) with the other groups. Make sure that you have data for every different food used in this experiment.

Food source Bubble Amount ( 0 – 3 ) What is the enzyme in this experiment?

What is/are the products?

What is the substrate?

Raw liver

Cooked liver

Apple

Potato

Spinach leaf

NEXT STEP:

CLEAN UP! Empty all cups into the trash can. DO NOT empty into any sink, it will clog. Throw away the raw liver cup and wash out the other 2 cups with soap and let dry on the mat by the sink. Return the paper plate, the forceps and the graduated cylinder to the front of the room.

ANALYSIS:

  1. What claim did your hypothesis make and what evidence do you have to support your claim? If you cannot support your hypothesis with evidence then what claim can you make with the evidence you have?
  2. Use your data table: What is your evidence that supports this claim? Claim: Heat affects enzyme function. Evidence:
  3. What other conditions, besides heat, can affect enzyme activity?
  4. Draw a model (Include the seen and unseen aspects of the experiment) of what happened in this experiment making sure you label every part. (Hint: We did this already!)

Frozen liver

Potato

Cooked Potato Frozen Potato