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This project report related to Biotechnology course and it was submitted to Prof. Rahul Khanna at Sri Ramachandra Medical College and Research Institute. It includes: Pathology, Lab, Isolation, Staphylococcus, Skin, Culturing, Pure, Broth, Filter, Paper, Disc
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1.^ ^ LB Media + AmpicillinAgent required (Stock Preparations)Requirement 20g/1 LB Broth 15/I, L gar 1 ml ampicillin solution 2. Nutrient broth Peptone Beef extract - - 5g3g Nacl Distilled water Ph. = 6.4 – 6.8 - - 1g 11 3. Preparation of Miniprep solution (Stock) i. Solution – 9g glucose , 2.5ml IM tris, 2ml 0.5media 100ml distilled water (GTE) ii. iii. Solution II – 2nml 10% SDS, 1ml 4N NaohSolution III. – 60ml 5M CH distilled water. 3 CooR, 11.5ml glacial gcetic acid, 25.5ml **4. 5. 6. 95% Ethanol70% EhanolTE Buffer (Tris EDTA)
Contents AIMIntroduction Isolation ofSub culturing to obtain pure broth cultures Staphylococcus from Skin Filter paper Disc methodChemical composition & mode of action of
different soaps & Tooth PastesBibliography