Docsity
Log inSign up
Docsity
Prepare for your exams
Prepare for your exams

Study with the several resources on Docsity

Earn points to download
Earn points to download

Earn points by helping other students or get them with a premium plan

Guidelines and tips
Guidelines and tips
Sell on Docsity
Sell on Docsity
Log inSign up

Prepare for your exams

Study with the several resources on Docsity

Find documents

Prepare for your exams with the study notes shared by other students like you on Docsity

Search Store documents

The best documents sold by students who completed their studies

Search through all study resources
Docsity AINEW

Summarize your documents, ask them questions, convert them into quizzes and concept maps

Explore questions

Clear up your doubts by reading the answers to questions asked by your fellow students

Earn points to download

Earn points by helping other students or get them with a premium plan

Share documents
download point icon20 Points

For each uploaded document

Answer questions
download point icon5 Points

For each given answer (max 1 per day)

All the ways to get free points
Get points immediately

Choose a premium plan with all the points you need

Study Opportunities

Choose your next study program

Get in touch with the best universities in the world. Search through thousands of universities and official partners

Community

Ask the community

Ask the community for help and clear up your study doubts

University Rankings

Discover the best universities in your country according to Docsity users

Free resources

Our save-the-student-ebooks!

Download our free guides on studying techniques, anxiety management strategies, and thesis advice from Docsity tutors

From our blog

Exams and Study
Go to the blog

UMass Amherst Biochem Teaching Initiative: Noncovalent Interactions & Acid/Base Chem - Pro, Study notes of Chemistry

University of Massachusetts - AmherstChemistry
Prof. Lynmarie Thompson

An outline from a biochemistry teaching initiative at umass amherst covering noncovalent interactions that stabilize the dna double helix and the significance of acid/base chemistry. Topics include electrostatic interactions, hydrogen bonds, van der waals forces, hydrophobic interactions, and the hydrophobic effect. The document also discusses the importance of these interactions to the stability of dna and proteins.

Typology: Study notes

Pre 2010

Uploaded on 09/17/2009

koofers-user-fa8
koofers-user-fa8 🇺🇸

10 documents

1 / 20

Toggle sidebar

This page cannot be seen from the preview

Don't miss anything!

bg1
UMass Amherst Biochemistry Teaching Initiative
...
Outline Fri 1/30/09
Noncovalent Interactions (Ch 1)
What stabilizes the DNA double helix?
Covalent bonds determine sequence
Noncovalent interactions much weaker
Electrostatic -- phosphates
Effect of dielectric
Hydrogen bonds -- base pairs
Distance & geometry
Competition with water
Van der Waals -- base stacking
Hydrophobic interactions -- base stacking
Entropic effect
Reasoning about importance to stability
Reminder: all this happens in the cell… !
pf3
pf4
pf5
pf8
pf9
pfa
pfd
pfe
pff
pf12
pf13
pf14

Partial preview of the text

Download UMass Amherst Biochem Teaching Initiative: Noncovalent Interactions & Acid/Base Chem - Pro and more Study notes Chemistry in PDF only on Docsity!

UMass Amherst Biochemistry Teaching Initiative 



...

Outline

Fri 1/30/

Noncovalent Interactions

(Ch 1)

Noncovalent interactions much weakerCovalent bonds determine sequence What stabilizes the DNA double helix?

Electrostatic -- phosphates

Effect of dielectric

Hydrogen bonds -- base pairs

Competition with waterDistance & geometry

Hydrophobic interactions -- base stackingVan der Waals -- base stacking

Entropic effect

Reasoning about importance to stability

Reminder: all this happens in the cell…!





...

Chemical properties determine function: DNA example

4 bases form 2 base pairs via H bonds

Same size/shape -A-T & G-C

fi

t into double helix

Unique base-pairing makes copying possibleSequence encodes information

 Today:

Noncovalent interactions (eg H bonds) -

Makes assembly/disassembly possible in mild conditionsWeaker than covalent interactions

eg for copying DNA

Many together stabilize unique 3D structures

eg DNA double helix

Acid/base chemistry





...

Covalent bonds





...

Noncovalent interactions

Horton

UMass Amherst Biochemistry Teaching Initiative 



...





...

H

O

H





...





...

Hydrophobic interactions Hydrophobic effect

NOT a hydrophobic “bond”

amphipathic = amphiphilicHydrophobicHydrophilic

examples:

lipiddetergentsurfactant





...





...





...

UMass Amherst Biochemistry Teaching Initiative 



... Outline Fri 1/30/

Acid/base chemistry of ionizable groups

(Ch 1-2)

Signi

fi

cance of pKa - deducing species present

Buffer solutionspH extremes disrupt structures of DNA, proteinsHenderson-Hasselbach equation





...





...