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Typology: Exercises
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Mutations Worksheet - Deletion, Insertion & Substitution There are several types of mutations :
--- Deletion & insertion may cause what’s called a FRAMESHIFT mutation, meaning the reading “frame" changes, thus changing the amino acid sequence from this point forward
--- If a substitution changes the amino acid, it’s called a MISSENSE mutation --- If a substitution does not change the amino acid, it’s called a SILENT mutation --- If a substitution changes the amino acid to a “stop , ” it’s called a NONSENSE mutation Complete the boxes below. Classify each as Deletion , Insertion or Substitution AND as either frameshift , missense , silent or nonsense ( Hint: Deletion & Insertion will always be frameshift). Original DNA Sequence: T A C A C C T T G G C G A C G A C T … mRNA Sequence: A U G / U G G / A A C / C G C / U G C / U G A Amino Acid Sequence: Methionine-Tryptophan-Asparagine-Arganine-Cysteine-Stop Mutated DNA Sequence #1 T A C A T C T T G G C G A C G A C T … What’s the mRNA sequence? A U G / U A G / A A C / C G C / U G C / U G A ( Circle the change ) What will be the amino acid sequence? Methionine-Stop Will there likely be effects? Yes! What type of mutation is this? Nonsense Mutated DNA Sequence #2 T A C G A C C T T G G C G A C G A C T … What’s the mRNA sequence? A U G / C U G / G A A / C C G / C U G / C U G / A ( Circle the change ) What will be the amino acid sequence? Methionine-Leucine-Glutamic Acid-Proline-Leucine-Leucine- Will there likely be effects? Yes! What type of mutation is this? Insertion Mutated DNA Sequence #3 T A C A C C T T A G C G A C G A C T … What’s the mRNA sequence? A U G / U G G / A A U / C G C / U G C / U G A ( Circle the change ) What will be the amino acid sequence? Methionine-Tryptophan-Asparagine-Arganine-Cysteine-Stop Will there likely be effects? No! What type of mutation is this? Silent Mutated DNA Sequence #4 T A C A C C T T G G C G A C T A C T … What’s the mRNA sequence? A U G / U G G / A A C / C G C / U G A / U G A ( Circle the change ) What will be the amino acid sequence? Methionine-Tryptophan-Asparagine-Arganine-Stop Will there likely be effects? Yes! What type of mutation is this? Nonsense
Original DNA Sequence: T A C A C C T T G G C G A C G A C T … mRNA Sequence: A U G / U G G / A A C / C G C / U G C / U G A Amino Acid Sequence: Methionine-Tryptophan-Asparagine-Arginine-Cysteine-Stop Mutated DNA Sequence #5 T A C A C C T T G G G A C G A C T … What’s the mRNA sequence? A U G / U G G / A A C / C C U / G C U / G A ( Circle the change ) What will be the amino acid sequence? Methionine-Tryptophan-Asparagine-Proline-Alanine- Will there likely be effects? Yes! What type of mutation is this? Deletion
Sickle cell hemoglobin AA sequence Valine-Histidine-Leucine-Threonine-Proline-Valine
4. What type of mutation is this? Please explain why. This is a substitution-missense mutation because one base changes and it changes the amino acid sequence.