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MLS ASCP Body Fluids Exam with Questions and Answers Urine handling ANSWER examine within 1 hour room temp and 2-4 hours refrigerated green/blue urine ANSWER indicate presence of biliverdin, medications containing dye, or pseudomonas infections Dark-brown/black urine ANSWER indicate presence of homogentisic acid (alkaptonuria) melanin Urine smells ANSWER Sulfur: asparagus metabolite Musty/mousy: PKU and some liver diseases Ammonia: old samples Sweet: maple syrup urine disease Pungent: may be associated with ketones Urine specific gravity methods of analysis ANSWER Refractometer: indirect measurement based on refractivity of light Dipstick: color change based on pKa change Urine pH ANSWER - Dipstick: orange = acid, blue = alkaline - Becomes more alkaline upon standing due to urease created by bacteria Glucose dipstick false positive ANSWER peroxide, bleach, or strong oxidize Clinitest tablets ANSWER Copper reduction test
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