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Microscale thermophoresis (MST) is a sensitive, high-throughput technique used to detect and characterize biomolecular interactions, including protein-DNA, protein-RNA, protein-protein, antigen-antibody, and ligand-ternary complex interactions. With its advantages of low sample consumption, short experiment time, and real-time affinity data acquisition, MST is suitable for high-throughput screening (HTS) and fragment-based screening (FBS) in drug discovery. Creative Biostructure offers reliable MST services for hit compound characterization, including determination of binding stoichiometries, thermodynamic parameters, and application of MST technology to HTS and FBS.
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Brief Introduction to Microscale Thermophoresis (MST)
MST is a non-immobilized technique for quantitative analysis of
biomolecular interactions in solution. The technique is based on
thermophoresis and fluorescence detection. The instrument of MST
utilizes an infrared laser for local heating to cause molecular directional
movement, and then analyze the molecular distribution ratio in the
temperature gradient field by fluorescence (fluorescence labeling or
intrinsic fluorescence). The MST technique can detect ligand binding-
caused changes in thermophoretic mobility, which rely on size, charge,
hydration shell, and conformations. Kd values can be estimated using
these changes in thermophoretic mobility.
Advantages of our MagHelix™ Microscale Thermophoresis (MST) services:
biomolecular interactions, and our MST services have been proven to be
reliable by customers from biotech, pharmaceutical, and biopharmaceutical
industries.
(Gibbs free energy), ΔG H (enthalpy), and ΔG S (entropy).
high-throughput screening of compounds/fragments and
fragment-based screening.
compensated to make the results more reliable.
As an advanced contract service provider in the drug discovery phase, Creative
Biostructure can simultaneously apply a variety of established approaches and
biophysical techniques to identify, validate, classify and characterize the hit
compounds according to the goals and requirements of the project. If your
project has such needs, please feel free to contact us, and our scientists will
provide professional consultation for you.
References
1.Alexander C G.; et al. Novel microscale approaches for easy, rapid determination of protein
stability in academic and commercial settings. Biochimica et Biophysica Acta (BBA)-Proteins and
Proteomics. 2014, 1844(12): 2241-2250.
2.Bartoschik T.; et al. Microscale thermophoresis in drug discovery. Applied Biophysics for Drug
Discovery. 2017: 73-99.
3.Rainard J M.; et al. Using microscale thermophoresis to characterize hits from high-
throughput screening: a European lead factory perspective. SLAS DISCOVERY: Advancing Life
Sciences R&D. 2018, 23(3): 225-241.