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Microbiology Theory_Microbial Growth, Study notes of Microbiology

The reason to study and understand microbial/bacterial growth is to know measure its growth like other living things, we can not measure way/method: -Restricted growth especially in hospital rooms, in setup facilities the amount, the number of cells can be counted, the bio mass can be measured and the weight can be calculated. food (kitchen, restaurant, etc); treat infectious diseases; etc. -Promote growth, clinical specimens can be made a diagnosis of disease; industry brewing (brewery); pharma

Typology: Study notes

2020/2021

Available from 12/16/2022

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brewing (brewery); pharmaceutical industry (antibiotic drugs); etc.
a population then must continue to divide must still exist. Bacterial cells will not exist forever
Meeting 6
How to measure bacterial growth?
in the growing phase, it must divide.
Microbiology Theory
Which is used as a benchmark to measure the growth of bacteria that can be seen
Microbial Growth
by measuring the number of cells. Because bacteria are tiny, it's impossible to
The reason to study and understand microbial/bacterial growth is to know
measure its growth like other living things, we can not measure
way/method:
length height. So we can measure the growth of bacteria by counting
Restricted growth especially in hospital rooms, in setup facilities
the amount, the number of cells can be counted, the bio mass can be measured and the weight can be calculated.
food (kitchen, restaurant, etc); treat infectious diseases; etc.
The number of these cells continues to increase because they continue to divide, cell division is a process
Promote growth, clinical specimens can be made a diagnosis of disease; industry
main role in the life of the bacterial cell to maintain the existence of the inner bacterial cell
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brewing (brewery); pharmaceutical industry (antibiotic drugs); etc.

a population then must continue to divide must still exist. Bacterial cells will not exist forever

Meeting 6

How to measure bacterial growth?

in the growing phase, it must divide.

Microbiology Theory

Which is used as a benchmark to measure the growth of bacteria that can be seen

Microbial Growth

by measuring the number of cells. Because bacteria are tiny, it's impossible to

The reason to study and understand microbial/bacterial growth is to know

measure its growth like other living things, we can not measure

way/method:

length height. So we can measure the growth of bacteria by counting

  • Restricted growth especially in hospital rooms, in setup facilities the amount, the number of cells can be counted, the bio mass can be measured and the weight can be calculated.

food (kitchen, restaurant, etc); treat infectious diseases; etc.

The number of these cells continues to increase because they continue to divide, cell division is a process

  • Promote growth, clinical specimens can be made a diagnosis of disease; industry

main role in the life of the bacterial cell to maintain the existence of the inner bacterial cell

large: protists and yeast. There are 2 techniques, namely spread plate and pour plate. Actual number of cells Bacterial generation time is the time it takes for a cell to divide.

  • Flow cytometer -> counts red and white blood cells, microorganism cells miroorganism or bacteria was confirmed based on the number of colonies For example, one cell becomes two cells. Bacterial generation time is the time it takes bacteria large size -> widely used in hospital laboratory. formed because every single bacterial cell will surely grow and continue to divide to double the amount. Most bacteria go through binary fission, model
  • Polycarbonate membrane filter: aquatic bacteria can be counted directly by means form colonies, each one cell forms a colony. So the number of colonies that the cleavage is one lengthwise and finally it becomes two, the cleavage that is the product filtered -> paint the bacteria with fluorescence dye (eg acridine orange) or DNA calculated and then multiplied by the dilution. stain DAPI, and observed under a microscope or grown on the same medium same as its parent. However, there are also divisions that are not the same as the parent.
  • Measurement of cell mass/(Calculating cell mass): Example of the same division with its parent. suitably and incubated for 24 hours. Tools for counting microorganisms that are larger in size than bacteria: Counting the number of colonies/Calculating the number of cells:
  • Coulter counter -> electronic counter -> directly calculate the size of microorganisms
  • Indirect viable cell counts (plate counts)