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This document contains the answers to the first lab exam/ lab midterm. ALL CORRECT ANSWERS ARE HIGHLIGHTED. SOME QUESTIONS HAVE MORE THAN 1 ANSWER. QUESTIONS MAY BE SHUFFLED DUE TO THE PROFESSOR ON YOUR END.
Typology: Exams
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This is a multiple answer question. You must choose all that apply to receive full credit. This sample of unknown bacteria was stained using the Gram stain procedure. What do the results tell you about the bacteria? cocci in clusters Gram positive Gram negative cocci in chains rods in clusters
Immersion oil should be used with the 40x objective to minimize the loss of light, and help magnify the bacteria you are observing.
Which of the following structures helps a bacterium escape phagocytosis by white blood cells?
During the procedure of making a streak plate, the purpose of flaming the inoculating loop between streaks is? Sterilize the loop before going back into the original broth culture to get another loopful of bacteria to streak the next area on the plate Kill the bacteria on the loop resulting in fewer cells being moved across the agar into the next area
What additional step is required when preparing a bacterial smear from a solid culture versus a liquid culture? You must heat fix multiple times You must use a lot more bacteria You must use a different kind of stain You must place a drop of water on the slide
This is a multiple answer question. You must choose all correct answers to get full credit. What bacterial characteristics can you determine from the following image?
it has a capsule it contains an endospore the morphology is spirilli it has flagella
This is a multiple answer question. You must choose all that apply to receive full credit. This PEA agar plate was inoculated with an unknown bacteria, and incubated. What do the results tell you about the bacteria?
This is a multiple answer question! You must choose all the correct answers to get full credit This EMB agar plate was inoculated with an unknown bacteria, and incubated. What do the results tell you about the bacteria? Gram positive Is a probable coliform Gram negative Is a possible coliform
This is a multiple answer question! You must choose all the answers that are correct to receive full credit. A SIM media tube was inoculated with an unknown bacteria. The following image shows the result after incubation. What does this result tell you about the bacteria? the bacteria produces gelatinase the bacteria is possibly E. coli the bacteria is possibly P. vulgaris the bacteria produces hydrogen sulfide
This is a multiple answer question! You must choose all the answers that are correct to receive full credit. A gelatin tube was inoculated with an unknown bacteria. After incubation, the tube was put on ice for 30 minutes. The following image shows the result. What does this result tell you about the bacteria? the bacteria hydrolyzes gelatin
the bacteria produces gelatinase the bacteria cannot produce gelatinase the bacteria cannot hydrolyze gelatin
Which of the following bacterial species can produce the structure shown in the following image? Staphylococcus aureus Klebsiella pneumoniae Bacillus cerus Proteus vulgaris
This is a multiple answer question! You must choose all the correct answers to get full credit
This is a multiple answer question! You must choose all the correct answers to get full credit This EMB agar plate was inoculated with an unknown bacteria, and incubated. What do the results tell you about the bacteria? Inhibited by Eosin Y and Methylene blue Not inhibited by Eosin Y and Methylene blue Gram positive
Gram negative Is a non-coliform
This is a multiple answer question! You must choose all the answers that are correct to receive full credit. A Simmon's citrate agar slant was inoculated with an unknown bacteria. The following image shows the result after incubation. What does this result tell you about the bacteria? the bacteria can utilize citrate as a sole carbon source the bacteria is possibly Staphylococcus epidermidis the bacteria is possibly Bacillus cereus
A Simmon's citrate agar slant was inoculated with an unknown bacteria. The following image shows the result after incubation. What does this result tell you about the bacteria?
the bacteria cannot utilize citrate as a sole carbon source the bacteria does not produce citrate permease the bacteria produces hydrogen sulfide
This is a multiple answer question! You must choose all the correct answers to get full credit This MAC agar plate was inoculated with an unknown bacteria, and incubated. What do the results tell you about the bacteria?
A mannitol broth was inoculated with a unknown bacteria and incubated.
What is the total magnification of a microscope if the oculars are 10x and the 10x objective is in place?
This is a multiple answer question. You must choose all that apply to receive full credit. This sample of unknown bacteria was stained using the Gram stain procedure. What do the results tell you about the bacteria?