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MICROBIOLOGY MIDTERM LAB EXAM WITH CORRECT AND VERIFIED ANSWERS 2025, Exams of Nursing

New Jersey City University (NJCU)Nursing

MICROBIOLOGY MIDTERM LAB EXAM WITH CORRECT AND VERIFIED ANSWERS 2025

Typology: Exams

2024/2025

Available from 06/23/2025

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MICROBIOLOGY MIDTERM LAB EXAM WITH
CORRECT AND VERIFIED ANSWERS 2025
light microscope - correct answers magnify 1000x
live organisms
um (unit of measurement)
see cells and large organelles
dont have to kill, can see live organisms
electron microscope - correct answers see viruses and large molecules (DNA double
helix)
no use light, electrons are used to view
100,000x magnification
nm (unit of measurement)
only preserved organisms (kill and thinly slice)
2 types: scanning (see outside), transmission (see in side)
aseptic technique - correct answers sterile, free from contamination caused by
harmful bacteria, virus, or other microorganisms
objective lens - correct answers #1. 4x (40x): red; scanning lens
#2. (100x): yellow; low power
#3. 40x (400): blue; high power
#4. 100x (1000): white; oil immersion is used because of the light waves and it
diminishes refraction
refraction - correct answers is the bending of light
What are nosocomial infections? - correct answers nosocomial infections are obtained
in a healthcare setting.
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CORRECT AND VERIFIED ANSWERS 2025

light microscope - correct answers magnify 1000x live organisms um (unit of measurement) see cells and large organelles dont have to kill, can see live organisms electron microscope - correct answers see viruses and large molecules (DNA double helix) no use light, electrons are used to view 100,000x magnification nm (unit of measurement) only preserved organisms (kill and thinly slice) 2 types: scanning (see outside), transmission (see in side) aseptic technique - correct answers sterile, free from contamination caused by harmful bacteria, virus, or other microorganisms objective lens - correct answers #1. 4x (40x): red; scanning lens #2. (100x): yellow; low power #3. 40x (400): blue; high power #4. 100x (1000): white; oil immersion is used because of the light waves and it diminishes refraction refraction - correct answers is the bending of light What are nosocomial infections? - correct answers nosocomial infections are obtained in a healthcare setting.

CORRECT AND VERIFIED ANSWERS 2025

What are fomites? - correct answers fomites are inanimate objects that can transmit infectious agents. What microscope is used to view a virus? - correct answers An electron microscope is used to view viruses. what do light and oil have in common? - correct answers oil and light have similar refractory period what is an inoculation needle used for? - correct answers it is used to transfer bacteria from solid media what is an inoculation loop used for? - correct answers it is used to transfer bacteria from liquid media 3 types of media - correct answers 1. liquid media

  1. solid media
  2. semi-solid media media is the collection of media why is heat fixing important? - correct answers heat is fixing is important to kill the bacteria and it makes the bacteria stick to the slide. culture - correct answers is a collection of media and organisms liquid media - correct answers t can be contained in small tubes or large industrial vessels. the advantages are that it can grow a large number if cells. disadvantage is that it is hard to identify contamination. solid media - correct answers tested in plates or test tubes, which can be agar, slant, or deep. the advantage is that it is easy to spot contamination. the disadvantage is that you can only grow a small # of cells. colony - correct answers mass of bacterial cells that are descendants of 1 cell

CORRECT AND VERIFIED ANSWERS 2025

some filters are small enough to filter out viruses - correct answers True, some filters are small enough to filter out viruses How many endospores are there per bacterial cell? - correct answers 1, there is only 1 endospore for bacterial cell one reason for making a streak plate - correct answers one of the reasons to make a streak plate is to isolate colonies how many times does the inoculation loop come in contact with the original liquid bacterial culture when making a streak plate? - correct answers the inoculation loop comes in contact with the original liquid bacterial culture once (1x ) when making a streak plate what happens if you forget to cool the inoculation loop when doing a streak plate? - correct answers if you forget to cool the inoculation loop you kill the bacteria What structure is stained with copper sulfate? - correct answers the capsule is stained by copper sulfate endospores are reproductive structures - correct answers False, endospores do not reproduce virulence increases with increased thickness of a bacterial capsule - correct answers true, virulence increases with increased thickness of a bacterial capsule a bacteria can be virulent, but not pathogenic - correct answers streptococcus bacteria are able to form endospores - correct answers False, streptococcus bacteria cannot form endospores It is important to heat fix the bacterial smear when doing an Anthony's stain - correct answers False, heat fixing a bacterial smear is not important when doing an Anthony's stain cell arrangement - correct answers strepto- chain staph- cluster

CORRECT AND VERIFIED ANSWERS 2025

diplo- in pairs (2) tetrads- 4 cells arranged in a cube sarcinae- 8 cells arranges in a cube compound microscope - correct answers cell shape - correct answers coccus-round bacillus- rod like spirillum- sprial vibrio- coma shaped spirochete- corkscrew single staphylococcus gram + - correct answers spirillum always single - correct answers gram + streptobacillus - correct answers When a slide has a dark background and white highlighted bacteria, a negative stain was used. Possible dyes used could be nigrosin, eosin, and india ink. - correct answers What does it mean to be pathogenic? - correct answers it is the ability to cause disease What is virulence? - correct answers degree to which it causes diseases. if a bacteria has a thicker capsule layer than virulence is higher than non-capsulated bacteria.