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This document contains the answers to the final lab exam. ALL CORRECT ANSWERS ARE HIGHLIGHTED. THERE ARE MULTIPLE ANSWERS TO SOME QUESTIONS. QUESTIONS MAY BE SHUFFLED DUE TO THE PROFESSOR ON YOUR END.
Typology: Exams
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Question 1 1 / 1 point This is a multiple answer question! You must choose all the correct answers to get full credit. This EMB plate was inoculated with an unknown bacterium, and incubated. What do the results tell you about the bacterium? This unknown bacterium is a noncoliform The unknown bacterium not inhibited by bile salts The unknown bacterium is not inhibited by eosin Y and methylene blue This unknown bacterium is a probable coliform Question 2 0.5 / 1 point
This is a multiple answer question. You must choose all that apply to receive full credit. Which of the following bacteria should grow well on EMB agar? Bacillus Staphylococcu s Klebsiella Proteus Question 3 1 / 1 point The bacteria on this plate is S. aureus. S. aureus is more resistant to Antibiotic #1 than Antibiotic #2. True
940 ml 94 ml 94 μl 940 μl Question 6 1 / 1 point During the procedure of making a streak plate, the purpose of flaming the inoculating loop between streaks is? Kill the bacteria on the loop resulting in fewer cells being moved across the agar into the next area To practice aseptic technique Help the bacteria grow by warming them up Help the bacteria grow by warming them up Sterilize the loop before going back into the original broth culture to get another loopful of bacteria to streak the next area on the plate
Question 7 1 / 1 point You completed a Gram stain on unknown bacterial species, and also inoculated and incubated a Urea broth with the same unknown bacteria. The results were:
Tryptic soy agar Mueller Hinton agar Phenylethyl alcohol agar Antibiotic agar Question 12 0 / 1 point UV light can penetrate the clear plastic petri dish lid. True False Question 13 1 / 1 point You completed an endospore stain on unknown bacterial species, and also inoculated a Simmon's citrate agar slant with the same unknown bacteria and incubated it. The results were:
Bacillus subtilis Micrococcus luteus None of the above Question 14 1 / 1 point This is a multiple answer question. You must choose all that apply to receive full credit. This sample of unknown bacteria was stained using the Gram stain procedure. Which of the following are possible identifications of the genera of this bacteria? Salmonella Bacillus Staphylococcus
B. cereus has a capsule B. cereus forms endospores B. cereus has a flagella B. cereus has a waxy cell wall Question 17 1 / 1 point True/False: Enzymes can be denatured by boiling water. True False Question 18 1 / 1 point The image below shows the results of a "Control of Microbial Growth by Moist Heat" experiment.
A bacteria was subjected to the temperature below each image for 10 minutes, swabbed onto the agar plate and incubated to determine if the bacteria could survive that temperature. What is the Thermal Death Point (TDP) for this bacteria? 60°C 60°C - 100°C 100°C 37°C - 60°C Question 19 1 / 1 point The Use-dilution method for testing the effectiveness of disinfectants was used to test the effectiveness of hydrogen peroxide against K. pneumoniae. The image below shows the results. What structure produced by K. pneumoniae may have contributed to the result seen in the the tube in which the bead was treated by 1% hydrogen peroxide?
endospore no structure capsule thick cell wall Question 21 1 / 1 point If you plate 0.1 ml of a culture from a tube that has a tube dilution of 10-5^ what is the final dilution on the plate? 10 - 10 - 10 - TNTC Question 22 1 / 1 point
This is a multiple answer question. You must choose all that apply to receive full credit. The image below shows the results of a Sure-vue test. Based on the results, which of the following is true about the bacterium that was tested? it is possibly Staphylococcus epidermidis it can't produce coagulase it is possible Staphylococcus aureus it can produce coagulase Question 23 1 / 1 point This is a multiple answer question. You must choose all that apply to receive full credit. This SS agar plate was inoculated with an unknown bacterium. What does this result tell you about the bacterium?
True False Question 25 1 / 1 point What is the living, metabolizing part of the bacterial cell called that surrounds the endospore as it's forming? the free endospore the capsule the vegetative cell the duplicate cell Question 26 1 / 1 point You did a Novobiocin test on an unknown bacterium. The result was a zone of inhibition 13 mm in diameter. Which of the following is a possible identification of the unknown bacterium? Staphylococcus saprophyticus Staphylococcus aureus Staphylococcus epidermidis None of the above
Question 27 1 / 1 point What method was used to test the antibiotic sensitivity of bacteria? Antimicrobic method Use-dilution method Mueller Hinton method Kirby-Bauer method Question 28 1 / 1 point This is a multiple answer question. You must choose all that apply to receive full credit. A blood agar plate was inoculated with an unknown species of Gram-positive bacteria. What does this result tell you about the bacterium?
The results were:
the bacterium ferments mannitol the bacterium is inhibited by NaCl the bacterium is possibly Staphylococcus aureus the bacterium is possibly Staphylococcus epidermidis Question 32 0 / 1 point The bacteria on this plate is S. aureus. If you were going to prescribe one of these antibiotics for a S. aureus infection, which one would probably be more effective Antibiotic #1 or Antibiotic #2)? Antibiotic # Antibiotic # Neither antibiotic will be effective
