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General microbiology data sheets
Typology: Papers
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1. Identify the bacterial species using DNA identification. The bacterial species of sample D was Salmonella typhimurium. This was proven using DNA identification and the symptoms of the patient including fever, headache, joint pain, constipation, loss of appetite, and abdominal pain. 2. What is the gene used in identification? (1 google scholar reference). The gene used in identification of bacterial species is the 16S rRNA gene sequences. 3. Why do you think this gene is a good reference in identifying bacteria? The 16S rRNA is a good reference in identifying bacteria because of its presence in all bacteria. Also, the function of the 16S rRNA gene has not changed much over time. Lastly, the gene is large enough for data collection purposes. 4. DNA sequencing is somehow similar to a natural biological process. Mention the natural process and explain the similarities between sequencing and that natural process. (3 pts) DNA sequencing also known as the ‘Sanger Method’ is very similar to the natural process of DNA replication. DNA sequencing is the process of making many copies of a target DNA region which is the same as DNA replication. 5. Explain how DNA sequencing is different from the natural biological process mentioned in question 4. (3 pts) DNA sequencing and DNA replication are different because in DNA sequencing you look for an exact part of the DNA while in replication you copy the whole thing. Also, DNA sequencing uses chain terminator nucleotides. 6. What is PCR and how does it work? PCR stands for Polymerase Chain Reaction which is a way to create many copies of DNA. 7. What natural biological process is similar to PCR? The PCR process then uses these ingredients to mimic the natural DNA replication process that occurs in cells.
Clarridge III, Jill E. "Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases." Clinical microbiology reviews 17.4 (2004): 840-862.