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Lowry Protein Assay Protocol (from Scott Hsieh). Solution A: 4 mg/mL NaOH and 20 mg/mL Na2CO3 in water. Add 2 g of NaOH and 10 g of Na2CO3 to 400 mL water ...
Typology: Exercises
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y = 0.055x + 1E- R^2 = 1
y = 0.055x + 0. R^2 = 1
uL Sample added
Abs. Standard alone
Protein + standard
Linear (Standard alone) Linear (Protein + standard)
y = 0.055x + 1E- R^2 = 1
y = 0.0441x + 0. R^2 = 0.
uL Sample added
Abs.^ Standard alone
Protein + standard
Linear (Standard alone) Linear (Protein + standard)
μg protein added 0 2.5 5 7.5 10 12.5 15 17.5 20 25 30 35
2mg/mL BSA (μL) 0 1.25 2.5 3.75 5 6.25 7.5 8.75 10 12.5 15 17.
H 2 0 (μL) 200 198.75 197.5 196.25 195 193.75 192.5 191.25 190 187.5 185 182.