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This lab report outlines an experiment designed to investigate ocean acidity and its effects on shelled marine species. Students will explore the relationship between pH levels and carbonic acid formation, as well as the impact of varying pH solutions on different shell species. The report includes instructions for conducting the experiment, recording data, and analyzing results.
What you will learn
Typology: Exercises
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Introduction To investigate the principles of Oceanography and our Oregon coastal regions, you will complete a variety of exercises including answering questions based on diagrams, searching websites, and research current trends. You will also use data collected on our field trips to analyze our coastal oceanographic parameters. You will need access to a few materials so read ahead and make sure you have what you need.
The pH scale measure the acidity or alkalinity of a solution by measuring the concentration of hydrogen ions it contains. The more hydrogen ions there are, the more acidic the solution. Refer to Figure 5.20 on page 150 of your textbook.
The pH scale goes from 0-14. It is logarithmic, which means that for each number difference on the scale there is a 10-fold change in the amount of hydrogen ions.
Carbon Dioxide and the pH of Water: When carbon dioxide (a gas) dissolves in water it can change the pH of water. You will complete a simple experiment showing this principle.
Obtain a 250 mL Flask filled with a solution of 150mL distilled water + 15 mL Bromothymol blue 0.2%. Bromothymol blue is an acid-base indicator. It is blue in neutral solutions and turn to green and yellow as the pH changes. Test the ph of the solution using a strip of pH paper and record below:
Color pH of Solution
Insert a straw into the solution allowing the end to touch the bottom of the beaker. Take a deep breath and blow slowly into the solution in a controlled manner. Caution: do not suck solution into your mouth. Repeat the process until a total of 5 breaths have been blown (you may have a team of people to help you). Test the ph of the solution using a strip of pH paper and record below:
Color pH of Solution
The acid that you have made by bubbling CO 2 into the water is called carbonic acid. This is the same process that is occurring as CO 2 dissolves in ocean water from the atmosphere. Fill in the following chemical equation by counting the total of each element on the left side and filling them into the right side: (Remember that if the chemical symbol does not have a subscript number after it,that means there is only
H2O (water) + CO2 (carbon dioxide) H_CO_ (carbonic acid)
The chemical formula for carbonic acid is: _________________________________
Referring to Table 16.1 on page 478, record the levels of CO2:
Pre-Industrial (circa 1750)
Present Concentration
Current Rate of Increase (%/yr) CO 2
Acids can have a profound effect on marine animals and plants that have shells made of calcium carbonate. In this part, you will investigate that effect. Using a pH strip,
What pH solutions would you test? Why? Remember, you will test two solutions.
Develop your hypothesis for this test just focusing on the pH value.
Place each shell fragment in its own container and label with the species, the shell weight and pH solution being tested. Fill the container with enough pH solution to cover the shell. Watch it fizz. The fizzing is the reaction of the calcium carbonate with the acid; it releases carbon dioxide. Let the shell soak for approximately a day. Remove the shell and wash with soap and water to remove the pH solution. Record the changes below. Shell Weight Initial Weight
After 1 day
After 1 week
% Weight lose over one week Species
Species
Species
Analyze your data: Create an excel graph showing the weights over time for each shell species. Attach the graph to the back of your lab report. Utilize your graph to answer the conclusion questions below.
What is meant by “ Anthropogenic ocean acidification
What range of pH changes are they estimating?