TERM 1
Ex.1:Ubiquity of Microorganisms
DEFINITION 1
1. We used trypticase soy agar medi 2. 37=organisms with warm-
blooded cells 22-25=organisms that gr ow on glaciers 3. fungi
grows larger and fluffy, while basteria h as a soft and glossy
appearance and tend to be cream w hite or yellow colored. >you
cannot visualize viruses on an agar pla te because there must be
bacteria for them to inhit and even t here they form plaques.
TERM 2
Ex.2:Pure Culture and Aseptic Technique
DEFINITION 2
1. asceptic technique is when you k eep your culture free of
contamination from the environmen t or other organisms. >prevent
contamination/infection >easier to o bserve a certain organism 2.a
pure culture is a population of cells re sulting from the the growth
of a single cell. 3.Because by spreadin g the bacteria on the
surface of a plate the cells are able t o form individual colonies
separated from other individual coloni es. used to obtain a pure
culture. 5. No, there is no specific rule based on quadrant.
TERM 3
Ex.3: Introduction to the compound light
microscope
DEFINITION 3
2. the field of vision becomes more na rrow/decreases as
magnification is increased. 4.increase the amount of light when
magnification is high and when viewin g a stained slide. 5. Iris
diaphram, dimer switch, condenser 6 . A parfocal is a lense that
stays in focus when magnification/foc al length is changed. 10.
resolution is the ability to distinguish tw o close objects as distinct
from one another rather than as one hazy object. Resolving power
is the minimum distance existing b/t 2 objects when those objects
TERM 4
Ex. 5: simple stains-positive and negative
stains
DEFINITION 4
1. morphology is the from,structure a nd configuration of an
organism, while arrangement is how the cells are arranged.
2.simple stains is a proceedure for sta ining bacteria consisting of a
single stain while differential stain stain s specific morphological
structures; usually a multuple stain. 3 . a negative stain creates a
clear organism on a black backgroun d. 4. there are 1000
nanometers in a micrometer and 10 00 micrometers in a
millimeter.
TERM 5
Ex. 6: Differential and Special Stains
DEFINITION 5
2. You heat fix a slide to cause the bacteria to adhere to the
glass slide. 4. cells are too old or over-discoloration. 5.
crystal violet:primary stain (both purple) >iodine
solution:mordant (both purple) >alcohol solution: discolorant
(gp:puple, gn:discolored) >sufranin solution: counter stain
(gp:purple, gn:pink)
