Partial preview of the text
Download Lab exam 1 | BIOL 208 - LAB GEN MICROBIOLOGY and more Quizzes Biology in PDF only on Docsity!
Iris Diaphragm
varies the amout of light
TERM 2
Condenser
DEFINITION 2
condenses light onto 1 spot (inside the iris) focuses the light
TERM 3
Total magnification
DEFINITION 3
calculated by multiplying the magnification of ocular lens by
objective lens
TERM 4
oil immersion
DEFINITION 4
eliminates the air gap between the specimen and objective
lens
TERM 5
why is oil immersion used with the 100x
objective?
DEFINITION 5
to capture the light
what is the advantage of parfocal
lenses
after viewing a specimen, there is little or no refocusing
TERM 7
would a shorter wavelength increase or
decrease a resolution?
DEFINITION 7
decrease
TERM 8
Media can be solid or liquid?
DEFINITION 8
true
TERM 9
Broth
DEFINITION 9
liquid growth media. (lacks structure)
TERM 10
Solid Medium
DEFINITION 10
contains Agar- solidifying agent. (has physical structure)
what color is bacillus subtilis?
bone color and smooth
TERM 17
motility
DEFINITION 17
capable of demonstrating movement by independent means
TERM 18
t/f: true independent motility depend on their
posession of flagella?
DEFINITION 18
true
TERM 19
Brownian Movement
DEFINITION 19
vibratorry motion of organisms and other particles
suspended in a fluid
TERM 20
Monotrichous
DEFINITION 20
single flagellum, located at the end of a cell
Amphitrichous
single flagellum at each pole
TERM 22
lophotrichous
DEFINITION 22
have a bundle of flagella at one end or both ends of a cell
TERM 23
Petrichous
DEFINITION 23
flagella are distributed uniformely over the surface of each
bacterial cell. (highly motile bacteria)
TERM 24
Candida albicans
DEFINITION 24
Yeast
TERM 25
Gardnerella vaginalis
DEFINITION 25
bacterial
what do we use to stop bacterial
motion?
use dye
TERM 32
aniline dyes
DEFINITION 32
theb est dyes to use- synthetic organic compounds made of
coal tar products
TERM 33
Basic stain
DEFINITION 33
positive charge chromophore that is attracted to the negative
charges outer surface of the microbial cell
TERM 34
E. Coli has what shape?
DEFINITION 34
rod
TERM 35
what dye is most difficult to
use?
DEFINITION 35
methylene blue
what does heat fixing
do?
inactivates the bacterial enzymes and sticks the cells to the
glass slide
TERM 37
normal, indigenous flora
DEFINITION 37
bacteria that are residents of the collection site
TERM 38
transiet organisms
DEFINITION 38
bacteria that are not residents of the collection site
TERM 39
what stain is used to differentiate
bacteria?
DEFINITION 39
gram stain
TERM 40
Alcohol in gram staining does what?
DEFINITION 40
decolorizes
sterilizing
kills the endospores (most difficult to kill)
TERM 47
Flagella stain
DEFINITION 47
allows for direct observation of flagella
TERM 48
Endospore stain
DEFINITION 48
Bacillus and clostridium are bacterial genera that produce
endospores
TERM 49
Endospore localization
DEFINITION 49
central ( middle of cell)terminal (end of the cell)subterminal (
between the end and and the middle of cells)
TERM 50
the cortex of an endospore is made of?
DEFINITION 50
cross linked peptidoglycan
Malachite green
negative dye
TERM 52
What is the counter stain in an endospore
slide?
DEFINITION 52
safranin
TERM 53
Gram stain: E
coli
DEFINITION 53
rods, red (negative)
TERM 54
Gram stain: Micrococcus Luteus
DEFINITION 54
cocci, tetrads, Purple color (positive)
TERM 55
Endospore stain:Bacillus Subtilis
DEFINITION 55
rods are red, endospores and green (negative)
