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Material Type: Lab; Professor: Lester; Class: MICROBIOLOGY; Subject: Biological Sciences; University: Montgomery College; Term: Unknown 1989;
Typology: Lab Reports
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Experiment #: none
Title: Gradient Plate: Isolation of Streptomycin Resistant Mutant
Live Organisms: E. coli
Changes: Procedure (Work in pairs or if in groups, do two sets per group)
What is the purpose of the streptomycin? Does streptomycin induce (cause) mutations? What are some of the possible mechanisms a mutant might use to circumvent the streptomycin?
First agar deep Put pencil under one end. Wait 15 minutes for agar to solidify.
Second agar deep + 1.0 mL streptomycin
Wait 15 minutes then spread 2 drops of E. coli on surface.