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Isolation of Coliform Bacteria From Mishandled Meat, Lab Reports of Microbiology

This PDF contains the answers to the lab which I got a 100% on.

Typology: Lab Reports

2022/2023

Available from 08/10/2023

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Name __________________________________________ Section _____________
Isolation of Coliform Bacteria from Mishandled Meat - Results & Interpretation 25 pts total
EMB agar plate results and interpretation:
Use the results for the EMB plate in “Isolation of Coliforms from Mishandled Meat - RESULTS” to
complete Table 1 and the associated questions.
Record and interpret the growth results for each area (#1, #2 and #3) on the EMB plate results.
Then, list the presumptive ID for EMB this would not be a specific genera or species, it would be
characteristics such as Gram positive or negative, non-coliform, ……. etc. (refer to the “Selective and
Differential” lab handouts, or your notebook.
Table 1. EMB plate results (12 pts)
EMB plate
Results AND Interpretation
Presumptive ID
Area
#1:
Record and interpret the
growth results for Area #1.
(2.5 pts)
**You must include ALL the
information that can be
determined to get full
credit.
Gram stain results for
Area #1 -
(Gram reaction, morphology
and arrangement) 1.5 pts
Alexa Campanella
SU23
Good growth - the organism is
not inhibited by eosin or
methylene blue
Growth is pink - the organism
ferments lactose and/or sucrose
with little acid production
Gram negative,
possible coliform
pf3
pf4
pf5

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Name __________________________________________ Section _____________

Isolation of Coliform Bacteria from Mishandled Meat - Results & Interpretation – 2 5 pts total

EMB agar plate results and interpretation:

Use the results for the EMB plate in “Isolation of Coliforms from Mishandled Meat - RESULTS” to complete Table 1 and the associated questions.

Record and interpret the growth results for each area (#1, #2 and #3) on the EMB plate results. Then, list the presumptive ID for EMB this would not be a specific genera or species, it would be characteristics such as Gram positive or negative, non-coliform, ……. etc. (refer to the “Selective and Differential” lab handouts, or your notebook.

Table 1. EMB plate results (12 pts)

EMB plate Results AND Interpretation Presumptive ID

Area

Record and interpret the growth results for Area #1. (2.5 pts) ****You must include ALL the information that can be determined to get full credit.**

Gram stain results for Area #1 - (Gram reaction, morphology and arrangement) – 1.5 pts

Table 1. (continued) - EMB plate results

EMB plate Results AND Interpretation Presumptive ID

Area

Record and interpret the growth results for Area # 2. (2.5 pts) ****You must include ALL the information that can be determined to get full credit.**

Gram stain results for Area # 2 - (Gram reaction, morphology, and arrangement) (1.5 pts)

Area

Record and interpret the growth results for Area # 3. (2.5 pts) ****You must include ALL the information that can be determined to get full credit.**

(Results AND Interpretation) (Presumptive ID)

Gram stain results for Area # 3 (Gram reaction, morphology and arrangement) (1.5 pts)

Salmonella-Shigella (SS) agar plate results and interpretation:

Use the results for the SS agar plate in “Isolation of Coliforms from Mishandled Meat - RESULTS” to complete Table 2 and the associated questions.

Record and interpret the growth results for each area (#4 and #5) on the SS agar plate results. Then, list the presumptive ID for SS agar this would be a specific genera or species (refer to the information about SS agar on page 3 in the “Isolation of Coliforms from Mishandled Meat - Background and Procedure”).

Table 2. Salmonella-Shigella (SS) agar plate results (7 pts)

SS agar plate Results AND Interpretation Presumptive ID

Area

Record and interpret the growth results for Area # 4. (2 pts) ****You must include ALL the information that can be determined to get full credit.**

Gram stain results for Area # 4 - (Gram reaction morphology and arrangement) – 1. 5 pts

Area

Record and interpret the growth results for Area # 5. (2 pts) ****You must include ALL the information that can be determined to get full credit.**

(Results AND Interpretation) (Presumptive ID)

Gram stain results for Area # 5 (Gram reaction, morphology and arrangement) (1.5 pts)

Use the information you recorded in Table 2 (SS results) to answer the following questions. ( 3 pts)

  1. Did any areas (# 4 or # 5 ) on the SS agar plate indicate coliforms were present in the ground beef? Explain your answer (1 pt)
  2. Were the Gram stain results for Areas # 4 and # 5 what you would expect based on the growth results for each area on the SS agar plate? Explain your answer (1 pt)
  3. What do the SS agar plate results tell you about how the meat was handled? (1 pt for a good answer or receive 1 extra point (2 pts) for a really good answer)