Download Isolation of Coliform Bacteria From Mishandled Meat and more Lab Reports Microbiology in PDF only on Docsity!
Name __________________________________________ Section _____________
Isolation of Coliform Bacteria from Mishandled Meat - Results & Interpretation – 2 5 pts total
EMB agar plate results and interpretation:
Use the results for the EMB plate in “Isolation of Coliforms from Mishandled Meat - RESULTS” to complete Table 1 and the associated questions.
Record and interpret the growth results for each area (#1, #2 and #3) on the EMB plate results. Then, list the presumptive ID – for EMB this would not be a specific genera or species, it would be characteristics such as Gram positive or negative, non-coliform, ……. etc. (refer to the “Selective and Differential” lab handouts, or your notebook.
Table 1. EMB plate results (12 pts)
EMB plate Results AND Interpretation Presumptive ID
Area
Record and interpret the growth results for Area #1. (2.5 pts) ****You must include ALL the information that can be determined to get full credit.**
Gram stain results for Area #1 - (Gram reaction, morphology and arrangement) – 1.5 pts
Table 1. (continued) - EMB plate results
EMB plate Results AND Interpretation Presumptive ID
Area
Record and interpret the growth results for Area # 2. (2.5 pts) ****You must include ALL the information that can be determined to get full credit.**
Gram stain results for Area # 2 - (Gram reaction, morphology, and arrangement) (1.5 pts)
Area
Record and interpret the growth results for Area # 3. (2.5 pts) ****You must include ALL the information that can be determined to get full credit.**
(Results AND Interpretation) (Presumptive ID)
Gram stain results for Area # 3 (Gram reaction, morphology and arrangement) (1.5 pts)
Salmonella-Shigella (SS) agar plate results and interpretation:
Use the results for the SS agar plate in “Isolation of Coliforms from Mishandled Meat - RESULTS” to complete Table 2 and the associated questions.
Record and interpret the growth results for each area (#4 and #5) on the SS agar plate results. Then, list the presumptive ID – for SS agar this would be a specific genera or species (refer to the information about SS agar on page 3 in the “Isolation of Coliforms from Mishandled Meat - Background and Procedure”).
Table 2. Salmonella-Shigella (SS) agar plate results (7 pts)
SS agar plate Results AND Interpretation Presumptive ID
Area
Record and interpret the growth results for Area # 4. (2 pts) ****You must include ALL the information that can be determined to get full credit.**
Gram stain results for Area # 4 - (Gram reaction morphology and arrangement) – 1. 5 pts
Area
Record and interpret the growth results for Area # 5. (2 pts) ****You must include ALL the information that can be determined to get full credit.**
(Results AND Interpretation) (Presumptive ID)
Gram stain results for Area # 5 (Gram reaction, morphology and arrangement) (1.5 pts)
Use the information you recorded in Table 2 (SS results) to answer the following questions. ( 3 pts)
- Did any areas (# 4 or # 5 ) on the SS agar plate indicate coliforms were present in the ground beef? Explain your answer (1 pt)
- Were the Gram stain results for Areas # 4 and # 5 what you would expect based on the growth results for each area on the SS agar plate? Explain your answer (1 pt)
- What do the SS agar plate results tell you about how the meat was handled? (1 pt for a good answer or receive 1 extra point (2 pts) for a really good answer)
