Download Introduction Biochemistry - Exam 2 - Fall 2001 | 694 301 and more Exams Biochemistry in PDF only on Docsity!
Biochemistry 694:301 Name___________________________ Second Test Dr. Deis Last 5 digits of SSN _______________ Thu. Oct. 25, 2001 Row Letter ____ Seat Number ______
This exam consists of two parts. Part I is multiple choice. Each of these 25 questions is worth two points. Answer the Part I questions on this sheet, below. Answer the Part II questions on the question pages.
Please use BLOCK CAPITAL letters like this --- A, B, C, D, E. Not lowercase!
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GRADE:
Part I Total _______
Part II:
II-1 _______ II-2 _______ II-3 _______ II-4 _______ II-5 _______
Part II Total _______
Total, I & II _______
- The difference between a Cerebroside and a Ganglioside is that A. one is a sphingolipid, the other a phosphatidyl lipid B. one contains a fatty acid amide, the other a fatty ester C. one contains a sugar head group, the other an oligosaccharide D. one has sugar phosphate, the other simply a sugar
- Eucaryotic cell membranes are strengthened by A. the cell wall D. polyunsaturated fats B. glycolipids E. all of the above C. proteins like Spectrin
- Cardiolipin is found in A. the mitochondrial inner membrane D. the lysosome B. the nuclear membrane E. the endoplasmic reticulum C. the eucaryotic cell membrane
- Primary Active Transport – fueled by (what) to transport (what)? A. sugars, sugars D. GDP, proteins B. ATP, ions E. none of the above C. cAMP, amino acids
- The Acetylcholine Receptor is composed of how many subunits? A. 1 D. 6 B. 4 E. 8 C. 5
- Potassium can not pass through the voltage gated Sodium Channel because A. inactivation prevents it D. K+^ is too large B. too much Sodium gets in way E. none of the above C. K+^ is too positive
- Protein Kinase C transfers a phosphate to Serine in substrate proteins containing the sequence RKGSLRQ. Like Protein Kinase A, PKC has a regulatory domain with a pseudosubstrate sequence. Which of the following would be a good candidate for this sequence? A. RAGSLRQ D. RKGSARQ B RKASLRQ E. RKGSLAQ C. RKGALRQ
- An inhibitor of the enzyme Acetylcholinesterase could be used as A. a sleeping pill D. antibiotic B. inhibitor of gastric acidity E. nerve gas C. anti-inflammatory
- You drink a glass of milk. An hour later you have intestinal cramps and other gut symptoms. Chances are you have A. galactosemia C. lactose intolerance B. diabetes D. malaria
- Glucose which has been oxidized to have a carboxyl group at C-6 is called: A. Gluconate D. Fructose B. Glucuronate E. Sedoheptulose C. Glucarate
- D-Xylulose forms a glycoside when a substituent is added to which position? A. 1 D. 4 B. 2 E. 5 C. 3
20.* Interpret the "chair" structure shown below. What is the name of the sugar represented? A. alpha-D-Mannose D. beta-D-Allose B. alpha-D-Glucose E. beta-D-Ribose C. alpha-D-Galactose
- When insulin signals ingestion of food, GLUT-4 receptors are A. rapidly synthesized D. translocated to outer membrane B. rapidly destroyed E. blocked by binding insulin C. put into inactive mode
- Aldolase forms a covalent connection between substrate and a residue of A. lysine D. glutamate B. serine E. alanine C. cysteine
- The normal mechanism of G3PDH is directly disrupted by A. cyanide D. citrate B. 2, 4 DNP E. arsenate C. Fru-2,6 BP
24.* The graph below represents the kinetics of PFK-I when F6P is held constant and ATP is varied. The lines marked "+" show the kinetics in the presence of what? A. cyclic AMP D. Glucose 6 P B. Calcium ion E. Fru-2,6-BP C. 2,3 BPG
- How many points is this question worth? A. 0 C. 2 B. 1 D. I don't know
PART II Answer these questions here on the question pages.
- a. Draw Phosphatidyl Serine, and show how it would be cleaved by Phospholipase C.
(3)
b. Draw a sketch which illustrates the Fluid Mosaic Model of membrane structure.
(2)
c. Where is Acetylcholinesterase located? Why is it such a fast acting enzyme? Be as specific as you can.
(3)
d. Briefly describe the process for an Excised Patch Mode Patch-Clamp Conductance experiment. What would one be trying to observe by doing this?
(2)
- a. Sketch the structure of IgG – show the chains and label the constant and variable regions within each chain.
(3)
b. What genes are involved in generation of antibody diversity? Diagram the germ-line DNA as done in class and in the text. Label the gene segments and tell what the abbreviations stand for.
(3)
c. Distinguish functionally between Class I and Class II MCH proteins. For a complete answer you have to discuss various T-cells, etc.
(4)
4.* a. What cofactors are represented below?
(4)
b. Draw the disaccharide D-mannopyranosyl α1-4 β D-xylulofuranose in the Haworth projection. For partial credit just draw D-Mannose and D-Xylulose in the Fischer projection.
(4)
c. Draw the alpha and beta anomers of D-Fructopyranose (2)
