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intro to biotechnology - exam 1 practice questions, Exams of Biotechnology

intro to biotechnology - exam 1 practice questions

Typology: Exams

2023/2024

Available from 02/09/2024

EmmaMoss
EmmaMoss 🇬🇧

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intro to biotechnology - exam 1 practice
questions
1. Bacteria contain extrachromosomal molecules of DNA called plasmids which are
circular and used for recombinant DNA technology
true
2. A restriction enzyme cuts only double-helical segments that contain a particular
sequence, and it makes its incisions only within that sequence--known as a
"recognition sequence"
true
3. Kary Mullis won the Nobel Prize for development of this novel technique of PCR
in
1993
4. Restriction enzymes...
A. were discovered during study of bacterial resistance to some
phage infections.
B. may leave "sticky" or "blunt" ends in DNA.
C. are used to map DNA.
D. recognize palindromic sequences.
E. all of the above
F. all of the above
5. The number of primers required for conventional Polymerase Chain Reaction
(PCR) is/are
2
6. In recombinant methods, the term "vector" refers to
plasmid or other agent used to transfer DNA into a living cell.
7. Which of the following would be considered a transgenic organism
a rat with rabbit hemoglobin genes
8. A PCR reaction mix has the following....
A. DNTP
B. Forward and reverse Primers
C. DNA polymerase
D. Magnesium chloride
E. All the above
e. all of the above
9. _____ involves applying a brief (milliseconds) pulse high voltage electricity to
create tiny holes in the bacterial cell wall that allows DNA to enter.
electroporation
pf3
pf4
pf5

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questions

  1. Bacteria contain extrachromosomal molecules of DNA called plasmids which are circular and used for recombinant DNA technology true
  2. A restriction enzyme cuts only double-helical segments that contain a particular sequence, and it makes its incisions only within that sequence--known as a "recognition sequence" true
  3. Kary Mullis won the Nobel Prize for development of this novel technique of PCR in 1993
  4. Restriction enzymes... A. were discovered during study of bacterial resistance to some phage infections. B. may leave "sticky" or "blunt" ends in DNA. C. are used to map DNA. D. recognize palindromic sequences. E. all of the above F. all of the above
  5. The number of primers required for conventional Polymerase Chain Reaction (PCR) is/are 2
  6. In recombinant methods, the term "vector" refers to plasmid or other agent used to transfer DNA into a living cell.
  7. Which of the following would be considered a transgenic organism a rat with rabbit hemoglobin genes
  8. A PCR reaction mix has the following.... A. DNTP B. Forward and reverse Primers C. DNA polymerase D. Magnesium chloride E. All the above e. all of the above
  9. _____ involves applying a brief (milliseconds) pulse high voltage electricity to create tiny holes in the bacterial cell wall that allows DNA to enter. electroporation

questions

  1. Imagine that, sometime in the future, human space explorers discover life on another planet, and that this life has DNA as its genetic material. Which of the following could be used to study the genetics of this extraterrestrial life? A. Cloning B. Gel electrophoresis C. Polymerase chain reactions D. Restriction enzymes E. All of the above e. all of the above
  2. Which of the following is a native protein isolated from bacteria and used to digest starch? amylase
  3. The major application of Trichoderma sps. is the use of the enzyme ____ in pulp bleaching for paper. xylanase
  4. Baker's yeast or ________ are ethanol-fermenting yeasts commonly used when making bread and pizza dough. Saccharomyces cerevisiae
  5. The use of a brief electrical shock to introduce DNA into bacterial cells is called electroporation
  6. Plant Biotechnology chiefly involves the introduction of______ genes into economically important plant species foreign
  7. which area of biotechnology primarily involves using living organisms to process, degrade and clean up environmental pollution? bioremediation
  8. the use of computer hardware and software to analyze biological data, such as DNA sequence comparison data, is known as ___ bioinformatics
  9. combining DNA from different sources is an example of recombinant DNA technology

questions

  1. antiboyd-producing cells in humans are called ___ plasma cells
  2. the vaccine for which of these pathogens is an inactivated vaccine? influenza
  3. which type of vaccine involves using a weakened pathogen incapable of replicating? attenuated vaccines
  4. the antibiotic bacitarium is isolated from ___. B. subtilis
  5. the antibiotic penicillin is isolated from __. P. notatum
  6. during calcium choloride transformation of bacteria, during which step does plasmid DNA enter the bacterial cells? brief incubation at 42 C
  7. which by-product of alcohol fermentation causes bread dough to rise and creates bubbles in champagne? carbon dioxide
  8. yeast can grow ___. under both aerobic and anaerobic conditions
  9. You just cloned a new gene from mice. Which of the following techniques would be the best choice to use if you wanted to determine the number of copies of this gene in the mouse genome southern blot analysis (DNA)
  10. a ____ is a single-stranded DNA molecule attached to a radioactive or fluorescent compound that is complementary to a specific sequence of DNA. such pieces if DNA are used to identify and study cloned gene in hybridization experiments. probe
  11. which of the following is an incorrect statement about restriction enzymes restriction enzymes create phosphodiester bonds between pieces of DNA in a cloning experiment

questions

  1. based on what you know about which organisms naturally produce restriction enzymes & how restriction enzymes are named. which of the following enzymes is isolated from the bacterium bacillus amyloliquefaciens? BamHI
  2. which of the following techniques is most commonly used to separate & analyze DNA by size? Agarose gel electrophoresis
  3. during molecular cloning, a gene of interest insulin is inserted into bacterial structure called ____ and enters bacterial cells to a process called ____ plasmid; transformation