intro to biotechnology - exam 1 practice
questions
1. Bacteria contain extrachromosomal molecules of DNA called plasmids which are
circular and used for recombinant DNA technology
true
2. A restriction enzyme cuts only double-helical segments that contain a particular
sequence, and it makes its incisions only within that sequence--known as a
"recognition sequence"
true
3. Kary Mullis won the Nobel Prize for development of this novel technique of PCR
in
1993
4. Restriction enzymes...
A. were discovered during study of bacterial resistance to some
phage infections.
B. may leave "sticky" or "blunt" ends in DNA.
C. are used to map DNA.
D. recognize palindromic sequences.
E. all of the above
F. all of the above
5. The number of primers required for conventional Polymerase Chain Reaction
(PCR) is/are
2
6. In recombinant methods, the term "vector" refers to
plasmid or other agent used to transfer DNA into a living cell.
7. Which of the following would be considered a transgenic organism
a rat with rabbit hemoglobin genes
8. A PCR reaction mix has the following....
A. DNTP
B. Forward and reverse Primers
C. DNA polymerase
D. Magnesium chloride
E. All the above
e. all of the above
9. _____ involves applying a brief (milliseconds) pulse high voltage electricity to
create tiny holes in the bacterial cell wall that allows DNA to enter.
electroporation
