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A laboratory exploration for students to identify nutrients in various food samples using different tests. The major types of nutrients - carbohydrates, proteins, and lipids - and their functions in the body. Students will learn how to test for these nutrients using the benedict, lugol, biuret, and sudan red tests. The document also includes activities and discussions to help students understand the importance of knowing the nutritional content of food.
Typology: Summaries
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Name: Date:
Vocabulary: carbohydrate, disaccharide, lipid, monosaccharide, polysaccharide, protein, starch
Gizmo Warm-up
Have you ever wondered what is in your food? Scientists
use a variety of tests to determine the nutritional content of
food. You will learn four of those tests with the Identifying
Nutrients Gizmo.
Food sample holder. Below the Benedict test , click
the Test button. What is done in the Benedict test?
(fruit sugar). In contact with monosaccharides, the Benedict solution turns from blue to
green to orange.
Does Sample A contain monosaccharides?
Note: Disaccharides such as sucrose (table sugar) and lactose (milk sugar) are more
complex than monosaccharides. The Benedict test does not detect disaccharides directly.
There are six major
nutrients: Carbohydrates (CHO), Lipids (fats), Proteins, Vitamins, Minerals, Water.
The body uses three main nutrients to function carbohydrate, protein, and fat. These nutrients are digested
into simpler compounds. Carbohydrates are used for energy (glucose). Fats are used for energy
after they are broken into fatty acids.
Solutions were added and it was heated in a water bath for f min
yes
Activity B:
Nutrients and
food types
Get the Gizmo ready:
Question: What nutrients does each food sample contain?
M. (Sample A has been done for you as an example.) Record results on the table below.
Carbohydrate
Tests
Protein
Test
Lipids
Test
Test results – are these nutrients
present?
Food
sample
Benedict
test
Lugol
test
Biuret
test
Sudan
Red
test
Mono
-
saccharides
Starches
Proteins
Lipids
A + – – – Yes No No No
A. Is sample E most likely to be steak, bread, or butter? Justify your answer.
B. Is sample G most likely to be table sugar, pasta, or olive oil? Justify your answer.
C. Is sample M most likely to be chicken, rice, a mango, or butter? Justify your answer.
(Activity B continued on next page)
no
yes yes
Steak because it has proteins and fats
no no no
no
no yes
no no
Sample G would be table sugar because we did not test for disaccharides and they don't show the test
results for proteins, carbohydrates or lipids.
Rice because it is a starch and food sample M tested positive for the Lugol test.
Every nutrient plays a role and is very important to our body. We have to know about nutrients so
that we can apply each one to out bodies in the correct form.
Activity B (continued from previous page)
Carbohydrate
Tests
Protein
Test
Lipids
Test
Test results – are these nutrients
present?
Food
sample
Benedict
test
Lugol
test
Biuret
test
Sudan
Red
test
Mono
-
saccharides
Starches
Proteins
Lipids
teacher. Try to come up with a type of food that corresponds to each sample.
carbohydrates, proteins, and lipids. Too much sugar (monosaccharides and other simple
sugars) is unhealthy. Fruits and vegetables are important sources of vitamins and minerals.
Do you consider your diet balanced and healthy? Why or why not?
no no yes
yes
no
yes no
no
no no yes yes
no no no no
no no yes yes
yes no no no
no no yes yes
yes no no no
no no yes yes
no yes no no
no no no no
yes no no no
no no yes yes
no yes no no
no no yes yes
I think my diet is balanced because it contains relatively even amounts of
carbohydrates, proteins, and lipids and I don't really eat that much sugar.