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Name
BIO 323 – Microbiology lab
Lab Section
Unknown Identification – 55 points total
Use the Table of possible unknowns on page 3 of to assist you in answering questions 2 - 6.
1. Which two characteristics are the most important to identify when starting to identify an unknown bacteria? (2 pts)
2. Which of the eight bacteria listed in the Table on page 3 have a rod shaped morphology?
You must list all that apply or you will not get full credit. (1 pt)
3. Which of the eight bacteria listed in the Table on page 3 have a cocci shaped morphology?
You must list all that apply or you will not get full credit. (1.5 pts)
4. Which of the eight bacteria listed in the Table on page 3 have a spirilli shaped morphology?
(0.5 pts)
5. Which of the eight bacteria listed in the Table on page 3 are Gram positive? You must list all that apply or you will not get full credit. (2.5 pts)
7. Which of the eight bacteria listed in the Table on page 3 have the ability to produce an endospore
if conditions become to harsh for its survival?
You must list all that apply or you will not get full credit. ( 2 pts)
You will need to reference other OUTSIDE material to answer questions 7 - 9.
The information is NOT included in the Table on page 3.
8. Which of the eight bacteria listed in the Table on page 3 possess a capsule? ( 1 pt)
Hint: S. aureus, S. epidermidis, and E.coli DO NOT have a capsule even though some online sites mat say they do.
All the information online is not necessarily correct! Refer to your textbook or reputable scientific sites online.
9. Which of the eight bacteria listed in the Table on page 3 possess flagella?
You must list all that apply or you will not get full credit. ( 3 pts)
6. Which of the eight bacteria listed in the Table on page 3 are Gram negative?
You must list all that apply or you will not get full credit. (1.5 pts)
Use the results for Unknown # 2 included in the file labeled "RESULTS for the Identification of an Unknown Bacteria
assignment"to complete the last row in the table below and identify Unknown # 2.
Identification of an Unknown Bacteria # 2 - Results - 10 pts
Organism
Gram stain Morphology and Result of Gram stain
Mannitol Salt Agar (MSA)
MacConkey Agar (MAC)
Eosin Methylene Blue Agar (EMB) Starch Gelatin Glucose Mannitol^ Lactose
Hydrogen sulfide
Urease Citrate
Number of Results That Match Morphology (^) ResultGram Growth Fermentation(see notes) Growth Fermentation(see notes) Growth Fermentation(see notes)
Proteus vulgaris Short rod - - - +
growth is normal color
growth is normal color
Escherichia coli Short rod - - - +
growth is pink
growth is metallic green/black
Klebsiella pneumoniae Short rod - - - +
growth is normal color growth is pink
Micrococcus luteus (^) Cocci + - - - - - - - + - - - - + +
Bacillus cereus Rod + - - - - - - + - + - - - - +
Bacillus subtilis Rod + +
media turned yellow
Staphylococcus epidermidis Cocci + + - - - - - - - + - + - - -
Staphylococcus aureus Cocci + +
media turned yellow
Results for
Unknown # 2
(1 pt) (1 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt)
Organism
Gram stain Morphology and Result of Gram stain
Mannitol Salt Agar (MSA)
MacConkey Agar (MAC)
Eosin Methylene Blue Agar (EMB) Starch Gelatin Glucose Mannitol^ Lactose
Hydrogen sulfide
Urease Citrate
Number of Results That Match Morphology (^) ResultGram Growth Fermentation(see notes) Growth Fermentation(see notes) Growth Fermentation(see notes)
Proteus vulgaris Short rod - - - +
growth is normal color
growth is normal color
Escherichia coli Short rod - - - +
growth is pink
growth is metallic green/black
Klebsiella pneumoniae Short rod - - - +
growth is normal color growth is pink
Micrococcus luteus (^) Cocci + - - - - - - - + - - - - + +
Bacillus cereus Rod + - - - - - - + - + - - - - +
Bacillus subtilis Rod + +
media turned yellow
Staphylococcus epidermidis Cocci + + - - - - - - - + - + - - -
Staphylococcus aureus Cocci + +
media turned yellow
Results for
Unknown # 3
(1 pt) (1 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt)
Use the results for Unknown # 3 included in the file labeled "RESULTS for the Identification of an Unknown Bacteria
assignment"to complete the last row in the table below and identify Unknown # 3.
Identification of an Unknown Bacteria # 3 - Results - 10 pts
