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Identification of an Unknown Bacteria Lab, Lab Reports of Microbiology

This PDF contains the answers to the lab which I got a 100% on.

Typology: Lab Reports

2022/2023

Available from 08/10/2023

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Name
BIO 323 – Microbiology lab
Lab Section
Unknown Identification 55 points total
Use the Table of possible unknowns on page 3 of to assist you in answering questions 2 - 6.
1. Which two characteristics are the most important to identify when starting to identify an
unknown bacteria? (2 pts)
2.Which of the eight bacteria listed in the Table on page 3 have a rod shaped morphology?
You must list all that apply or you will not get full credit. (1 pt)
3.Which of the eight bacteria listed in the Table on page 3 have a cocci shaped morphology?
You must list all that apply or you will not get full credit. (1.5 pts)
4.Which of the eight bacteria listed in the Table on page 3 have a spirilli shaped morphology?
(0.5 pts)
5.Which of the eight bacteria listed in the Table on page 3 are Gram positive?
You must list all that apply or you will not get full credit. (2.5 pts)
Alexa Campanella
SU23
The morphology and morphological arrangement and if the bacteria is Gram positive or
negative.
Proteus vulgaris, Escherichia coli, Klebsiella pneumoniae, Bacillus cereus, and Bacillus
subtilis.
Micrococcus luteus, Staphylococcus epidermidis, and Staphylococcus aureus.
None.
Micrococcus luteus, Bacillus cereus, Bacillus subtilis, Staphylococcus epidermidis, and
Staphylococcus aureus.
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Name

BIO 323 – Microbiology lab

Lab Section

Unknown Identification – 55 points total

Use the Table of possible unknowns on page 3 of to assist you in answering questions 2 - 6.

1. Which two characteristics are the most important to identify when starting to identify an unknown bacteria? (2 pts)

2. Which of the eight bacteria listed in the Table on page 3 have a rod shaped morphology?

You must list all that apply or you will not get full credit. (1 pt)

3. Which of the eight bacteria listed in the Table on page 3 have a cocci shaped morphology?

You must list all that apply or you will not get full credit. (1.5 pts)

4. Which of the eight bacteria listed in the Table on page 3 have a spirilli shaped morphology?

(0.5 pts)

5. Which of the eight bacteria listed in the Table on page 3 are Gram positive? You must list all that apply or you will not get full credit. (2.5 pts)

7. Which of the eight bacteria listed in the Table on page 3 have the ability to produce an endospore

if conditions become to harsh for its survival?

You must list all that apply or you will not get full credit. ( 2 pts)

You will need to reference other OUTSIDE material to answer questions 7 - 9.

The information is NOT included in the Table on page 3.

8. Which of the eight bacteria listed in the Table on page 3 possess a capsule? ( 1 pt)

Hint: S. aureus, S. epidermidis, and E.coli DO NOT have a capsule even though some online sites mat say they do.

All the information online is not necessarily correct! Refer to your textbook or reputable scientific sites online.

9. Which of the eight bacteria listed in the Table on page 3 possess flagella?

You must list all that apply or you will not get full credit. ( 3 pts)

6. Which of the eight bacteria listed in the Table on page 3 are Gram negative?

You must list all that apply or you will not get full credit. (1.5 pts)

Use the results for Unknown # 2 included in the file labeled "RESULTS for the Identification of an Unknown Bacteria

assignment"to complete the last row in the table below and identify Unknown # 2.

Identification of an Unknown Bacteria # 2 - Results - 10 pts

Organism

Gram stain Morphology and Result of Gram stain

Mannitol Salt Agar (MSA)

MacConkey Agar (MAC)

Eosin Methylene Blue Agar (EMB) Starch Gelatin Glucose Mannitol^ Lactose

Hydrogen sulfide

Urease Citrate

Number of Results That Match Morphology (^) ResultGram Growth Fermentation(see notes) Growth Fermentation(see notes) Growth Fermentation(see notes)

Proteus vulgaris Short rod - - - +

growth is normal color

growth is normal color

Escherichia coli Short rod - - - +

growth is pink

growth is metallic green/black

Klebsiella pneumoniae Short rod - - - +

growth is normal color growth is pink

Micrococcus luteus (^) Cocci + - - - - - - - + - - - - + +

Bacillus cereus Rod + - - - - - - + - + - - - - +

Bacillus subtilis Rod + +

media turned yellow

Staphylococcus epidermidis Cocci + + - - - - - - - + - + - - -

Staphylococcus aureus Cocci + +

media turned yellow

Results for

Unknown # 2

(1 pt) (1 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt)

Organism

Gram stain Morphology and Result of Gram stain

Mannitol Salt Agar (MSA)

MacConkey Agar (MAC)

Eosin Methylene Blue Agar (EMB) Starch Gelatin Glucose Mannitol^ Lactose

Hydrogen sulfide

Urease Citrate

Number of Results That Match Morphology (^) ResultGram Growth Fermentation(see notes) Growth Fermentation(see notes) Growth Fermentation(see notes)

Proteus vulgaris Short rod - - - +

growth is normal color

growth is normal color

Escherichia coli Short rod - - - +

growth is pink

growth is metallic green/black

Klebsiella pneumoniae Short rod - - - +

growth is normal color growth is pink

Micrococcus luteus (^) Cocci + - - - - - - - + - - - - + +

Bacillus cereus Rod + - - - - - - + - + - - - - +

Bacillus subtilis Rod + +

media turned yellow

Staphylococcus epidermidis Cocci + + - - - - - - - + - + - - -

Staphylococcus aureus Cocci + +

media turned yellow

Results for

Unknown # 3

(1 pt) (1 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt)

Use the results for Unknown # 3 included in the file labeled "RESULTS for the Identification of an Unknown Bacteria

assignment"to complete the last row in the table below and identify Unknown # 3.

Identification of an Unknown Bacteria # 3 - Results - 10 pts