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This PDF contains the answers to the lab which I got a 100% on.
Typology: Lab Reports
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Use the Table of possible unknowns on page 3 of to assist you in answering questions 2 - 6.
1. Which two characteristics are the most important to identify when starting to identify an unknown bacteria? (2 pts)
2. Which of the eight bacteria listed in the Table on page 3 have a rod shaped morphology?
You must list all that apply or you will not get full credit. (1 pt)
3. Which of the eight bacteria listed in the Table on page 3 have a cocci shaped morphology?
You must list all that apply or you will not get full credit. (1.5 pts)
4. Which of the eight bacteria listed in the Table on page 3 have a spirilli shaped morphology?
(0.5 pts)
5. Which of the eight bacteria listed in the Table on page 3 are Gram positive? You must list all that apply or you will not get full credit. (2.5 pts)
Organism
Gram stain Morphology and Result of Gram stain
Mannitol Salt Agar (MSA)
MacConkey Agar (MAC)
Eosin Methylene Blue Agar (EMB) Starch Gelatin Glucose Mannitol^ Lactose
Hydrogen sulfide
Urease Citrate
Number of Results That Match Morphology (^) ResultGram Growth Fermentation(see notes) Growth Fermentation(see notes) Growth Fermentation(see notes)
Proteus vulgaris Short rod - - - +
growth is normal color
growth is normal color
Escherichia coli Short rod - - - +
growth is pink
growth is metallic green/black
Klebsiella pneumoniae Short rod - - - +
growth is normal color growth is pink
Micrococcus luteus (^) Cocci + - - - - - - - + - - - - + +
Bacillus cereus Rod + - - - - - - + - + - - - - +
Bacillus subtilis Rod + +
media turned yellow
Staphylococcus epidermidis Cocci + + - - - - - - - + - + - - -
Staphylococcus aureus Cocci + +
media turned yellow
(1 pt) (1 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt)
Organism
Gram stain Morphology and Result of Gram stain
Mannitol Salt Agar (MSA)
MacConkey Agar (MAC)
Eosin Methylene Blue Agar (EMB) Starch Gelatin Glucose Mannitol^ Lactose
Hydrogen sulfide
Urease Citrate
Number of Results That Match Morphology (^) ResultGram Growth Fermentation(see notes) Growth Fermentation(see notes) Growth Fermentation(see notes)
Proteus vulgaris Short rod - - - +
growth is normal color
growth is normal color
Escherichia coli Short rod - - - +
growth is pink
growth is metallic green/black
Klebsiella pneumoniae Short rod - - - +
growth is normal color growth is pink
Micrococcus luteus (^) Cocci + - - - - - - - + - - - - + +
Bacillus cereus Rod + - - - - - - + - + - - - - +
Bacillus subtilis Rod + +
media turned yellow
Staphylococcus epidermidis Cocci + + - - - - - - - + - + - - -
Staphylococcus aureus Cocci + +
media turned yellow
(1 pt) (1 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt) (0.5 pt)