Download ICHS MICROBIOLOGY LAB 06 EXAM WITH COMPLETE SOLUTIONS!! and more Exams Advanced Education in PDF only on Docsity!
ICHS MICROBIOLOGY SOLUTI LAB 06 EONS!XAM WI! TH COMPLETE
Protection of the Central Nervous System - ANSWER 1 - Meninges 2 - Cerebrospinal Fluid3 - Brain 4 - Spinal Cord What type of meningitis is the most dangerous? Fungal meningitisAutoimmune meningitis Viral meningitis Bacterial meningitis - ANSWER Bacterial meningitis "Bacterial meningitis has high morality rates, and must be promptly treated withantibiotics."
How can the doctor rapidly determine if there are bacteria present in the cerebrospinalfluid (CSF)? - ANSWER Collect a few milliliters of CSF and send it for a Gram stain
What is the Gram Stain method? - ANSWER Staining method used to differentiatebacterial species into two groups
How long does it take to perform and analyze the Gram stain? 1 - 2 hours
48 hours 24 hours A few minutes - ANSWER A few minutes Hans: Do you know what two groups that i am talking about are named? Gram-visible and Gram-invisible Gram-positive and Gram-negativeGram-cocci and Gram-rods Gram-variable and Gram-constant - ANSWER Gram-positive and Gram-negative Hans: What is the outcome of a well-executed Gram stain? - ANSWER Gram- positiveappear purple, and Gram-negative appear pink
Hans: Why is the bacterial cell well of such great importance? Antibiotics can target the cell wallAll answers are correct The cell wall is involved in inducing disease in the infected hostThe cell wall is essential for bacterial viability - ANSWER All answers are correct
Hans: Where do we find the Teichoic acids? - ANSWER Gram-positive bacteria Hans: Where are the lipopolysaccharides found? - ANSWER The outer membrane ofGram-negative bacteria
What is the primary stain used, that colors all bacteria purple? - ANSWER Crystal violet
To exclude Gram-variable bacteria To determine if an unknown sample is Gram-positive or negativeTo verify that the Gram stain was performed properly To distinguish between Gram-positive and Gram-negative bacteria - ANSWER To verifythat the Gram stain was performed properly
Is the positive control properly stained? - ANSWER Yes. It appears Gram-positive, withbacteria stained purple
Is the negative control properly stained? - ANSWER Yes. it appears Gram-negative, withbacteria stained pink
Figure 1: CSF sample with Gram-negative bacteria. The big circular pink structures (A)are human cells, while the small pink structures are Gram-negative bacteria (B)
Which circle contains bacteria? A contains bacteriaThere are no bacteria present B contains bacteria - ANSWER B contains bacteria How would you describe the bacteria found in the CSF sample? They appear Gram-negativeThey appear Gram-variable (both positive and negative) They appear Gram-positive - ANSWER They appear Gram-negative What morphological group do the bacteria in the CSF sample belong to?
Cocci BacilliOthers - ANSWER Cocci
How are the Cocci in the CSF sample organized? StreptococciDiplococci Staphylococci - ANSWER Diplococci
