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BI 381 Fall 2005 Final Exam: Genetics and Molecular Biology - Prof. Allen C. Gathman, Exams of Molecular biology

Southeast Missouri State University (SEMO)Molecular biology
Prof. Allen C. Gathman

The instructions and questions for the final exam of the bi 381: genetics and molecular biology course from fall 2005. The exam covers topics such as genomic and cdna libraries, intron removal and exon splicing, terminator sequences, nucleotide excision repair, and human genetics.

Typology: Exams

Pre 2010

Uploaded on 08/08/2009

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BI 381 Fall 2005
Final Exam
Instructions:
1. Relax.
2. Write your name on the back of the last page only.
1. (5 points each)
a. Discuss the advantages and disadvantages of genomic libraries versus cDNA libraries.
b. When plating bacteria containing recombinant plasmids, it's common practice to put X-Gal
and ampicillin in the media. What does each of these do in this situation, and why would you
want it in the media?
c. In dideoxy sequencing, each reaction contains the four dNTPs and one ddNTP. For instance,
one reaction has ddATP, as well as dATP and the other three dNTPs. Why must this reaction
contain both ddATP and dATP? What would happen if either one were left out? Explain.
d. What does Pol I do in E. coli DNA replication? Explain.
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Download BI 381 Fall 2005 Final Exam: Genetics and Molecular Biology - Prof. Allen C. Gathman and more Exams Molecular biology in PDF only on Docsity!

BI 381 Fall 2005 Final Exam Instructions:

  1. Relax.
  2. Write your name on the back of the last page only.
  3. (5 points each) a. Discuss the advantages and disadvantages of genomic libraries versus cDNA libraries. b. When plating bacteria containing recombinant plasmids, it's common practice to put X-Gal and ampicillin in the media. What does each of these do in this situation, and why would you want it in the media? c. In dideoxy sequencing, each reaction contains the four dNTPs and one ddNTP. For instance, one reaction has ddATP, as well as dATP and the other three dNTPs. Why must this reaction contain both ddATP and dATP? What would happen if either one were left out? Explain. d. What does Pol I do in E. coli DNA replication? Explain.
  1. (10 points) Describe the process of intron removal and exon splicing in a eukaryotic RNA transcript. Be as specific as possible about the snurps involved and the sequence of events.
  2. (10 points)Cheng et al. concluded that the effectiveness of an intrinsic terminator depends on BOTH the stability of the stem in the RNA and the specific sequence of nucleotides within the stem. Referring to specific terminators in the figure on the last page of the test, provide arguments to support BOTH parts of this conclusion.
  1. A Klinefelter’s syndrome man (XXY) has normal color vision. His mother was colorblind and his father had normal color vision. (Remember that colorblindness is caused by the recessive allele c, and any individual with the dominant allele C will have normal color vision. The gene C/c is on the X chromosome). a. (10 points) How did his abnormal karyotype originate, when, in whom, in what kind of cell division? b. (10 points) Diagram the cell division referred to in part a, showing the chromosomes before and after the abnormal event occurs, with the pertinent alleles marked on them.
  1. ( 5 points each) a. Why are even-ploids fertile, and odd-ploids sterile? Explain. b. Based on the results of Guo and Birchler's study, why do trisomics have phenotypic abnormalities? Explain. c. Why do trisomy-X humans have less severe phenotypic effects than trisomy-13 humans? d. How did the human chromosome 2 form during the evolution of the primates?