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Technical details about biosera's foetal bovine serum (fbs), including product codes, collection and filtration processes, sterility and virus testing, endotoxin levels, osmolality, and haemoglobin measurement. The document also covers cell culture tests and the effects of heat inactivation.
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Typology: Lecture notes
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Product Codes FB-1051H FB-1350H FB-1365H FB-1345H FB-1360H FB-1370H FB-1000H FB-1001H FB-1280H FB-1200H
Collected from the source: When searchers choose their serum an important factor that should be taken into consideration is the source, which also emphasises the traceability of the serum. Our system of vertical integration allows us to be certain of the origins and traceability of our FBS. Each batch is rigorously controlled, from the collection of serum, and in all the stages of its treatment and production on our premises through to final packaging. Biosera Foetal Bovine Serum is derived from clotted whole blood aseptically collected from fetus via cardiac puncture.
Filtration: Final Filter Size: 0.1 μm x 3
Sterility: All sera are tested for the absence of aerobic and anaerobic bacteria, fungi, yeast and mycoplasma. The sterility test is based on the European Pharmacopoeia requirements. The sera are tested for the absence of mycoplasma by culture.
Virus tested: All of our serum is tested for:
Endotoxin: All sera are tested to determine the levels of endotoxins. Biosera FBS carry out a chromokinetic test (quantitative test) The endotoxin reagent is standardized against the US reference endotoxin. The method is based on “the bacterial endotoxins test”, US pharmacopoeia 23rd^ revision.
Osmolality: Determined by a lowered freezing temperature. The osmometer is calibrated against standard solution.
Haemoglobin: The haemoglobin level is measured by spectrophotometer.
Cell Culture Biological performance is assessed using cell culture medium supplemented with the serum being tested. During the test period, cultures are examined microscopically for any morphological abnormalities that may indicate toxic components in the serum.
Cell Culture Tests Cell Growth, Plating Efficiency, Cloning Efficiency
Cell Lines Tested The following cell lines are tested with the serum: HELA – Human cancer cell line from uterus L929 – Fibroblastic cells which came from adipose tissue of mouse SP2/0-AG14 – cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8, the growth mode is the suspension MRC-5 – diploidic cells (2n = 46 chromosomes), fibroblastic derived from human embryonic lung
Country of Origin The country in which the serum was taken from the donor/animal Biosera FBS is sourced from the following Countries
FB-1051H – Canada FB-1350H – USA FB-1365H – Chile FB-1345H – Central America FB-1360H – Mexico FB-1370H – Australia FB-1000H – Europe/South America FB-1001H – South America FB-1280H – France FB-1200H - France
Heat Inactivation Sera are heat inactivated to inactivate complement. The complement can lead to complement mediated cell lysis. Immunological studies justify the need to heat inactivate the serum. The treatment is a heating of the serum at 56°C during 30 minutes.
This treatment can modify the colour of the serum. This is normal and it does not compromise the quality of the product for the cell culture.
Note : To heat the serum during a long period can reduce or destroy the growth factors. It can also increase the build of precipitates that are frequently confounded with contamination.
Effects of the heat inactivation:
- Destruction of proteins - Precipitation of fibrin - Destruction of fibrinogen - Deterioration total or partial of vitamins - Decrease of the growth factors concentration - Destruction of the LDH - Decrease of the amylase concentration - Destruction of the alkaline phosphatase - Deterioration of the IgG, IgE, IgM