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Exam 2 with Solution - Fundamentals of Microbiology | MBIO 3813, Exams of Biology

Material Type: Exam; Professor: Nagle; Class: Fundamentals of Microbiology; Subject: MICROBIOLOGY; University: University of Oklahoma; Term: Fall 2009;

Typology: Exams

2010/2011

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Mbio 3813
Name
Lecture Exam 2
26 March 2009 Last 4 of ID No.
INSTRUCTIONS:
Write Name and Last four digits of ID No. on pp. 1 and 2 of exam.
SCANTRON:
Last Name, space, First Name
in NAME BOX
and Last 4 digits of ID
in
IDENTIFICATION NUMBER BOX.
Fill
in the circles before time runs out.
No
extra time will be given!
All
books and notes must be closed. No electronic devices of any kind may be
used
Cell Phone Alert:
Ifyour cell phone is heard ringing during the exam, there is a mandatory 5 (’five) point penalti’. Ifyou
expect a very important call, identify yourself to the instructor for special arrangements.
Academic Misconduct. Submission of the exam indicates tacit agreement with the statement "I have neither given nor
received inappropriate aid in completing this exam." Using crib sheets, electronic communication, looking at another
person’s paper, or any other activities which compromise the integrity of the exam are unacceptable and will be dealt with.
A. ESSAY QUESTION (20pIs):
Choose one topic from the following list. Write the Roman Numeral in the small box on p. 1.
Write your answer to the topic in the large box. No material outside the big box will be considered or graded Please write
legibly!
Criteria for essay: 15 points for accuracy and thoroughness of answer. A
list, table, or drawing without explanatory text
cannot earn full credit.
Lecture, textbook and other appropriate information ought to be integrated 5 points for grammar,
clarity of writing, spelling of technical terms, and other considerations.
Topic I. Compare and contrast different approaches and methods that humans use for controlling the growth and
survival of microorganisms. A thorough response will provide indications of how growth and survival are
quantified.
Topic II. In the book
Arrowsmith,
Sinclair Lewis describes the attempts of Dr. Arrowsmith and others to control
bacterial infections in humans. Interestingly, one type of control agent he utilized acted in some ways as an arrow
directed against the infections. What were the pathogens? What was that "arrow"? What effect did it have upon
the pathogens? Choose one type of "arrow", lytic or lysogenic, and make a recommendation to the reader why
that type, and not the other type, would be most appropriate for controlling infection.
A thorough answer will include some appropriate reference to the Lewis book
and
also refer to a current research
front in infectious disease control that is closely related to this subject in the book.
B. MULTIPLE CHOICE QUESTIONS.
Choose the letter of the
best
response and darken the corresponding
circle on your Scantron Sheet (Use Number 2 Pencil!) No extra time will be given to fill in the circles. You may
write on these pages, but only the responses on the Scantron Sheet will be graded.
1.
A microorganism which grows in a functioning refrigerator would be a(n):
A. thermophile
B. mesophilepsychrophile D. hyperthermophile
E. endospore
2.
In which growth phase(s) does cell number stay approximately the same over time?,,,.
A. Lag
B. Exponential
C. Stationary
D. Death
;’iE. two of the above
Which of the following forms of oxygen is generally toxic to living organisms?
A Superoxide ion
B Hydrogen Peroxide
C Hydroxyl Radical
t
D All of the above
4. A strict anaerobe:
A. absolutely requires molecular oxygen for growth
B. is killed by molecular oxygen
C. is not affected by molecular oxygen either for growth 6isurvival
D. requires molecular oxygen at very low concentrations for the cell to be able to grow
M3813 Exam 2
pf3
pf4
pf5

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Mbio 3813 Name Lecture Exam 2 26 March 2009 Last 4 of ID No. INSTRUCTIONS: Write Name and Last four digits of ID No. on pp. 1 and 2 of exam. SCANTRON: Last Name, space, First Name (^) in NAME BOX and Last 4 digits of ID in IDENTIFICATION NUMBER BOX. Fill in the circles before time runs out. No extra time will be given! All books and notes must be closed. No electronic devices of any kind may be used Cell Phone Alert: (^) Ifyour cell phone is heard ringing during the exam, there is a mandatory 5 (’five) point penalti’. Ifyou expect a very important call, identify yourself to the instructor for special arrangements. Academic Misconduct. Submission of the exam indicates tacit agreement with the statement "I have neither given nor received inappropriate aid in completing this exam." Using crib sheets, electronic communication, looking at another person’s paper, or any other activities which compromise the integrity of the exam are unacceptable and will be dealt with.

A. ESSAY QUESTION (20pIs): Choose one topic from the following list. Write the Roman Numeral in the small box on p. 1. Write your answer to the topic in the large box. No material outside the big box will be considered or graded Please write legibly! Criteria for essay: 15 points for accuracy and thoroughness of answer. A (^) list, table, or drawing without explanatory text cannot earn full credit. (^) Lecture, textbook and other appropriate information ought to be integrated 5 points for grammar, clarity of writing, spelling of technical terms, and other considerations.

Topic I. Compare and contrast different approaches and methods that humans use for controlling the growth and survival of microorganisms. A thorough response will provide indications of how growth and survival are quantified.

Topic II. In the book Arrowsmith, (^) Sinclair Lewis describes the attempts of Dr. Arrowsmith and others to control bacterial infections in humans. Interestingly, one type of control agent he utilized acted in some ways as an arrow directed against the infections. What were the pathogens? What was that "arrow"? What effect did it have upon the pathogens? Choose one type of "arrow", lytic or lysogenic, and make a recommendation to the reader why that type, and not the other type, would be most appropriate for controlling infection. A thorough answer will include some appropriate reference to the Lewis book and (^) also refer to a current research front in infectious disease control that is closely related to this subject in the book.

B. MULTIPLE CHOICE QUESTIONS. Choose the letter of the best response and darken the corresponding circle on your Scantron Sheet (Use Number 2 Pencil!) No extra time will be given to fill in the circles. You may write on these pages, but only the responses on the Scantron Sheet will be graded.

  1. A microorganism which grows in a functioning refrigerator would be a(n): A. thermophile (^) B. mesophilepsychrophile D. hyperthermophile (^) E. endospore
  2. In which growth phase(s) does cell number stay approximately the same over time?,,,. A. Lag B. Exponential C. Stationary D. Death (^) ;’iE. two of the above

Which of the following forms of oxygen is generally toxic to living organisms? A Superoxide ion B Hydrogen Peroxide (^) C Hydroxyl Radical t D All of the above

  1. A strict anaerobe: A. absolutely requires molecular oxygen for growth (^) B. is killed by molecular oxygen C. is not affected by molecular oxygen either for growth 6isurvival D. requires molecular oxygen at very low concentrations for the cell to be able to grow

M3813 Exam 2

  1. When bacteriophage lambda infects a cell which is susceptible to infection by this phage, which of the following events will occur? A. the cell will lyse (^) B. the cell will incorporate phage DNA into the cell’s chromosome C. neither of the above two events will occur D. one or the other of the two events will occur
  2. Comparing two cultures: the culture with the slower exponential growth rate has a: generation time than does the other. A. longer B. shorter^ C. same^ D. this is impossible to determine
  3. Pasteurization is a process which liquids. A. sterilizes but does not alter the flavors and food properties of B. sterilizes but alters the flavors and food properties of C. kills pathogens in D. leads to fermentation of organic compounds in the

The antimicrobial effectiveness of which of the following is limited to exposed surfaces? A. Microwaves B. Ultraviolet rays C. X-rays and gamma rays D. electrons

  1. Microbial cells in culture, treated with a known bacteriolytic agent, would demonstrate a(n):________________ A. increase in the turbidity of the medium. B. ecrease in the turbidity of the medium C. no change in the turbidity of the medium, but a ecrease in the viable count of the culture D. increase in the viable count of the culture
  2. Here is the sequence of a double stranded segment of DNA: GGATCC CCTAGG

This sequence is an example’of a:_______________ A. promoter B. RNA Gene C: palindrome D. codon^ E. restriction enzyme

  1. Plasmids can be found in: A. bacteria B. archaea C. bacteria and archaea D. plasmids are not stable and are lost when cells grow
  2. When successful PCR is performed in the labora, y, the following is the result:____________ A. RNA is synthesized B.DNA molecules are synthesized C. a sterile object or surface is obtained ’D Protein Copies of RNA sequences E. the sequence of a DNA molecule is obtained
  3. It is now well-recognized that cells in microbial populations communicate with one another. The mechanism of communication is called:___________________ A. conjugation B. respiration C. quorum sensing D. chemotaxis response system E. induction

I 4.2Pr9teomics is research approach to study:_________________ I Avhat genes are expressed under certain growth conditions B. antibiotic effects on protein synthesis C. structures of chromosomes D. plasmid replication mechanisms

15. A microorganism which grows in environments which have low nutrient concentrations is called a(n)________ A. anaerobe B. oligotroph C. fastidious microbe D. eutroph

M3813 Exam 2 (^3)

wo (^) .. .: (^) - .:

  1. A disinfectant compound is used to:________________ A. Remove potentially pathogenic microorganisms from the skin B. Kill all microorganisms on a inanimate surface or in a space C. Kill pathogenic microorganisms on an inaminate surface or object D. Purify foodstuffs with regard to spoilage microorganisms

254n antibiotic resistance gene provides selective advantage to a bacterial cell: when the organism is exposed to the antibiotic BT’ when the organism is not exposed to the antibiotic C. it does not provide selective advantage to a bacterial cell D. by permitting the organism to use the antibiotic as a carbon source

  1. The time required for a 10-fold reduction in the viable count of a population of microorganisms at a given temperature is called the:_______________ A. mean sterilization time B. decimal reduction time C. thermal death time D. temperature-dependent logarithmic death time
  2. 2 02.-+ 2 H - H202 + 02 depicts the reaction of: A. photosynthesis, light-dependent system B. catalase C. superoxide dismutase D. superoxide dismutase plus catalase E. respiration, terminal electron accepting reaction

2&-,;-An obligate anaerobe: A. cannot grow in the presence of 02 B. requires the presence of (^02) C. Neither requires nor is inhibited by the presence of 02 D. requires minute concentrations of 02

  1. A plaque formed on a lawn of bacterial cells on an agar plate is an indicator of:_________ A. a mutation B. antibiotic resistance C. bacterial mating^ D. bacteriophage infection

3k TP is a Wnucleoside triphosphate B. deoxynucleoside triphosphate C. dideoxynucleoside triphosphate D. codon E. antibiotic

  1. At a given temperature, the time required for a 10-fold reduction in the viable count of a population of vegetative cells is going to be: the time required for a 10-fold reduction of viable count for a population of endospores of the same microorganism. A. longer than B. the same as C. shorter than D. this can not be determined, since endospores are heat-stable
  2. A person asks the question, "when I set about sequencing a bacterial genome, about how many base pairs (order of magnitude) will be found-in the completed sequence?" The answer is:__________ A. 1000 B. 10,000 (C.P000000 D. 1 x 1012^ E. 1027

¶ 33. One studies thegrowth of a particular strain of bacterium. When cells are inoculated into a flask of medium and measurements are made over time, the system itself can be considered to be a(n):__________ A. batch culture B. continuous culture C. growth curve D. exponential growth curve

  1. A bacteriostatic agent has the following effect on bacteria affected by it:_________ A. it decreases the viable count of bacteria B. it decreases the total count of bacteria C. it decreases the viable count and total count of bacteria D. it prevents an increase in viable count and total count of bacteria but does not decrease these counts

M3813 Exam 2 (^5)

  1. True or False: Bacterial cells do not differentiate. A. True B. False
  2. The equation N = N O 211 describes the:_____________________ A. time it takes for a culture to enter stationary phase. complete growth curve for a batch culture in the laboratory C. increase in number of cells during exponential growth period for a culture D. effect of a bacteriocidal compound on a culture treated for time t
  3. Treat a healthy culture of bacteria growing in medium as follows. Add small amounts of acid every 5. minutes such that the pH of the medium decreases by 0.2 pH units after each addition. After 25 minutes (1.0 pH unit decrease in the medium), the cytoplasmic pH of the bacterium will:__________________ A. decrease by 1.0 pH unit B. increase by 1.0 pH unit C. remain essentially unchanged because of pH homeostasis mechanisms D. increase by 2.0 pH units to offset the external pH change
  4. In a bacterial cell a set of genes related by function, distributed at various sites around the chromosome can be controlled by a single response mechanism. The set of genes under this control is called

eguion B. operon C. promoter D. structural gene

  1. The set of genes described in Question 34 can be up-regulated under a certain type of environmental condition (e.g. change in nutrients, temperature, or many others). The genes that are up-regulated are said to be: A. mutated B. repressed^ C. induced^ D. polycistronic^ E. uncontrolled
  2. An enzyme involved in DNA replication in E.^ jj called Topoisomerase type II, utilizes ATP to DNA. - A. relax supercoils in. "B. introduce supercoils into C. synthesize RNA primers f the replication of D. initiate the replication process of cellular
  3. The terms germination, vegetative growth, exosporangiurn, dipicolinic acid are all associated with:. A. bacteriophage life cycles B. antibiotic function /C. endospores D. DNA replication

M38l3 Exam 2 (^6)