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Exam 1 solved - General Microbiology | BIO 308, Exams of Biology

University of Kentucky (UK)Biology
Prof. S. M. Steiner

Material Type: Exam; Professor: Steiner; Class: GENERAL MICROBIOLOGY; Subject: Biology; University: University of Kentucky; Term: Fall 2012;

Typology: Exams

2012/2013

Uploaded on 02/20/2013

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Examination #1 Bio 308-General Microbiology—Fall 2012 Steiner
If there appears to be more than one answer, choose the BEST answer.
1. The individual who is credited with identifying white blood cells as being phagocytic is:
A. Robert Koch
B. Louis Pasteur
C. Ignaz Semmelweis
D. Elie Mechnikoff*
E. Thomas Hunt Morgan
2. Which of the above scientists (A-E) is credited with pioneering the use of aseptic techniques to avoid
sepsis?
A. B. C.* D. E.
3. Which of the above scientists (Question 1-A-E) was awarded the first Noble Prize in what would
become Molecular Biology - Molecular Genetics?
A. B. C. D. E.*
4. Which of the individuals below received a Nobel prize for working out the elements of the innate
immune system?
A. Smith Nathans and Arber
B. Mellow and Fire
C. Capecchi, Evens and Smithies
D. Beutler, Hoffman and Steinman*
5. Which of the individuals listed below is associated with the development of penicillin as a systemic
antimicrobial treatment in the late 1930's- early 1940's after reading Flemings 1928 publication about
penicillin inhibiting bacterial growth on a petri plate?
A Lister
B. Domagk
C. Chain*
D. Pasteur
6. Arachidonic acid (shown below), the precursor of prostaglandins and leukotrienes is a good example of
a(an):
A. omega 3 fatty acid
B. polyunsaturated fatty acid*
C. steroid
D. straight chain fatty acid
1
pf3
pf4
pf5
pf8
pf9
pfa

Partial preview of the text

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Examination #1 Bio 308-General Microbiology—Fall 2012 Steiner If there appears to be more than one answer, choose the BEST answer.

  1. The individual who is credited with identifying white blood cells as being phagocytic is: A. Robert Koch B. Louis Pasteur C. Ignaz Semmelweis D. Elie Mechnikoff* E. Thomas Hunt Morgan
  2. Which of the above scientists (A-E) is credited with pioneering the use of aseptic techniques to avoid sepsis? A. B. C.* D. E.
  3. Which of the above scientists (Question 1-A-E) was awarded the first Noble Prize in what would become Molecular Biology - Molecular Genetics? A. B. C. D. E.*
  4. Which of the individuals below received a Nobel prize for working out the elements of the innate immune system? A. Smith Nathans and Arber B. Mellow and Fire C. Capecchi, Evens and Smithies D. Beutler, Hoffman and Steinman*
  5. Which of the individuals listed below is associated with the development of penicillin as a systemic antimicrobial treatment in the late 1930's- early 1940's after reading Flemings 1928 publication about penicillin inhibiting bacterial growth on a petri plate? A Lister B. Domagk C. Chain* D. Pasteur
  6. Arachidonic acid (shown below), the precursor of prostaglandins and leukotrienes is a good example of a(an): A. omega 3 fatty acid B. polyunsaturated fatty acid* C. steroid D. straight chain fatty acid
  1. Which of the class of compounds shown below, when added to one’s diet, markedly increases the risk of coronary heart disease. A B C D. none of the above*
  2. The backbone of DNA contains phosphates. These phosphates contain a negative charge and therefore which of the amino acids (as part of a protein) shown below is likely to have an ionic interaction with the DNA?

A B C D*

  1. The figure below best shows: A. Gram negative peptidoglycan B Gram positive peptidoglycan* C. either Gram positive or Gram negative peptidoglycan D. acid fast specific structure
  2. The structure below represents part of a(an) ____________ organism A. Gram negative* B. Gram positive C. Acid fast D. too little information to determine which of the above
  3. If you constructed a mutant of Mycobacterium tuberculosis that did not have lipoarabinomannan (LAM) you would expect that mutant to (choose the BEST based on information given during lecture and not on speculation ) : A. stain more readily B. grow faster C. be less virulent* D. be more readily killed by cell wall antibiotics
  1. Based on the video we watched in class, one hypothesis for how flagella rotate involves protons (H+) interacting with ___________ in a flagellar protein. A. lysine B. arginine C. aspartic acid * D. histidine
  2. Without __________ Neisseria gonorrhea are not virulent because they are unable to attach. A. flagella B. pili* C. capsules D. slime layer
  3. Which of the above structures (18 A-D) is of greatest importance in the process of tooth decay? A. B. C. D.*
  4. Which of the plates in the figure below would be the best one to use for direct counting of the number of bacteria assuming that you had a large number of plates to count? A. the top plate because it has the most colonies and therefore is likely, from a statistical point of view, to yield the most significant results B. The middle plate because there are sufficient numbers of colonies to yield significant results and the colonies are well separated and can readily be counted using a colony * C. the bottom plate because the colonies are so well separated D. any of the 3 plates, it really does not make a difference.
  1. The spore coat is composed in large part of ____________. A. dipicolinic acid B. peptidoglycan C. a keratin like protein* D. membrane glycoproteins
  2. A mutant bacterium that has a defective sliding clamp will not be able to replicate its DNA very well because: A. Okazaki fragements cannot be made because the RNA primase will likely fall off of the replication fork B the DNA polymerase will likely fall off of the template before it is done making a DNA copy* C. the single stranded binding proteins are unable to bind to the DNA and therefore DNA replication is blocked D. In fact an organism that has a defective sliding clamp can replicate DNA as well as a wild type organism
  3. During DNA replication: A. the leading strand is synthesized in the 3' to 5' direction B. the leading strand is synthesized discontinuously C. the lagging strand is synthesized in the 5' to 3' direction* D. both strands are synthesized discontinuously
  4. Which of the following statements about transcription is NOT accurate? A. only one strand can act as an RNA template for any particular gene B. RNA polymerase moves in a 5' to 3' direction C. the sigma factor acts as a helicase in the initiation of mRNA synthesis in both pro- and eukaryotic cells* D. All of the above statements are accurate (if you believe that all of the above statements are accurate, choose this answer).
  5. Which of the following statements is NOT accurate? A. In prokaryotic cells one mRNA molecule can transcribe more than one gene B. In both pro- and eukaryotic genes RNA polymerase binds to the promoter region C. In eukaryotic cells one mRNA can transcribe more than one protein by alternative splicing D. All of the above statements are accurate (if you believe that all of the above statements are accurate choose this answer).*
  6. Which of the following statements about translation is NOT accurate? A. The start codon is the first codon in the mRNA and encodes for formyl-methionine in prokaryotes B. Amino acids are linked to tRNA by an enzyme (aminoacyl tRNA synthetase) that recognizes the anticodon of the tRNA C. codons of eukaryotic versus prokaryotic cells differ significantly* D. the last codon during the transcription of a molecule of mRNA is a stop codon.
  1. The figure below illustrates catabolite repression. This figure indicates the growth of E. coli grown in medium containing glucose and lactose. The lactose is not utilized until all of the glucose is used up. The reason for this is ( select the BEST answer) : A. initiation of the lac operon requires binding of the glucose to the lac repressor B. the presence of glucose causes the level of cyclic AMP to be low* C. when glucose is metabolized some of the glucose metabolites block transport of lactose D. when glucose is being metabolized the pH in the cytoplasm is too low to activate the lac operon
  2. Luria and Delbruck's fluctuation test supported the hypothesis that mutations conferring antibiotic resistance: A. are produced by exposure to the antibiotic B. are random and are not produced by exposure to the antibiotic* C. vary among different types of bacteria D. are mostly frameshift types of mutation
  3. Which of the following statements about the Ames test is NOT accurate? A. The purpose of the microsomal fraction in the Ames test is as a nutritional supplement for the strain of Salmonella typhimurium (S. typhi) used in the test.* B. the Ames test is based on a gain of function mutation (gain of the ability to grow in the absence of histidine in the medium) C. The Ames test is a test for mutagens not for carcinogens D. The Ames test is the first line test in commercial products for determining whether or not a product is potentially carcinogenic
  1. A recent study reported that ________________ can reduce sinus problems A. certain antibiotics B. bacteriophage C. the right mixture of bacteria* D. injection of testosterone into the sinus region
  2. Taking high doses of Vitamin __ appears to speed the recovery from tuberculosis A. A B. B C. C D. D* E. E
  3. A ______________ improves thinking in monkeys. A. steroid hormone B. brain implant* C. electrotherapy to the brain D. high levels of B vitamins E. very low carbohydrate diet