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Class: BIOL 4004 - Cell Biology; Subject: Biology; University: University of Minnesota - Twin Cities; Term: Spring 2013;
Typology: Quizzes
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TERM 1 DEFINITION 1 the inject door nucleus can direct the recipient egg to produce normal tadpole, which is basically cloning tadpole has full range of differentiated cells obtained from original donor cell TERM 2 DEFINITION 2 exposing of liver cell to a glucocorticoid hormone, for example,the production of several specific proteins is dramatically increase Glucocorticoids release increase in the body during starvation and crease the liver to produce glucose So, the human brain and liver cells have different pattern of protein TERM 3 DEFINITION 3 a cell can control the proteins it makes by controlling six steps show in the figure TERM 4 DEFINITION 4 gel- mobility shift assay: allows even trace amounts of a sequence-specificDNA- bindingproteinbe readily detected short DNA fragment of specific length labeled and mixed with cells. free DNA move fast loaded onto a polyacrylamide gel and subjected to electrophoresis It is native gel and protein would bind TERM 5 DEFINITION 5 once a sequence-specific DNA protein has been purified, the gel-mobility shift assay can be used to study the strength and specificity of its interactions with different DNA sequences
TERM 6
DEFINITION 6 is a protein that increases gene transcription of a gene or set of genes. the bindchromatinin the promoter region recruitRNA polymerase TERM 7 DEFINITION 7 used once the DNA sequence that a gene regulatory protein recognizes has been determine double-stranded oligonucleotide of the correct sequence is synthesized by chemical methods and linked to an insoluble porous matrix such as agarose attach beads to the determined DNA sequences ion interaction between the activator and repressor DNA TERM 8 DEFINITION 8 determining the sites on DNA that a given gene regulatory protein occupies proteins are covalently cross-linked to DNA in living cells cells are broken open, and the DNA is mechanically sheared into small fragments TERM 9 DEFINITION 9 Antibodies directed against a given gene regulatory protein are then used to purify DNA reversecross-linkeddenature DNA Used theremoved(isolated DNA) in PCR TERM 10 DEFINITION 10 promoter, where the general transcription factors and the polymerase assemble regulatory sequences to which gene regulatory proteins bind to control the rate of the assembly processes at the promoter