TERM 1 DEFINITION 1
double helix must be opened up and the two strands
separated to expose unpaired bases
positions at which the DNA helix is first opened are called
replication origins
TERM 2 DEFINITION 2
DNA rich inA-Tbase pairs is relatively easy to pull apart
regions of DNA enriched in A-T pairs are typically found at
replication origins.
TERM 3 DEFINITION 3
In these experiments, cells were firs t grown in the presence of
BrdU (a thymidine analog) and absenc e of thymidine to label
the DNA uniform
Cells were briefly pulsed with thymidin e in the absence of BrdU
during the early, Middle, or late S phas e
DNA made during the thymidine puls e is a double helix with
thymidine on one strand and BrdU on the other
the one has BrdU on both strands sta in darker
Negative one has bright band
TERM 4 DEFINITION 4
population of cells is sychronizes dot h at they all begin
replication at the same time
DNA is collected and hybridized to the microarray DNA that
has been replicate do negatives a hy bridization signal(dark
green squares) twice as high as that of unreplicated DNA(light
green squares
the replication begins at an origin and proceeds bidirectionally
In yeast replication start at hundreds oforigins
TERM 5 DEFINITION 5
If a circular bacterial plasmid contain a gene is introduce into
the mutant yeast directly, the plasm id will be able to replicate,
because it lacks a functional origin
If random pieces of yeast DNA are ins erted into this plasmid,
only the rare plasmid DNA that conta in the origin relplicate
A DNA sequence identified by its pres ence in a plasmid
isolated from these surviving yeast ce lls is called ARS
ARS is chromosomal origins of replicati on
