TERM 1 DEFINITION 1
Leadingstrand: allows the new ly synthesized strand
complementary to the original strand to be synthesized 5' to 3'
in the same direction as the movem ent of the replication fork.
Lagging strand: opposite t o the working orientation of DNA
polymerase III, which moves on a tem plate in a 3' to 5'
manner, replication of the lagging stra nd is more complicated
than that of the leading strand.
TERM 2
exonucleolytic proofreading
DEFINITION 2
clips off any unpaired residues at the primer terminus,
continuing until enough nucleotides have been removed
to regenerate a correctly base-paired 3'-oH terminus that
can prime DNA snthesis
TERM 3 DEFINITION 3
This schematic diagram shows a current view of the
arrangement of replication protein sat a replication fork when
DNA is being synthesized
TERM 4
RNA polymerase
DEFINITION 4
catalyze the formation of the phosphodiester bonds that
link the nucleotides together to form linear
unwind the DNA helix
RNA polymerases can start an RNA chain without a
primer.
TERM 5 DEFINITION 5
(B) An electron micrograph showing t he replication machine
from the bacteriophageT4 sit moves along template
synthesizing DNA behind it.
(C) An interpretation of the micrograp h is given in the sketch:
note especially the DNA loop on the lagging strand Apparently
the replication proteins became part ly detached from the very
front of the replication fork
