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Eukaryotic Cell, Electron Microscope, Focusing Is Achieved, Image Is Detected, Negative Staining, Different Staining Techniques, Schematic Layout, Phosphatidylcholine Molecule are some points from questions of Biology of the Cell subject.
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Answer all multiple choice questions on one optically marked sheet. You must answer each question in the order in which it appears on the question paper.
Complete the boxes on the optically marked sheet to give the following details - name, date, subject, module identifier and student number.
Student number should be completed by writing the digits in pencil in the top line of the box and then filling in the bar code boxes below with the first digit represented in column 1 , the second digit in column 2 and so on. This is done by striking through the little box with a soft pencil as shown in the instructions on the sheet for marking the correct answers. Other instructions are on the sheet, but note the following:-
There are five possible answers to each question, only one of them is correct.
Record your answers on the optically read sheet by using a soft pencil (HB or softer) to mark the appropriate box thus: Do not tick, cross or ring boxes and do not write elsewhere on the form.
No marks added/deducted for blank answers.
If you make a mistake on the optically marked sheet, it can be corrected with a soft eraser.
Spare sheets are available in the examination hall.
4. Read text B. This question asks you to replace an underlined letter in text B with a
word or phrase chosen from the list below. Which word or phrase would you choose to replace X?
a) Helical. b) Linear. c) Single-stranded. d) Double-stranded. e) Circular.
5. Read text B. This question asks you to replace an underlined letter in text B with a word or phrase chosen from the list below. Which word or phrase would you choose to replace Y?
a) Parasitic. b) Commensal. c) Endosymbiotic. d) Exocytotic. e) Endosomal.
6. Read text B. This question asks you to replace an underlined letter in text B with a
word or phrase chosen from the list below. Which word or phrase would you choose to replace Z?
a) Cyclohexamide. b) Colchicine. c) Caffeine. d) Chloramphenicol. e) Chloroquine.
Figure 1
7. Look at figure 1 which shows part of a eukaryotic cell. What is the name of structure
to which X is pointing?
a) The rough endoplasmic reticulum. b) The nuclear envelope. c) A lysosome. d) The Golgi complex. e) The smooth endoplasmic reticulum.
8. Look at figure 1 which shows part of a eukaryotic cell. What is the name of
membrane to which Y is pointing?
a) The rough endoplasmic reticulum. b) The nuclear envelope. c) A lysosome. d) The Golgi complex. e) The smooth endoplasmic reticulum.
9. Look at figure 1 which shows part of a eukaryotic cell. What is the name of
membrane to which Z is pointing?
a) The rough endoplasmic reticulum. b) The nuclear envelope. c) A lysosome. d) The Golgi complex. e) The smooth endoplasmic reticulum.
Text C. The protein motor X transports vesicle cargo the minus end of microtubule (which tend to be organized deep within a cell). It gains energy for this process by hydrolyzing Y. This protein also provides motive force in Z.
10. Read text C. This question asks you to replace an underlined letter in text C with a
word or phrase chosen from the list below. Which word or phrase would you choose to replace X?
a) Clathrin. b) Caveolin. c) Dynein. d) Kinesin. e) Transferrin.
11. Read text C. This question asks you to replace an underlined letter in text C with a
word or phrase chosen from the list below. Which word or phrase would you choose to replace Y? a) ATP. b) GTP. c) Glucose. d) H+. e) Sucrose.
16. Read text E. This question asks you to replace an underlined letter in text E with a number chosen from the list below. Which number would you choose to replace X?
a) 300. b) 600. c) 900. d) 1200. e) 1500.
17. Read text E. This question asks you to replace an underlined letter in text E with a word or phrase chosen from the list below. Which word or phrase would you choose to replace Y? a) Annexins b) Connexins c) Cadherins d) Integrins e) Occludins 18. Read text E. This question asks you to replace an underlined letter in text E with a word or phrase chosen from the list below. Which word or phrase would you choose to replace Z? a) Anion-specific b) Cation-specific c) Hydrophilic d) Hydrophobic e) Non-polar
Text F. p53 is often referred to as the ‘Guardian of the Genome’. In the event of DNA damage p53 X by preventing the cell from passing through Y. If the DNA damage is too severe, p53 can trigger programmed cell death (apoptosis) by inducing expression of PUMA a protein which blocks the anti-apoptotic protein Z.
19. Read text F. This question asks you to replace an underlined letter in text F with a word or phrase chosen from the list below. Which word or phrase would you choose to replace X? a) Accelerates mitosis. b) Blocks segregation. c) Halts the cell cycle. d) Slows the cell cycle. e) Triggers cytokinesis. 20. Read text F. This question asks you to replace an underlined letter in text F with a word or phrase chosen from the list below. Which word or phrase would you choose to replace Y? a) G1. b) Mitosis. c) The reaction point. d) The restriction point. e) The spindle checkpoint.
21. Read text F. This question asks you to replace an underlined letter in text F with a word or phrase chosen from the list below. Which word or phrase would you choose to replace Z? a) Apaf- b) Bax c) Bcl- d) Caspase e) Cohesin
Text G. Following translation proteins are often subjected to further processing. X are enzymes which cleave proteins by hydrolysing peptide bonds. In some cases this results in a form of protein splicing in which segments of polypeptide, called Y , are removed. Other modifications include the formation of disulphide bonds between Z residues catalysed by disulphide isomerase.
22. Read text G. This question asks you to replace an underlined letter in text G with a word or phrase chosen from the list below. Which word or phrase would you choose to replace X? a) Kinases. b) Oxidases. c) Phosphatases. d) Proteases. e) Synthases. 23. Read text G. This question asks you to replace an underlined letter in text G with a word or phrase chosen from the list below. Which word or phrase would you choose to replace Y? a) Exons. b) Inteins. c) Introns. d) Outeins. e) Outrons. 24. Read text G. This question asks you to replace an underlined letter in text G with a word or phrase chosen from the list below. Which word or phrase would you choose to replace Z? a) Leucine. b) Cysteine. c) Serine. d) Alanine. e) Methionine.
There are many different staining techniques for electron microscopy samples. Briefly state what you understand by the term negative staining or shadowing (3 marks).
Question 3.
Figure 2 (phosphatidylcholine) Sphingomyelin
Figure 2 shows a schematic layout of a phosphatidylcholine molecule (phosphatidylcholine is a phosphoglyceride). Two of the components are missing (X and Y). Give the names of these two components (2 marks). Draw a similar figure (in the space provided) to show the structure of sphingomyelin (a sphingolipid) labelling each component of the molecule. (4 marks)
Missing component X is…
Missing component Y is…
Question 4.
Sketch a graph showing the relationship between concentration of a solute (e.g. glucose) and the rate of its uptake into a cell by facilitated diffusion (2 marks). Label the graph axes (2 marks).
Briefly explain the shape of the graph (2 marks).
Question 5.
What is the basic structure of cellulose (1 marks).
How do hemicelluloses differ from cellulose? (2 marks)
Question 7.
Give one function of the mRNA cap (1 mark).
Draw a figure to show the main events in pre-mRNA splicing (5 marks).
Question 8.
Name of fibre
Major protein constituent
Name of fibre Name of fibre
Major protein constituent
Major protein constituent
Figure 3
Figure 3 shows the three main types of fibres in the cytoskeleton.Labelling the figure, give the name of each of these three types of fibres and name one major protein constituent of each type (4 marks).
In the context of the cytoskeleton, what are MAPS (1 mark).
Give an example of a MAP and state a function for your named example (1 mark).