Epitope Mapping Services using X-ray Crystallography by Creative Biostructure, Slides of Biology

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Epitope mapping using

crystallography

With skills and knowledge on co-crystal structures, Creative Biostructure provides epitope mapping services specializing in X-ray crystallography technique. Epitope mapping aims at identifying the binding sites of antibodies on their target antigens. It has become increasingly vital in discovery and development of new vaccines and antibody drugs. Epitope identification and characterization of an antibody will greatly facilitate drug discovery and design. Epitopes can be classified into two main groups: linear and conformational epitopes. A linear epitope is the continuous section of the sequence; a conformational epitope, on the other hand, is composed of discontinuous subsections of an antigen sequence. Previous studies about epitopes show that most of the antigen-antibody interactions depend on conformational epitopes.

Epitope mapping is the process of identifying and characterizing the minimum molecular structures recognized by immune system, mainly T and B cells. Most epitopes recognized by antibodies or B cells are three-dimensional surface features of a protein antigen; these features fit the complementarity determining regions (CDRs) precisely and thus are bound by antibodies. Understanding this structure recognition mechanism relies on determining the structure of the antibody-antigen complex. Once the structure is known, physical interactions between the antigen and antibody can be confirmed and depicted at the atomic level. Therefore, X-ray crystallography is considered to be the most unambiguous method for epitope mapping.

Based on high standard X-ray crystallography methods, Creative Biostructure offers comprehensive epitope mapping services for both conformational and linear epitopes with high accuracy. In addition, our protein analysis and high-throughput crystallization services will greatly facilitate the epitope mapping project as well. We have extensive experience in crystallizing antibody-antigen complexes and resolving their 3-D structures. In particular, we are able to map the epitopes of a large variety of antibody forms, including intact IgG, Fab, scFv, F(ab')2, diabody, minibody, minoantibody, tandem scFv and nanobody and affibody. Our services: Overlapping peptide library construction Binding activity test by ELISA/WB X-ray crystallography 3D-EM (Electron Microscope) LC-MS

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mapping-using-crystallography-88.htm