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Cell Membrane Proteins, Junctions, and Muscle Tissue: A Comprehensive Overview, Study Guides, Projects, Research of Communication

Epithelial tissues are physically separated from underlying connective tissues by a basement membrane or basal lamina. The portion of an epithelial cell.

Typology: Study Guides, Projects, Research

2021/2022

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Epithelial tissue (epithelium)
General characteristics of epithelium
Is avascular tissue (without blood supply cells receive nourishment by
diffusion from a highly vascular area of loose connective tissue just below
the basement membrane called the lamina propria )
is highly cellular tissue cells are arranged to form cohesive sheet or
groups with no or little extracellular matrix
displays a free surface usualy luminal surface (turned to the lumen)
opposite (basal) surface adheres to extracellular basement membrane or
lamina basalis
epithelial cells display polarity apical (luminal), lateral and basal
surfaces with structural specialization
epithelial cells are specialised for absorption, secretion or to act as barrier
lateral surfaces display junctional complexes for intercellular cohesion
and communication
One type of epithelium may change into another type metaplasia (examples:
pseudostratified ep. of respiratory passages transforms into stratified squamous
ep. on the surface of epiglottis and soft palate)
Membrane specializations of epithelia
Lateral surface
Specialised structures are present in epithelia which link individual cells together. Two
main adhesion types are distinguished:
1. Cell membrane proteins acting as specialised cell adhesion molecules (CAMs)
2. Specialised areas of the cell membrane incorporated into cell junctions.
Three types are recognized: occluding junctions, anchoring or adherence junctions and
communicating junctions.
o Occluding junctions bind cell together to form an impermeable barrier
Zonula occludens or tight junction
o Anchoring junctions link the cytoskeleton of cells to each other and two
underlying tissues
Zonula adherens provides mechanical strength
Macula adherens or desmosomes provides mechanical strength in
tissues where there are tensile or shearing stresses, eg skin
o Communications junctions allow direct cell-cell communication
Gap junction or nexus allow rapid communication for
coordinated action
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Epithelial tissue (epithelium)

General characteristics of epithelium

Is avascular tissue (without blood supply – cells receive nourishment by

diffusion from a highly vascular area of loose connective tissue just below

the basement membrane called the lamina propria )

is highly cellular tissue – cells are arranged to form cohesive sheet or

groups with no or little extracellular matrix

displays a free surface – usualy luminal surface (turned to the lumen)

opposite (basal) surface adheres to extracellular basement membrane or

lamina basalis

epithelial cells display polarity – apical (luminal), lateral and basal

surfaces with structural specialization

epithelial cells are specialised for absorption, secretion or to act as barrier

lateral surfaces display junctional complexes for intercellular cohesion

and communication

One type of epithelium may change into another type – metaplasia ( examples:

pseudostratified ep. of respiratory passages transforms into stratified squamous

ep. on the surface of epiglottis and soft palate )

Membrane specializations of epithelia

Lateral surface

Specialised structures are present in epithelia which link individual cells together. Two

main adhesion types are distinguished:

1. Cell membrane proteins acting as specialised cell adhesion molecules (CAMs)

2. Specialised areas of the cell membrane incorporated into cell junctions.

Three types are recognized: occluding junctions, anchoring or adherence junctions and

communicating junctions.

o Occluding junctions bind cell together to form an impermeable barrier

 Zonula occludens or tight junction

o Anchoring junctions link the cytoskeleton of cells to each other and two

underlying tissues

 Zonula adherens provides mechanical strength

 Macula adherens or desmosomes provides mechanical strength in

tissues where there are tensile or shearing stresses, eg skin

o Communications junctions allow direct cell-cell communication

 Gap junction or nexus allow rapid communication for

coordinated action

Luminal (free, apical) surface

Microvilli – short finger-like projection of the cell membrane to increased

surface area (regularly arranged microvilli in intestines – striated border , in

kidney tubules – brush border )

Cilia – hair-like surface projections of cells involved in transport

Glycocalyx – thin extracellular layer consisting of protein glycoprotein and

sugar residues; stains PAS positive; can act as enzyme, CAM or for cell

recognition

Basal surface

Basal invaginations or folds – greatly enhance surface area; folded membrane with

ions pumps + mitochondria form basal labyrinth in kidney tubules.

Basal lamina – basement membrane

Epithelial tissues are physically separated from underlying connective tissues by

a basement membrane or basal lamina. The portion of an epithelial cell

attached to the basement membrane is called its basal surface. The opposite side

  • facing the external environment, or lumen of a body cavity, is its apical

surface. Basement membranes are composed of a special type of collagen and a

substance called laminin (see below). The basement membrane helps epithelial

cells orient themselves in relation to other tissues. After epithelial injury (e.g., an

abrasion), the basement membrane serves as a scaffolding upon which new cells

attach themselves during healing.

Cassification of epithelia

I. surface epithelium – is 1 or more layers of cells arranged into sheet;

According to

number of layers

According to shape of cells in the outermost

layer

SURFACE

EPITHELIUM ^ simple layerd^ –^ squamous

  • cuboid
  • columnar
  • pseudostratified columnar

 stratified – squamous non-cornified (non-keratinized)

  • squamous cornified (keratinized)
  • columnar
  • transitional

Epithelia with special functions:

resorptive, sensory, respiratory, myoepithelial cells

glandular epithelium – multicellular epithelial structures that specialize in

synthesizing and secreting complex molecules.

CLASSIFICATION OF GLANDS

GLANDULAR

EPITHELIUM

 unicellular Single cells in coverinrg epithelium –

(Paneth cells, goblet cells,

enteroendocrine cells, Leydig cells)

 multicellular Accordin of mechanism of secretion

endocrine

exocrine – merocrine, apokrine,holocrine

According to loclalization

intraepithelial extraepithelial

According to arrangement of ducts

simple branched compound

According to type of secretory portions

tubular alveolar (acinar) tuboalveolar

According to product properties

mucous serous mixed

According of mechanism of secretion

endocrine – glands withou ducts, product is released into the blood through the

wall of capilareis

exocrine – secretory cells of exocrine glands release their products into ducts in

three different ways:

merocrine apocrine holocrine

  • membrane-bound secretory granules are moved to the apical surface where they coalesce with the membrane on to release the product.
    • the apical portions of cells are pinched off and lost during the secretory process.
      • secretory cell degenerates and as it breaks apart, the contents of the cell become the secretory product.

According to

type of

secretory

units

to product

properties simple branched compound

tubular are usually

mucous

alveolar

(acinar)

are usually

serous

tuboalveolar mixed

Secretory units

Serous acinus (alveolus) Mucous tubule Serous demilune (Giannuzzi)

Functions of epithelia:

Barrier: Epithelial tissue commonly functions as a covering or lining for

organs and other tissues (e.g., skin, mucous membranes, pleural cavity,

etc.). Epithelial cells serve as selective barriers between the environment

and the internal structures of the body. They protect underlying tissues

from drying, and from mechanical and chemical injury. Tight junctions

between individual cells play an important role in the barrier function of

epithelium. Some barrier epithelial cells have motile cilia that propel fluid

or particulate matter over tissue surfaces (e.g., cells lining the bronchi).

Absorption: Epithelial cells are found in those organs (e.g., intestine)

which are involved in absorption of substances important for life. These

cells often microvilli which increase cell surface area in order to facilitate

absorption.

Secretion: The secretory cells of endocrine and exocrine glands are

epithelia.

  • skeletal muscle
  • cardiac muscle
  • smooth muscle

The fibres of skeletal muscle and cardiomyocytes (cells of cardiac muscle)

exhibit cross striations at the light microscope level and they are both referred to

as striated muscle.

Terms:

Plasmamembrane of muscle cells – sarcolemma

Cytoplasm of muscle cells – sarcoplasm

Smooth endoplasmic reticulum – sarcoplasmic reticulum

Muscles consist of muscle cells and connective tissue:

Muscle cells

Rhabdomyocyte

Cardiomyocyte

Leiomyocyte

Connective tissue

Connective tissue surrounds muscle fibres. Individual muscle fibres are

surrounded by a delicate layer of reticular fibres called the endomysium.

Groups of fibres are bundled into fascicles by a thicker CT layer called the

perimysium. The collection of fascicles that constitutes one muscle is

surrounded by a sheath of dense CT called the epimysium , which continues into

the tendon. Blood vessels and nerves are found in the CT associated with

muscle. The endomysium contains only capillaries and the finest nerve

branches. All three layers merge together at end of a gross muscle to form a

tendon.

Three basic layers

o endomysium - surrounds each cell (fiber)

o perimysium - surrounds a group of cells forms a fascicle

o epimysium - surrounds entire gross muscle

Endomysium

o thin delicate connective tissue

o blends with muscle cell membrane

Perimysium

o ordinary loose connective tissue

o divides groups of muscle cells into bundles within the gross

muscle

Epimysium

o capsule = dense irregular connective tissue

I. Skeletal Muscle

General Features

Called skeletal muscles or somatic (body) musculature

Rapid contractions

Voluntary innervation

Striations visible with light microscope (LM)

Skeletal muscle is attached to the skeleton and controls motor movements and posture. There are a few instances where this type of muscle is restricted to soft tissues: the tongue, pharynx, diaphragm and upper part of the esophagus.

Skeletal muscle fiber structure

Striated skeletal muscle cells = muscle fibers = a multinucleated syncytium

formed by the fusion of individual small muscle cells precursors – myoblasts,

during development.

Striated skeletal muscle cell = fiber (syncytium)

Cylindrical with tapered ends

Length – several cm, thickness - about 10-100 μm in diameter

II. Cardiacl Muscle

General Features

Wall of heart

Contracts rapidly

Autonomic (involuntary) innervation - cardiac muscle is regulated

by autonomic and hormonal stimuli

Lacks residual stem cells and therefore cannot regenerate after

damage

Cardiac muscle exhibits striations because it also has actin and

myosin myofilaments arranged into sarcomeres. Generally these

striations do not appear as well-defined as in skeletal muscle.

Cardiac muscle cells = cardiomyocytes

  • Cells are columnar, the end(s) can be branched
  • Cells are end-to-end arranged into fibers
  • Fibers branch and anastomose
  • Moderate length, about 100 μm
  • Moderate diameter, 10-50 μm
  • Striation is present and visible with LM
  • Single nucleus (rarely 2 nuclei) - centrally placed
  • Intercalated discs join ends of cells together –

desmosomes and gap junctions (nexuses)

A number of features distinguish cardiac from skeletal muscle:

  • cardiac muscle cells have only one or two nuclei, which are centrally located
  • myofibrils separate to pass around the nucleus, leaving a perinuclear clear area
  • 1 T-tubule + 1 terminal cisterna of sarcoplasmic reticulum = DIAD et the level

of border between A and I band , there are no triads (1 T-tubule – 2 cisternae)

as in skeletal muscle fibers

As in skeletal muscle, individual muscle fibres are surrounded by delicate

connective tissue. Numerous capillaries of coronary circulation are found in the

connective tissue around cardiac muscle fibres.

Cardiac muscle cells are joined to one another in a linear array. The boundary

between two cells abutting one another is called an intercalated disc.

Intercalated discs consist of several types of cells junctions whose purpose is to

facilitate the passage of an electrical impulse from cell to cell and to keep the

cells bound together during constant contractile activity.

Specialized fibres, called Purkinje fibres , arise from the atrioventricular node

and travel along the interventricular septum toward the apex of the heart,

sending branches into the ventricular tissue. Purkinje fibres are of larger

diameter than ordinary cardiac fibres, with fewer myofibrils and an extensive,

well-defined clear area around the nucleus. They conduct impulses at a rate

about four times faster than that of ordinary cardiac fibres and serve to

coordinate the contraction of the atria and ventricles.

III. Smooth Muscle

General Features

Walls of hollow viscera

Contracts slowly

often prolonged sustained contractions

Autonomic (involuntary) innervation

Smooth muscle is the intrinsic muscle of the internal organs and blood vessels. It

is also found in the iris and ciliary body of the eye and associated with hair

follicles (arrector pili). No striations are present in smooth muscle due to the

different arrangement of actin and myosin filaments. Like cardiac muscle,

smooth muscle fibres are intrinsically contractile but responsive to autonomic

and hormonal stimuli. They are specialized for slow, prolonged contraction.

Each csll is fusiform in shape with a thicker central portion and tapered at both

ends. The single nucleus is located in the central part of the cell. Cells range

enormously in size, from 20 (in wall of small blood vessels) to 500 (in wall of

uterus during pregnancy) micrometers. Smooth muscle cells lie over one another

in a staggered fashion (tapered part of one cell over thicker part of another).

One distinguishing physiological feature of smooth muscle is its ability to secrete connective tissue matrix. In the walls of blood vessels and the uterus in particular, smooth muscle fibres secrete large amounts of collagen and elastin.

Smooth muscle cells = leiomyocytes

  • Spindle shape cells - elongated and tapered
  • Moderate length - about 100-200 μm
  • Thin diameter - about 5-10 μm
  • Single nucleus - centrally placed
  • NO striations – aktin and myosin myofilaments are not arranged into

Nervous Tissue ( NT )

  • highly specialized tissue
  • forms, receives and sorts signals (irritability)
  • transmits electrical impulses (conductivity)

Functions of Nerve Tissue

Nervous tissue allows an organism to sense stimuli in both the internal and external environment. The stimuli are analysed and integrated to provide appropriate, co-ordinated responses in various organs. The afferent or sensory neurons conduct nerve impulses from the sense organs and receptors to the central nervous system. Internuncial or connector neurons supply the connection between the afferent and efferent neurons as well as different parts of the central nervous system. Efferent or somatic motor neurons transmit the impulse from the central nervous system to a muscle (the effector organ) which then react to the initial stimulus. Autonomic motor or efferent neurons transmit impulses to the involuntary muscles and glands.

  • NT forms central and peripheral nerve system (CNS and PNS)
  • NT consists of nerve cells = NEURONS and associated supporting cells = NEUROGLIA; neurons are specifically designed to transmit electrical impulses and to receive and process information; neuroglial cells are non-conducting cells that are in intimate physical contact with neurons. They provide physical support, electrical insulation and metabolic exchange with the vascular system.
  • NT originates from ectoderm

NEURON

Nerve cells are very variable in appearance, shape and size, but all neurons have a cell body, also called soma or perikarion , and processes extending from the nerve cell to communicate with other cells. There are two types of processes: dendrites that receive impulses and axons (neurits) that transmit impulses. All nerve cells have one axon, which is usually the longest process that extends from the cell and one or more (hundreds) dendrites, these are generally shorter and thicker than the axon. The junction where a nerve cell communicates with another nerve cell or an effector cell (eg. muscle fibre) is called a synapse, which can be chemical or electric. The terminal part of the axon with chemical synapses releases substances called a neurotransmitter which acts on the membrane of the other cell.

Cell body – PERIKARION: contains nucleus and most cytoplasm with organelles:

  • nucleus – round or oval, very light, with prominent nucleolus
  • rough ER (called Nissl´ substance) – involved in synthesis of proteins (neurotransmitters)
  • other usual organelles (mitochondria, Golgi apparatus, lysosomes)
  • neurofibrils – neurofilaments and neurotubules
  • pigment lipofuscin

DENDRITES – input structure – receive signals; number of dendrites: one – several

hundreds

short, branched processes with structure similar to perikarion (cytoplasm + organelles

  • neurofibrils) incoming signals summate to initiate action potential highly branched tree structure

Classification of neurons according to number of processes (dendrites):

  1. Multipolar neuron – several dendrites extend from body found in brain & spinal cord
  2. Bipolar neuron – one dendrite and one axon (in retina of eye)
  3. Unipolar neuron – one process only, link to axon (sensory neurons)
  4. Pseudounipolar neuron – one short process divides later into dendrite and axon (spinal ganglia)

AXON – only one

no protein synthesis here Trigger zone - where nerve impulses arise Axon hillock – the cone-shaped base of the axon, its cytoplasm is free of rER (Nissl substance) Axons terminal - end with fine branching with „terminal boutons“ – mitochondria and synaptic vesicles containing neurotransmitters Axon hillock and terminal are not covered with oligodendrocytes (in CNS) or Schwann cells (in PNS) Serves for impulses transmission and for axonal transport of neurotransmitters and nutrients

Classification of neurons according to length of axon:

  1. Golgi type I – long axon (up to 1 m) – somatic motor neurons
  2. Golgi type II - short axon (in μm)

Classification of neurons according to function:

  1. sensitive neurons – (afferent) conduct informations from receptors to CNS
  2. motor neurons – (efferent) conduct infirmations from CNS to effector cells: somatomotor to skeletal muscle and visceromotor to smooth muscle cells, cardiomyocytes or glandular cells
  3. interneurons (97 %)

Chemical Synapses

Presynaptic cell releases neurotransmitters from synaptic vesicles Act on the postsynaptic cell (help initiate AP) Neurotransmitters can excite or inhibit Neurotransmitters (acetylcholine, serotonin, norepinepherine and epinephrin, dopamine, GABA, …)

Neurotransmiter must be removed to prevent continual firing of neurons Enzymatically - acetylcholineresterase Many pharmaceuticals and drugs modulate this effect Cocaine block removal of dopamine

Electrical Synapses

Without synaptic vesicles; synaptic cleft – only 2 nm thick Depolarizating wave continues from presynaptic to postsynaptic membrane Morphologically (in electron microscope) it looks like communicatin intercellular connection: gap junction (nexus)

SUPPORT CELLS PLAY A VITAL ROLE

Support cells are essential to the function and survival of nerve cells. The CNS and PNS each have their own specific types of support cells.

Support cells in the CNS :

The general term for support cells in the CNS is glia or neuroglia (glial cells, neuroglial cells). There are four types of neuroglial cells. (1) Oligodendrocytes , the myelin-secreting cells of the CNS. (2) Astrocytes , which provide physical and metabolic support for nerve cells. (3) Microglia , (microglial cells), which are the phagocytes of the CNS. (4) Ependyma (ependymal cells) lining brain cavities and central canal in spinal cord.

Oligodendrocytes. As their name implies, oligodendrocytes have few processes. They are often found in rows between axons. The myelin sheath around axons is formed by concentric layers of oligodendrocytes plasma membrane. Each oligodendrocyte gives off several tongue- like processes that find their way to the axon, where each process wraps itself around a portion of the axon, forming an internodal segment of myelin. Each process appears to spiral around its segment of the axon in a centripetal manner, with the continued insinuation of the leading edge between the inner surface of newly formed myelin and the axon. One oligodendrocyte may myelinate one axon or several. The nucleus-containing region may be at some distance from the axon(s) it is myelinating. In the CNS, nodes of Ranvier (between

myelinated regions) are larger than those of the PNS, and the larger amount of exposed axolemma makes saltatory conduction more efficient.

Unmyelinated axons in the CNS are truly bare, that is they are not embedded in any glial cell process. (In contrast to the situation in the PNS, described below.)

Astrocytes. Astrocytes are the largest of the neuroglial cells. They have elaborate processes that extend between neurons and blood vessels. The ends of the processes expand to form end feet, which cover large areas of the outer surface of the blood vessel or axolemma. Astrocytes are believed to play a role in the movement of metabolites and wastes to and from neurons, and in regulating ionic concentrations within the neurons. They may be involved in regulating the tight junctions in the capillaries that form the blood-brain barrier. Astrocytes also cover the bare areas of neurons, at nodes of Ranvier and synapses. They may act to confine neurotransmitters to the synaptic cleft and to remove excess neurotransmitters.

Two kinds of astrocytes are identified, protoplasmic and fibrous astrocytes. Both types contain prominent bundles of intermediate filaments, but the filaments are more numerous in fibrous astrocytes. Fibrous astrocytes are more prevalent in white matter, protoplasmic ones in grey matter.

Microglia. These are the smallest of the glial cells, with short twisted processes. They are the phagocytes of the CNS, considered part of the mononuclear phagocytic system (see pg 110 in Ross et al.). They are believed to originate in bone marrow and enter the CNS from the blood. In the adult CNS, they are present only in small numbers, but proliferate and become actively phagocytic in disease and injury. Their alternate name, mesoglia, reflects their embyonic origin from mesoderm (the rest of the nervous system, including the other glial cells, is of neuroectodermal or neural crest origin).

Ependymal cells. Cuboidal to columnar cells in one layer lining the fluid-filled brain ventricles and central canal (canalis centralis) in spinal cord. Ependyma is involved in cerebrospinal fluid production in som regions (choroid plexus).

Support cells in the PNS :

The support cells of the PNS are called satellite cells and Schwann cells.

Satellite cells. Satellite cells surround the cell bodies of the neurons in ganglia (ganglion cells). These small cuboidal cells form a complete layer around the nerve cell body, but only their nuclei are visible in routine preparations. They help maintain a controlled microenvironment around the nerve cell body, providing electrical insulation and a pathway for metabolic exchange. In paravertebral and peripheral ganglia, nerve cell processes must penetrate between satellite cells to establish a synapse.

Satelite cells – nutrition and isolation of neurons in ganglia