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Enumeration of Bacteria in a Culture Lab, Lab Reports of Microbiology

This PDF contains the answers to the lab which I got a 100% on.

Typology: Lab Reports

2022/2023

Available from 08/10/2023

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Name __________________________________________ Section _____________
Bacterial Enumeration - Results & Interpretation 15 pts total
Use the results provided in the file "Enumeration of Bacteria in a Culture using the Techniques of Serial Dilution
and the Spread Plate Method - RESULTS" to complete this assignment.
7 pts for table. ** If the plate is TFTC or TMTC/TNTC you must record that in the table, don’t just
put a dash or leave blank.
Quantitative Techniques Questions 8 pts
1. You plated 0.1 ml of a 10-3 dilution an E. coli culture. After incubating for 48 hours at 37°C there
are 63 colonies on the plate. How many CFU were in the original culture? (1 pt)
PLATE Tube
dilution Volume
Plated
Final dilution
on the plate
(0.5 pt each space)
Number of
colonies
(0.5 pt each space)
CFU/ml
(0.5 pt each space)
1 10-4 0.1 ml
2 10-5 0.1 ml
3 10-6 0.1 ml
4 10-7 0.1 ml
Average -
only average the
countable plates
(1 pt)
SU23
10^-5 dilution
TMTC
N/A
10^-6 dilution
TMTC
N/A
10^-7 dilution
139 colonies
1.39 * 10^9 CFU/mL
10^-8 dilution
TFTC (28
colonies)
N/A
Alexa Campanella
63 / (10^-3 * 0.1) =
630,000 CFU
pf3

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Name __________________________________________ Section _____________

Bacterial Enumeration - Results & Interpretation – 15 pts total Use the results provided in the file "Enumeration of Bacteria in a Culture using the Techniques of Serial Dilution and the Spread Plate Method - RESULTS" to complete this assignment.

7 pts for table. **** If the plate is TFTC or TMTC/TNTC you must record that in the table, don’t just put a dash or leave blank**.

Quantitative Techniques – Questions – 8 pts

  1. You plated 0.1 ml of a 10-3^ dilution an E. coli culture. After incubating for 48 hours at 37°C there are 63 colonies on the plate. How many CFU were in the original culture? (1 pt)

PLATE Tube dilution

Volume Plated

Final dilution on the plate (0.5 pt each space)

Number of colonies (0.5 pt each space)

CFU/ml (0.5 pt each space)

1 10 -4^ 0.1 ml

2 10 -5^ 0.1 ml

3 10 -6^ 0.1 ml

4 10 -7^ 0.1 ml

Average - only average the countable plates

(1 pt)

  1. You plated 0.1 ml of a 10-^5 dilution an E. coli culture. After incubating for 48 hours at 37°C there

are 27 colonies on the plate. How many CFU were in the original culture?

  1. You plated 0.1 ml of a 10-^7 dilution an E. coli culture. After incubating for 48 hours at 37°C there

are 345 colonies on the plate. How many CFU were in the original culture?

  1. You plated 100 μl of a 10-^4 dilution an E. coli culture. After incubating for 48 hours at 37°C there

are 88 colonies on the plate. How many CFU were in the original culture?

(1 pt)

(1 pt)

(1 pt)