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DNA sequencing.pdf, Lecture notes of Genetics

University of Antelope Valley Genetics

The new RNA molecule is formed by incorporating. • nucleotides that are complementary to the template strand. DNA coding strand. DNA template strand. DNA.

Typology: Lecture notes

2021/2022

Uploaded on 09/12/2022

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Gel electrophoresis

DNA sequencing

Cytoplasm

Nucleus

DNA

DNA is the genetic material

within the nucleus.

Central Dogma

RNA

Protein

Replication

The process of replication

creates new copies of DNA.

Transcription

The process of transcription

creates an RNA using

DNA information.

Translation

The process of translation

creates a protein using

RNA information.

DNA Double Helix-Held Together with

H-Bonds

Partial preview of the text

Download DNA sequencing.pdf and more Lecture notes Genetics in PDF only on Docsity!

Gel electrophoresis

DNA sequencing

Cytoplasm

Nucleus

DNA

DNA is the genetic materialwithin the nucleus.

Central Dogma

RNA Protein

Replication

The process of replicationcreates new copies of DNA.

Transcription

The process of transcriptioncreates an RNA usingDNA information.

Translation

The process of translationcreates a protein usingRNA information.

DNA Double Helix-Held Together with

H-Bonds

Base Pairs Double Helix

base: thymine(pyrimidine)

sugar: 2’-deoxyribose

monophosphate

no 2’-hydroxyl

(5’ to 3’)

5’ 3’

base:adenine(purine)

1’2’

4’

α 3’ linkage

5’ linkage

Three Components of DNA Structure

Pyrimidines used in Base Pairs,

DNA

6-membered rings only

Purines used in Base Pairs, DNA

Fused 5 and 6 member rings

A-T and G-C Base Pairs Hold the

DNA helices together

A-T and G-C Base Pairs Hold the

DNA helices together

Hydrogen-Bonding’s Role in DNA Structure

Transcription

•^

The new RNA molecule is formed by incorporating

•^

nucleotides that are complementary to thetemplate strand.

DNA coding strand

DNA template strand

DNA

G T

C A

T T

C G G

C A G T A A G C C

G

RNA

G

G U C A U U C

# of strandskind of sugar bases used

RNA Polymerase is the Enzyme that CatalyzesTranscription of DNA Information to RNA

DNA (Blue)Newly SynthesizedRNA (Red)Bridge Helix MovesDNA throughPolymerase duringRNA Synthesis(Green)Active Site Metal(Pink)

Transcription

•^

The new RNA molecule is formed by incorporating

•^

nucleotides that are complementary to thetemplate strand.

DNA coding strand

DNA template strand

DNA

G T

C A

T T

C G G

C A G T A A G C C

G

RNA

G

G U C A U U C

Translation

The process of reading the RNA sequence of anmRNA and creating the amino acid sequence of aprotein is called translation.

Transcription

Codon

Translation

DNA

T^

T

C

A

G

T

C

A

G

DNAtemplatestrand

mRNA

A

G

U

C

A

G

U

C

MessengerRNA

Protein

Lysine

Serine

Valine

Polypeptide(amino acidsequence)

http://www.dnalc.org/ddnalc/resources/sangerseq.html

http://www.shsu.edu/~chm_tgc/sounds/flashfiles/GE.swf

Shotgun sequencing: assembly of random sequence fragments

DNA sequencing.pdf, Lecture notes of Genetics

Related documents

Partial preview of the text

Download DNA sequencing.pdf and more Lecture notes Genetics in PDF only on Docsity!

Gel electrophoresis

DNA sequencing

DNA is the genetic materialwithin the nucleus.

Central Dogma

The process of replicationcreates new copies of DNA.

The process of transcriptioncreates an RNA usingDNA information.

The process of translationcreates a protein usingRNA information.

DNA Double Helix-Held Together with

H-Bonds

Base Pairs Double Helix

Three Components of DNA Structure

Pyrimidines used in Base Pairs,

DNA

6-membered rings only

Purines used in Base Pairs, DNA

Fused 5 and 6 member rings

A-T and G-C Base Pairs Hold the

DNA helices together

A-T and G-C Base Pairs Hold the

DNA helices together

Hydrogen-Bonding’s Role in DNA Structure

Transcription

•^

The new RNA molecule is formed by incorporating

•^

nucleotides that are complementary to thetemplate strand.

DNA

G T

C A

T T

C G G

C A G T A A G C C

G

RNA

G

G U C A U U C

# of strandskind of sugar bases used

RNA Polymerase is the Enzyme that CatalyzesTranscription of DNA Information to RNA

DNA (Blue)Newly SynthesizedRNA (Red)Bridge Helix MovesDNA throughPolymerase duringRNA Synthesis(Green)Active Site Metal(Pink)

Transcription

•^

The new RNA molecule is formed by incorporating

•^

nucleotides that are complementary to thetemplate strand.

DNA

G T

C A

T T

C G G

C A G T A A G C C

G

RNA

G

G U C A U U C

Translation

The process of reading the RNA sequence of anmRNA and creating the amino acid sequence of aprotein is called translation.

DNA

T^

T

C

A

G

T

C

A

G

A

A

G

U

C

A

G

U

C