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DNA Replication: A Guided Exercise for Biology Students, Exercises of Genetics

Below are the steps of DNA replication which take place during the S stage of Interphase. In each box, read what is taking place and follow the.

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DNA Replication Illustrations Name Per. ________ Date ________
Below are the steps of DNA replication which take place during the S stage of Interphase. In each box, read what is taking place and follow the
given directions. Pay attention to the specific coloring directions. Use chapter 12 (Section 2) in your book to help you.
DOUBLE HELIX MOLECULE
Label the following:
Nitrogen Base Pair
Sugar/Phosphate Backbone
Color: Draw a thick BLUE line
over each helix strand to
represent the original strands
UNWOUND DNA MOLECULE
Label the following:
Deoxyribose Sugar
Phosphate
The “Missing” Bases
Color:
Sugars ORANGE
A’s GREEN ; T’s PINK
C’s – RED ; G’s - PURPLE
UNZIPPED DNA MOLECULE
Label the following:
The “Missing” Bases
DNA Helicase
Color:
Sugars ORANGE
A’s GREEN ; T’s PINK
C’s – RED ; G’s - PURPLE
REPLICATING DNA
Label the following:
The “Newly Added” Bases
DNA Polymerase (2x)
Color:
All original bases BLUE
All newly added bases YELLOW
REPLICATED DNA
Color:
(Keep in mind, DNA spirals)
Original Backbone BLUE
New Backbone YELLOW
G
A
T
C
C
G
T
T
A
C
Old
New
New
C
T
A
G
G
C
A
A
T
G
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DNA Replication Illustrations Name Per. ________ Date ________

Below are the steps of DNA replication which take place during the S stage of Interphase. In each box, read what is taking place and follow the

given directions. Pay attention to the specific coloring directions. Use chapter 12 (Section 2) in your book to help you.

DOUBLE HELIX MOLECULE

Label the following: Nitrogen Base Pair Sugar/Phosphate Backbone Color: Draw a thick BLUE line over each helix strand to represent the original strands

UNWOUND DNA MOLECULE

Label the following: Deoxyribose Sugar Phosphate The “Missing” Bases Color: Sugars – ORANGE A’s – GREEN ; T’s – PINK C’s – RED ; G’s - PURPLE

UNZIPPED DNA MOLECULE

Label the following: The “Missing” Bases DNA Helicase Color: Sugars – ORANGE A’s – GREEN ; T’s – PINK C’s – RED ; G’s - PURPLE

REPLICATING DNA

Label the following: The “Newly Added” Bases DNA Polymerase (2x) Color: All original bases BLUE All newly added bases YELLOW

REPLICATED DNA

Color: (Keep in mind, DNA spirals) Original Backbone BLUE New Backbone YELLOW G A T C C G T T A C

Old New

New Old

C T A G G C A A T G

Name ___________________________________________________ Date ___________________ Period ______

DNA Replication Notes

  1. Observe the model of DNA. Use the box below to write what you know about its structure.
  2. Can you remember? When does DNA replication take place during the cell cycle (be specific!)? S STAGE OF INTERPHASE
  3. Where does replication DNA replication take place within the cell? Hint: think about where DNA is found! NUCLEUS
  4. Form a hypothesis. How do you think a cell goes from having one molecule of DNA to two molecules of DNA? Keep in mind, it is very important for these molecules to be completely identical to each other. DNA Replication – The Process
  5. What is the overall purpose of DNA replication?

DNA  DNA

  1. In order for replication to take place, DNA must first unwind and ____ UNZIP ___. a. Which enzyme is responsible for this? DNA HELICASE b. The sites where DNA “separates” for replication are known as ____ REPLICATION FORKS _____.
  2. Each original strand of DNA will now serve as a ___ TEMPLATE _______ for the new strand.
  3. The new, ___ COMPLEMENTARY ____ nucleotides must now be added to the original strand. a. Which enzyme is responsible for building the new strand? DNA POLYMERASE
  4. If the original strand of DNA contains the following sequence: G T C A T T C G A , what would be the sequence of complementary nucleotides added during replication? CAG TAA GCT
  5. In reality, this is taking place in many locations at once. This creates __ OKAZAKI FRAGMENTS ____. a. Which enzyme is responsible for putting these pieces together?

DNA LIGASE

SHAPE – DOUBLE HELIX

BACKBONE – ALTERNATING SUGARS AND PHOSPHATES

STEPS – NITROGEN BASE PAIRS ATTACHED TO SUGAR

ADENINE PAIRS WITH THYMINE

CYTOSINE PAIRS WITH GUANINE