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DNA Manipulation | BIOL - Cell Biology 1 - Introduction, Quizzes of Cell Biology

Class: BIOL - Cell Biology 1 - Introduction; Subject: Biology / Biological Sciences; University: Medaille College; Term: Forever 1989;

Typology: Quizzes

2014/2015

Uploaded on 04/27/2015

pshcaitlinx3
pshcaitlinx3 🇺🇸

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TERM 1
What is recombinant DNA technology?
DEFINITION 1
What is genetic engineering?
TERM 2
Know the steps in generating recombinant
DNA?
DEFINITION 2
What are vetors and where are vectors most often from?
TERM 3
What are clones?
DEFINITION 3
What is the alternate name for restriction enzymes? Where
are they isolated from?
TERM 4
What is the function of restriction enzymes in
bacteria?
DEFINITION 4
How do bacteria protect their own DNA?
TERM 5
What do restriction enzymes
recognize?
DEFINITION 5
Differentiate between Type 1 and Type 2 restriction
endonucleases. Which recognizes a palindromic DNA
sequence? Which is most likely to be used in genetic
engineering? Why?
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What is recombinant DNA technology?

What is genetic engineering?

TERM 2

Know the steps in generating recombinant

DNA?

DEFINITION 2

What are vetors and where are vectors most often from?

TERM 3

What are clones?

DEFINITION 3

What is the alternate name for restriction enzymes? Where

are they isolated from?

TERM 4

What is the function of restriction enzymes in

bacteria?

DEFINITION 4

How do bacteria protect their own DNA?

TERM 5

What do restriction enzymes

recognize?

DEFINITION 5

Differentiate between Type 1 and Type 2 restriction

endonucleases. Which recognizes a palindromic DNA

sequence? Which is most likely to be used in genetic

engineering? Why?

What is the palindromic DNA sequence that

EcoR1 cleaves?

Do restriction enzymes all recognize the same DNA

sequence?

TERM 7

What is the difference between blunt and

sticky ends?

DEFINITION 7

Example of the creation of a blunt end

TERM 8

Example of the creation of a sticky end

DEFINITION 8

What is meant by sticky ends? Can they be rejoined? How?

TERM 9

Can blunt ends be

rejoined?

DEFINITION 9

Describe the process of gel electrophoresis? Do smaller or

larger fragments move faster?

TERM 10

What are the steps of gel

electrophoresis?

DEFINITION 10

What is molecular cloning? What are the steps? What are

DNA clones?

What are the steps of library construction? Do

they contain SINEs and LINEs?

What is cDNA? How is a cDNA library made? What enzyme is

used to accomplish this task? Where was the enzyme

discovered?

TERM 17

Describe the steps of PCR. For what is it used?

DEFINITION 17

To what bacterial clones transferred? With what is the filter

paper treated? How do you identify colonies that bind to the

probe?

TERM 18

Who won a nobel prize for developing PCR

and in what year?

DEFINITION 18

What enzyme is added to PCR?

TERM 19

What is a thermocycler? What temperature is

optimal for the enzyme?

DEFINITION 19

At the end of the cycle byhow muchhave you increased the

quantity of DNA?