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Gene Expression Regulation and Analysis: A Focus on MicroRNAs and Arabidopsis, Slides of Botany and Agronomy

The regulation of gene expression in plants, with a focus on micrornas and their role in gene silencing. It also covers various technologies used in gene identification, including rnai, mutational studies, transposon tagging, and microarray analysis. Information about arabidopsis, transposable elements, and the use of reporter genes gus and gfp in gene expression studies.

Typology: Slides

2012/2013

Uploaded on 01/09/2013

prakash
prakash 🇮🇳

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Differential Gene Expression & plant
development
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Differential Gene Expression & plant development

Regulation of gene expression

  • Environmental stimuli
  • Hormones
  • Transcription factors
  • microRNAs

MicroRNAs

  • Overexpression of miR

microRNAs and RNAi

  • RNAi – RNA interference
    • Used to determine the consequences of down- regulating gene expression

Arabidopsis

Transposable elements

Transposon

tagging

Transposon tagging utilizes colorimetric expression assays

  • GUS reporter gene (B-glucuronidase), E. coli
  • GFP (green fluorescent protein), jellyfish

Microarrays and development

  • Example
    • Vegetative meristem mRNA labeled green
    • Flower bud mRNA labeled red