Different types of PCR Questions with
Accurate Answers
Hot-Start PCR
advantages correct answer Reduces primer-dimer formation
Reduces non-specific bidning to other sequences in target mispriming.
Hot-Start PCR
Procedures correct answer Pre-warm thermocycler before adding reagents
or
Withold the DNA polymerase from the rxn until the temp rises above the Tm of the reactants by using a
wax bead
or
Use a commerical DNA polymerase supplied in inactive form until activated by heat in the denaturation
step
Hot-Start PCR
Principle correct answer The rxn does not begin until the denaturation temperature is reached.
Multiplex PCR
Application (forensic) correct answer ID multiple markers
Multiplex PCR
Applications (Clinical) correct answer Can ID multiple pathogens in a single sample
