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COVID-19 QUESTIONS WITH ACCURATE ANSWERS, Exams of Biology

COVID-19 QUESTIONS WITH ACCURATE ANSWERS

Typology: Exams

2024/2025

Available from 06/11/2025

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COVID-19 QUESTIONS WITH ACCURATE
ANSWERS
-To which host cell surface receptor does the Spike protein bind?
-Where are these cell receptors located in humans?
-How does this explain the diversity of signs and symptoms people experience when suffering from
Covid-19 infections? correct answer -Protein called ACE2 (enzyme that reduces BP)
-found in lungs, heart, blood vessels
-Depending on where and which tissues the spikes attach to, you could have a variety of symptoms like
respiratory failure, heart failure, arrythmia, vascular inflammation, and more
-Which special enzyme would copy the Covid-19 RNA within a human cell?
-Do human cells have genes for this enzyme?
-Must the Covid-19 virus carry the genetic info for this enzyme?
-Does this enzyme proofread/edit? Consequences? correct answer RNA dependent RNA polymerase; no;
yes; Many mutations
Are coronaviruses RNA or DNA viruses? correct answer RNA viruses
Are they naked or enveloped? correct answer enveloped
Can our vaccines still protect us from this variant, so far? correct answer YES
Describe the probe/reporter used in the UK Thermo TaqPath COVID-19 RT- PCR? How is fluorescence
used in this test? correct answer -Developed 3 probes that bind to different spots of the viral genome
with three different colors.
-Once all three surpass the threshold, the test is positive.
-Lower the Ct value means more virus.
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COVID-19 QUESTIONS WITH ACCURATE

ANSWERS

-To which host cell surface receptor does the Spike protein bind? -Where are these cell receptors located in humans? -How does this explain the diversity of signs and symptoms people experience when suffering from Covid-19 infections? correct answer -Protein called ACE2 (enzyme that reduces BP) -found in lungs, heart, blood vessels -Depending on where and which tissues the spikes attach to, you could have a variety of symptoms like respiratory failure, heart failure, arrythmia, vascular inflammation, and more -Which special enzyme would copy the Covid-19 RNA within a human cell? -Do human cells have genes for this enzyme? -Must the Covid-19 virus carry the genetic info for this enzyme? -Does this enzyme proofread/edit? Consequences? correct answer RNA dependent RNA polymerase; no; yes; Many mutations Are coronaviruses RNA or DNA viruses? correct answer RNA viruses Are they naked or enveloped? correct answer enveloped Can our vaccines still protect us from this variant, so far? correct answer YES Describe the probe/reporter used in the UK Thermo TaqPath COVID-19 RT- PCR? How is fluorescence used in this test? correct answer -Developed 3 probes that bind to different spots of the viral genome with three different colors. -Once all three surpass the threshold, the test is positive. -Lower the Ct value means more virus.

How did these evolve? Significance? How does the S test dropouts help monitor spread of the B1.1. variant? correct answer Can monitor how many people are testing for that specific variant in different areas which allows to see where the variant has spread to and how strict to make lockdown measures If a person made antibodies which could bind to the Spike protein, what would happen or not happen? correct answer They would recognize the virus and initiate an immune response when it binds to receptors of the cells In describing the Covid-19 coronavirus, of what advantages is an RNA genome compared to a DNA genome? correct answer Easier to package into a virus than DNA, lighter, RNA far more prone to mutation Is the COvid-19 genome larger or smaller than the human genome? What is the consequence of this small genome? correct answer Smaller; Does not have genes to replicate on its own, without a host; contains absolute minimum to make up a virus Lateral flow test: correct answer -pieces of paper with different chemicals on them and they're trying to detect viral rna or antibodies. -Rapid test. Hour or less Outside of the host, would the Covid-19 virus remain infectious for long? correct answer Not long because its lipid membrane dries out quickly RT-PCR test: correct answer -Looks for viral RNA in a patients airways; -sample collected with cotton tip, sits in fridge, RNA extracted from the sample, RNA in tube and make a DNA copy of the virus genome, -Real-time PCR; 1-2 days What are probes/reporters? correct answer -Short DNA primer that is added which binds to a part of the viral RNA and has a fluorescent chemical on it. -Polymerase cleaves the chemical so that it is not connected to the quencher.

What is the goal of PCR? correct answer Looks for viral RNA our DNA genome and makes lots of copies of the known DNA sequence What is the S. African variant B1.135? correct answer new variants with the N501Y mutation which spread faster and may increase ACE2 binding What is the SARS-CoV-2 virus? correct answer A coronavirus What is the UK variant B. 1.1.7? correct answer have altered spike recognition by antibodies, impacting some antibody therapies What must one mix together to run a PCR? correct answer DNA template, primers, 4 nucleotide bases, DNA polymerase enzyme Which clinical samples could be used for these tests? correct answer snot swab Which enzyme is used to convert the Covid-19 virus RNA into a DNA copy? Why is this necessary for PCR? correct answer Bacterial DNA polymerase Because if viral RNA sticks and the polymerase is put to work , then we know the virus is present Which mutations could increase transmission of the Covid-19 virus? correct answer Mutation in spike proteins Which mutations may decrease the ability of neutralizing antibodies to bind to the Covid-19 virus? correct answer Mutation in spike proteins that change the surface of the spike Which of the tests would have the lowest sensitivity, greatest chances of "false negative results"? correct answer Lateral flow test Which special type of DNA polymerase is used? Why? correct answer One that works at high temperatures (from bacteria that live in hot waters) because you later heat the PCR mix

Which tests would be used to detect current infections? past infections? correct answer PCR test for viral RNA; PCR test for viral antibodies Which variant is spreading rapidly throughout the US? correct answer UK variant B. 1.1.7 and S. African variant B1. Why would some mutations be harmless and others more dangerous for humans? correct answer - Some are silent mutations that do not affect the COVID-19 disease and help scientists track the evolution and spread of the virus around the world. -Dangerous because some may improve viral transmission or performance.