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Material Type: Notes; Class: Basic Biochemistry; Subject: Chemistry; University: Lourdes College; Term: Summer I 1999;
Typology: Study notes
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Course Outline CHM 305 Basic Biochemistry May 1999
Department: Chemistry and Physical Sciences Credit Hours: 3 Prerequisite: CHM/CHL 302 or approval of instructor General Education: 7.1 Scientific Literacy College Learning Outcomes: Scientific Literacy 7a-d, Critical Thinking 2a,b
Course Description Includes the chemistry of living systems, considers carbohydrates, lipids, nucleic acids, and proteins.
Purpose of the Course To introduce the student to the basic concepts and language of biochemistry.
College Learning Outcomes and Objectives: L.O. 7 Scientific Literacy โ Graduates can demonstrate an understanding of natural and behavioral scientific principles, technology, and methods. a. They can distinguish between the qualitative and quantitative characteristics of natural phenomena. b. They can apply scientific principles and methods to support or disprove hypotheses. c. They can use theories to explain past observations and to predict answers to new questions. d. They can understand the uses of scientific technology and their implications.
L.O. 2 Critical Thinking Ability โ Graduates can analyze issues and theories rationally, logically, and coherently using both qualitative and quantitative information. a. They can demonstrate an explicit understanding of principles of critical thought. b. They can demonstrate the ability to reflect on issues and/or theories systematically
Course Objectives
In order to gain mastery in the field of biochemistry, the student should be able to:
Topical Outline I. Water, pH and Ionic Equilibria A. Properties of water B. pH
II. Amino acids
A. Structure of amino acids B. Acid-base chemistry C. Reactions D. Optical activity and stereochemistry
III. Proteins: Biological Functions and Structure A. Formation of the peptide bond B. Characteristics of the peptide bond C. Biological functions of proteins D. Primary structure E. Forces influencing protein structure
IV. Lipids and Membranes A. Classes of lipids
V. Carbohydrates A. Nomenclature B. Monosaccharides
F. Physical significance of thermodynamic properties G. Effect of pH on standard-state free energies H. Effect of concentration on net free energy changes I. Coupled processes in living things J. Energy-rich compounds
VIII. Overview of metabolism A. Carbon and nitrogen cycles B. Catabolism C. Anabolism D. Role of NAD+, NADP+, and FAD
IX. Glycolysis A. Importance of coupled reactions B. First phase (reactions 1-5) C. Second phase (reactions 6-10) D. Metabolic fates of NADH and pyruvate E. Anaerobic pathways for pyruvate F. Regulation of glycolysis G. Utilization of other substrates in glycolysis
X. The tricarboxylic acid (TCA) cycle A. Overview of the TCA cycle B. Bridging step between glycolysis and the TCA cycle C. Reactions of the TCA cycle D. Regulation of the TCA cycle E. Anaplerotic reactions
XI. Electron transport and oxidative phosphorylation A. Reduction potentials B. Overview of the electron transport chain C. Reactions of the electron transport chain D. Chemiosmotic coupling โ formation of ATP E. Inhibitors of oxidative phosphorylation F. Uncouplers that disrupt the coupling of the electron transport and ATP synthase G. Shuttle systems for cytosolic NADH H. ATP-ADP translocase
XII. Gluconeogenesis, glycogen metabolism and the pentose phosphate pathway A. Gluconeogenesis reactions B. Regulation of gluconeogenesis C. Glycogen catabolism D. Glycogen synthesis E. Control of glycogen metabolism F. Pentose phosphate pathway
XIII. Fatty acid catabolism A. Mobilization of fats from dietary intake and adipose tissue B. Reactions of beta oxidation of fatty acids C. Beta oxidation of odd-carbon fatty acids D. Beta oxidation of unsaturated fatty acids E. Ketone bodies
XIV. Nucleotides and nucleic acids
A. Components of nucleotides
XV. DNA replication General features of DNA replication